The inflammatory macrophage—Concanavalin A interaction: A thin-section and scanning electron microscopy and laser doppler electrophoretic investigation of surface events

Based on the comprehensive utilization of core, thin section identification, scanning electron microscopy and other technical means, through the YS2, 3 well fields of volcanic rock lithology and lithofacies analysis; understand that the formation and evolution of volcanic reservoirs play a constructive role and destructive effect ，which has important guiding significance[1,2].

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Surface structure of the golgi complex and identification of concanavalin-A binding sites

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100082091 ◽

1978 ◽

Vol 36 (2) ◽

pp. 246-247

Author(s):

J.M. Sturgess ◽

M. Teitelman ◽

M.A. Moscarello

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Golgi Complex ◽

Concanavalin A ◽

Binding Sites ◽

Lectin Binding ◽

Bicarbonate Buffer ◽

Sodium Phosphate Buffer ◽

Scanning Electron ◽

Lectin Binding Sites

Scanning electron microscopy has been applied to study the surface ultrastructure of the Golgi complex and labelling techniques have been developed to investigate the distribution of lectin-binding sites on the membrane surfaces. The study is based on the examination of Golgi-rich fractions, isolated by homogenisation and differential centrifugation of rat liver. The membranes are fixed in suspension with 1% glutaraldehyde in 0.1 M sodium phosphate buffer, pH 7.4 for 60 mins and then rinsed in distilled water. For scanning electron microscopy, a thin film of membrane is frozen rapidly on coverglasses using liquid Freon 22, cooled by liquid nitrogen and dried in vacuo at -60°C. Membranes are coated with approximately 100 Å gold in a sputter coater and examined at 20 kV in a JEOL JSM-35U scanning electron microscope. For transmission electron microscopy, membranes are processed as described previously. For examination of lectin binding sites, isolated Golgi membranes are washed in sodium bicarbonate buffer, fixed in glutaraldehyde, incubated with concanavalin A (Con A), rinsed in buffer and then incubated with hemocyanin1.

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Clastic Rock Reservoir Physical Characteristics of Southern Songliao Basin Changling Fault Depression Layer

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.838-841.1320 ◽

2013 ◽

Vol 838-841 ◽

pp. 1320-1323

Author(s):

Yun Feng Zhang ◽

Tian Tian Chu ◽

Hong Qi Yuan

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Thin Section ◽

Secondary Porosity ◽

Fracture Characteristics ◽

Clastic Rock ◽

Mercury Intrusion ◽

Thin Section Analysis ◽

Section Analysis ◽

Scanning Electron

We study the Changling fault depression layer reservoir space type and characteristic of clastic rock reservoirs in the study area, by core observation, thin section analysis, cathodoluminescence analysis, scanning electron microscopy and mercury intrusion method, and discusses the development of secondary porosity and fracture characteristics and formation mechanism.

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Dissolution Phenomenon of Laumontite in the Carboniferous Volcanic Oil and Gas Reservoirs of Santanghu Basin in Xinjiang

Applied Mechanics and Materials ◽

10.4028/www.scientific.net/amm.737.823 ◽

2015 ◽

Vol 737 ◽

pp. 823-826

Author(s):

He Du ◽

Wen Guang Wang ◽

Cheng Zhi Liu ◽

Jian Fei Ji

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Thin Section ◽

Exploratory Study ◽

Oil And Gas ◽

Gas Reservoirs ◽

Volcanic Reservoir ◽

Oil And Gas Reservoirs ◽

Volcanic Reservoirs ◽

Scanning Electron

Based on the data of core, thin section, scanning electron microscopy and geochemical analysis.etc, from the dissolution phenomenon of laumontite in the carboniferous volcanic reservoirs of Santanghu Basin,the exploratory study of alkaline diagenesis and the formation mechanism in volcanic reservoir was carried out.

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Fixation and conductive staining by tannin-RuO4 for transmission and scanning electron microscopy

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100157656 ◽

1990 ◽

Vol 48 (3) ◽

pp. 24-25

Author(s):

Gen Takahashi

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Thin Section ◽

Tannic Acid ◽

Osmium Tetroxide ◽

Glutaraldehyde Fixation ◽

Ruthenium Tetroxide ◽

Cellular Ultrastructure ◽

Scanning Electron

Ruthenium tetroxide(RuO4) was first used as a stain in histology by Ranvier in 1887. Its use in TEM as a fixative or stain was reported by Gaylarde et al. However,the preservation of the cellular ultrastructure was very poor after RuO4 fixation alone, because RuO4 is a far more vigorous oxidant than osmium tetroxide. Peltari et al reported that a preceeding glutaraldehyde fixation helped to stabilize the ultrastructure, although the penetration of RuO4 into the tissue was poor.In the present study, RuO4 has been found to be a useful fixative and staining reagent with the prerequisite of using RuO4 as a postfixative after prefixation with tannic acid (TA)-glutaraldehyde(GL) for thin-section TEM(TA-RuO4 method) or after preceeding osmication followed by TA mordanting for non-coating SEM(OsO4-TA-RuO4 method).TA-RuO4 method for thin-section TEM

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Correlation between movement of concanavalin A membrane receptors and cytolysis. A scanning electron microscopy study.

The Journal of Cell Biology ◽

10.1083/jcb.75.2.388 ◽

1977 ◽

Vol 75 (2) ◽

pp. 388-397 ◽

Cited By ~ 7

Author(s):

S Lustig ◽

O Ascher ◽

P Fishman ◽

M Djaldetti ◽

D H Pluznik

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Concanavalin A ◽

Microscopy Study ◽

Electron Microscopy Study ◽

Membrane Receptors ◽

Lateral Mobility ◽

Con A ◽

Lectin Receptor ◽

Scanning Electron

The present study was undertaken to test whether cytolysis induced by Concanavalin A (Con A) requires lateral mobility of membranal lectin receptor sites into caps. Treatment of interphase murine mastocytoma cells with 10(-4) M colchicine promoted cap formation by Con A in about 30% of the cells, followed by cytolysis. Pretreatment of the cells with NaN3, low temperature, or glutaraldehyde decreased the degree of capping and, to the same extent, the degree of cytolysis. The addition of antibodies to cells bound with Con A increased the appearance of capping and cytolysis. A linear relationship with a high correlation coefficient exists between the degree of capping and cytolysis, suggesting that lateral mobility of membrane Con A receptors is required for cytolysis by the lectin. The process of cap formation by Con A up to the stage of cytolysis was followed by scanning electron microscopy.

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