Acaricidal activity of natural monoterpenes on Tyrophagus putrescentiae (Schrank), a mite of stored food

AbstractAcaricidal activities and color alterations of 5-methylfurfural derived from Valeriana fauriei essential oil and its structural analogues against Dermatophagoides farinae, D. pteronyssinus, Haemaphysalis longicornis and Tyrophagus putrescentiae were evaluated in the present study. Based on the LD50 values of 5-methylfurfural and its analogues, 4,5-dimethylfurfural showed the highest acaricidal activity (LD50; 9.95, 9.91, and 7.12 μg/cm2), followed by 5-methylfurfural (11.87, 11.00, and 8.59 μg/cm2), furfural (12.94, 13.25, and 10.36 μg/cm2), and V. fauriei essential oil (15.15, 13.64, and 10.14 μg/cm2) against D. farinae, D. pteronyssinus and T. putrescentiae, respectively. However, all tested compounds did not show the acaricidal activities against H. longicornis. Interestingly, the color alterations of the mites and ticks were observed by furfural, 5-methylfurfural, and 4,5-dimethylfurfural from colorless to red brown during the acaricidal experiments. Furthermore, 4,5-dimethylfurfural which exhibited the highest acaricidal activity was formulated as nanoemulsion. The nanoemulsion of 4,5-dimethylfurfural showed higher acaricidal activity than it was emulsified in ethanol. The nanoemulsion was also found to show color changes of the mites and ticks from colorless to red brown. The results suggest that 5-methylfurfural and its analogues could be developed as an effective and easy-to-recognize acaricides to mites and ticks.

Download Full-text

Food Protective Effects of the Active Constituent Isolated from Ostericum praeteritum against the Stored Food Mite, Tyrophagus putrescentiae

Journal of Food Protection ◽

10.4315/0362-028x.jfp-13-212 ◽

2013 ◽

Vol 76 (11) ◽

pp. 1887-1891

Author(s):

MIN-GI KIM ◽

JU-HYUN JEON ◽

HOI-SEON LEE

Keyword(s):

Lethal Dose ◽

Acaricidal Activity ◽

Contact Toxicity ◽

Protective Effects ◽

Tyrophagus Putrescentiae ◽

Toxicity Bioassay ◽

Benzyl Benzoate ◽

Active Constituent ◽

Toxic Compound ◽

Chromatographic Techniques

The acaricidal effects of the active constituent isolated from Ostericum praeteritum oil and its derivatives were compared with that of benzyl benzoate using fumigant and contact toxicity bioassays against Tyrophagus putrescentiae mites. The active compound of O. praeteritum was isolated by various chromatographic techniques and was identified as 3-methylphenol (C7H8O). On the basis of 50% lethal dose values, 3-methylphenol (1.42 μg/cm2) was determined to be 9.18 times more effective than benzyl benzoate (13.04 μg/cm2), followed by 6-fluoro-3-methylphenol (2.18 μg/cm2), 4-isopropyl-3-methylphenol (2.53 μg/cm2), and 6-chloro-3-methylphenol (4.03 μg/cm2), against Tyrophagus putrescentiae mites in the fumigant bioassay. In the contact toxicity bioassay, 3-methylphenol (1.03 μg/cm2) was the most-toxic compound against T. putrescentiae mites, followed by 6-fluoro-3-methylphenol (2.09 μg/cm2), 4-isopropyl-3-methylphenol (2.11 μg/cm2), 6-chloro-3-methylphenol (3.78 μg/cm2), and benzyl benzoate (10.33 μg/cm2). These results indicate that the introduction of chloro, isopropyl, and fluoro functional groups to the 3-methylphenol skeleton increased the acaricidal activity. Therefore, 3-methylphenol and its derivatives could potentially be used as natural acaricides against T. purescentiae.

Download Full-text

Acaricidal Activities of Materials Derived from Pyrus ussuriensis Fruits against Stored Food Mites

Journal of Food Protection ◽

10.4315/0362-028x.jfp-12-040 ◽

2012 ◽

Vol 75 (7) ◽

pp. 1258-1262 ◽

Cited By ~ 6

Author(s):

JU-HYUN JEON ◽

JI-YEON YANG ◽

HOI-SEON LEE

Keyword(s):

Ethyl Acetate ◽

Aqueous Extract ◽

Filter Paper ◽

Lethal Dose ◽

Ethyl Acetate Fraction ◽

Acaricidal Activity ◽

Tyrophagus Putrescentiae ◽

Benzyl Benzoate ◽

Chromatographic Techniques

The acaricidal activities of materials derived from Pyrus ussuriensis fruits were evaluated against Tyrophagus putrescentiae and compared with that of commercial acaricide (benzyl benzoate). On the basis of the 50% lethal dose (LD50) values, the ethyl acetate fraction of the fractions obtained from an aqueous extract of P. ussuriensis fruits had the highest acaricidal activity (16.32 μg/cm2) against T. putrescentiae. The acaricidal constituent of P. ussuriensis fruits was isolated by chromatographic techniques and identified as 1,4-benzoquinone. On the basis of the LD50 values, 1,4-benzoquinone (1.98 μg/cm2) was 5.9 times more toxic than benzyl benzoate (11.69 μg/cm2), followed by 2-isopropyl-5-methyl-1,4-benzoquinone (3.29 μg/cm2), and 2,3-dimethoxy-5-methyl-1,4-benzoquinone (5.03 μg/cm2) against T. putrescentiae in the fumigant bioassay. In a filter paper bioassay, the acaricidal activity of 1,4-benzoquinone (0.07 μg/cm2) was 120.1 times more effective than that of benzyl benzoate (8.41 μg/cm2), followed by 2-isopropyl-5-methyl-1,4-benzoquinone (0.11 μg/cm2) and 2,3-dimethoxy-5-methyl-1,4-benzoquinone (0.30 μg/cm2) against T. putrescentiae. These results demonstrate that P. ussuriensis fruit–derived material and its derivatives have potential as new preventive agents for the control of stored food mites.

Download Full-text