Deoxyribonucleic Acid Flow Cytometry of Benign Prostatic Disease

1989 ◽  
Vol 142 (3) ◽  
pp. 759-762 ◽  
Author(s):  
Arline D. Deitch ◽  
Melanie A. Strand ◽  
Ralph W. deVere White
Keyword(s):  
Flow Cytometry ◽  
Deoxyribonucleic Acid ◽  
Prostatic Disease

Prognostic Significance of Flow Cytometry Deoxyribonucleic Acid Analysis of Human Oligodendrogliomas

Neurosurgery ◽  
1994 ◽  
Vol 34 (4) ◽  
pp. 680-687 ◽  
Author(s):  
Stephen W. Coons ◽  
Peter C. Johnson ◽  
Dennis K. Pearl ◽  
Astrid G. Olafsen
Keyword(s):  
Flow Cytometry ◽  
Deoxyribonucleic Acid ◽  
Prognostic Significance

Prediction of Drug Cytotoxicity in 9L Rat Brain Tumor by Using Flow Cytometry with a Deoxyribonucleic Acid-binding Dye

Neurosurgery ◽  
1997 ◽  
Vol 40 (4) ◽  
pp. 782-788 ◽  
Author(s):  
Yasuo Iwadate ◽  
Shuichi Fujimoto ◽  
Kanji Sueyoshi ◽  
Hiroki Namba ◽  
Masatoshi Tagawa ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Brain Tumor ◽  
Rat Brain ◽  
Deoxyribonucleic Acid ◽  
Rat Brain Tumor

scholarly journals Flow cytometric analysis of bromodeoxyuridine-substituted cells stained with 33258 Hoechst.

1977 ◽  
Vol 25 (7) ◽  
pp. 927-934 ◽  
Author(s):  
S A Latt ◽  
Y S George ◽  
J W Gray
Keyword(s):  
Flow Cytometry ◽  
Cell Growth ◽  
Deoxyribonucleic Acid ◽  
Flow Cytometric Analysis ◽  
Chinese Hamster ◽  
Acid Synthesis ◽  
Whole Cells ◽  
Flow Cytometric ◽  
Deoxyribonucleic Acid Synthesis ◽  
Average Fluorescence

This paper describes a flow-cytometric application of the quenching of fluorescence from 33258 Hoechst stained Chinese hamster ovary-line cells due to the incorporation of 5-bromo-deoxyuridine (BrdU) into the cellular deoxyribonucleic acid. Cells were grown for 24 hr in medium containing BrdU in concentrations ranging from 1 x 10(-8) to 1 x 10(-4) M. For each concentration we measured the average fluorescence as determined by flow cytometry, the extent of BrdU substitution and the effect of the BrdU on cell growth. We determined that a BrdU concentration of 1 x 10(-5) M resulted in sufficient substitution to quench the fluorescence from 33258 Hoechst by a factor of 4, allowing discrimination between cycling and noncycling cells. The extent of BrdU substitution after growth for 24 hr in this concentration of BrdU was 64%. These data indicate the feasibility of detecting deoxyribonucleic acid synthesis in whole cells using the 33258 Hoechst-BrdU methodology.

Deoxyribonucleic acid flow cytometry and semiquantitative histology of spermatogenesis: a comparative study

1992 ◽  
Vol 58 (5) ◽  
pp. 1040-1045 ◽  
Author(s):  
Anton Hittmair ◽  
Hermann Rogatsch ◽  
Felix Offner ◽  
Hans Feichtinger ◽  
Dietmar Öfner ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Comparative Study ◽  
Deoxyribonucleic Acid

Diagnostic Accuracy of Urinary Cytology, and Deoxyribonucleic Acid Flow Cytometry and Cytology on Bladder Washings During Followup for Bladder Tumors

1997 ◽  
Vol 157 (5) ◽  
pp. 1660-1664 ◽  
Author(s):  
Mireille Gregoire ◽  
Yves Fradet ◽  
Francois Meyer ◽  
Bernard Tetu ◽  
Richard Bois ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Diagnostic Accuracy ◽  
Deoxyribonucleic Acid ◽  
Urinary Cytology ◽  
Bladder Tumors
Sign in / Sign up

Export Citation Format

Share Document