Use of real-time PCR to measure Epstein–Barr virus genomes in whole blood

2002 ◽  
Vol 270 (2) ◽  
pp. 259-267 ◽  
Author(s):  
E Leung ◽  
B.K Shenton ◽  
G Jackson ◽  
F.K Gould ◽  
C Yap ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Whole Blood ◽  
Epstein Barr Virus ◽  
Barr Virus ◽  
Epstein Barr ◽  
Virus Genomes

scholarly journals Comparison of Commercial Real-Time PCR Assays for Quantification of Epstein-Barr Virus DNA

2005 ◽  
Vol 43 (5) ◽  
pp. 2053-2057 ◽  
Author(s):  
G. Ruiz ◽  
P. Pena ◽  
F. de Ory ◽  
J. E. Echevarria
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Epstein Barr Virus ◽  
Barr Virus ◽  
Pcr Assays ◽  
Epstein Barr

Quantitation of Epstein-Barr virus mRNA using reverse transcription and real-time PCR

2004 ◽  
Vol 74 (4) ◽  
pp. 612-618 ◽  
Author(s):  
Birgit Weinberger ◽  
Annelie Plentz ◽  
Klaus M. Weinberger ◽  
Joachim Hahn ◽  
Ernst Holler ◽  
...  
Keyword(s):  
Real Time ◽  
Reverse Transcription ◽  
Real Time Pcr ◽  
Epstein Barr Virus ◽  
Barr Virus ◽  
Epstein Barr

scholarly journals Heterogeneous, restricted patterns of Epstein–Barr virus (EBV) latent gene expression in patients with chronic active EBV infection

2001 ◽  
Vol 82 (10) ◽  
pp. 2385-2392 ◽  
Author(s):  
Mikio Yoshioka ◽  
Nobuhisa Ishiguro ◽  
Hiroaki Ishiko ◽  
Xiaoming Ma ◽  
Hideaki Kikuta ◽  
...  
Keyword(s):  
Gene Expression ◽  
T Cell ◽  
Real Time ◽  
Real Time Pcr ◽  
Epstein Barr Virus ◽  
Clinical Symptoms ◽  
The Other ◽  
Early Gene ◽  
Barr Virus ◽  
Epstein Barr

Epstein–Barr virus (EBV) has been shown to infect T lymphocytes and to be associated with a chronic active infection (CAEBV), which has been recognized as a mainly non-neoplastic T-cell lymphoproliferative disorder (T-cell LPD). The systemic distribution of EBV genomes was studied, by real-time PCR, in multiple tissues from six patients with CAEBV, including three patients with T-cell LPD, one patient with B-cell LPD and two patients with undetermined cell-type LPD. There were extremely high loads of EBV genomes in all tissues from the patients. This reflects an abundance of circulating and infiltrating EBV-infected cells and a wide variety of clinical symptoms in the affected tissues. We chose one sample from each patient that was shown by real-time PCR to contain a high load of EBV genomes and examined the expression of EBV latent genes by RT–PCR. EBER1 and EBNA1 transcripts were detected in all samples. Only one sample also expressed EBNA2, LMP1 and LMP2A transcripts in addition to EBER1 and EBNA1 transcripts. Two of the remaining five samples expressed LMP1 and LMP2A transcripts. One sample expressed LMP2A but not LMP1 and EBNA2 transcripts. Another sample expressed EBNA2 but not LMP1 and LMP2A transcripts. The other sample did not express transcripts of any of the other EBNAs or LMPs. None of the samples expressed the viral immediate-early gene BZLF1. These results showed that EBV latent gene expression in CAEBV is heterogeneous and that restricted forms of EBV latency might play a pathogenic role in the development of CAEBV.

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