The protective role of NF-κB and AP-1 in arsenite-induced apoptosis in aortic endothelial cells

2003 ◽  
Vol 191 (2) ◽  
pp. 177-187 ◽  
Author(s):  
Tsui-Chun Tsou ◽  
Feng-Yuan Tsai ◽  
Meng Chan Wu ◽  
Louis W Chang
Keyword(s):  
Endothelial Cells ◽  
Protective Role ◽  
Aortic Endothelial Cells ◽  
Induced Apoptosis ◽  
Aortic Endothelial

Functional expression of Kir2.x in human aortic endothelial cells: the dominant role of Kir2.2

2005 ◽  
Vol 289 (5) ◽  
pp. C1134-C1144 ◽  
Author(s):  
Yun Fang ◽  
Gernot Schram ◽  
Victor G. Romanenko ◽  
Congzhu Shi ◽  
Lisa Conti ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Dominant Role ◽  
Functional Expression ◽  
Inward Rectifier ◽  
Dominant Negative ◽  
Current Sensitivity ◽  
Aortic Endothelial Cells ◽  
Human Aortic Endothelial Cells ◽  
Aortic Endothelial

Inward rectifier K+ channels (Kir) are a significant determinant of endothelial cell (EC) membrane potential, which plays an important role in endothelium-dependent vasodilatation. In the present study, several complementary strategies were applied to determine the Kir2 subunit composition of human aortic endothelial cells (HAECs). Expression levels of Kir2.1, Kir2.2, and Kir2.4 mRNA were similar, whereas Kir2.3 mRNA expression was significantly weaker. Western blot analysis showed clear Kir2.1 and Kir2.2 protein expression, but Kir2.3 protein was undetectable. Functional analysis of endothelial inward rectifier K+ current ( IK) demonstrated that 1) IK current sensitivity to Ba2+ and pH were consistent with currents determined using Kir2.1 and Kir2.2 but not Kir2.3 and Kir2.4, and 2) unitary conductance distributions showed two prominent peaks corresponding to known unitary conductances of Kir2.1 and Kir2.2 channels with a ratio of ∼4:6. When HAECs were transfected with dominant-negative (dn)Kir2.x mutants, endogenous current was reduced ∼50% by dnKir2.1 and ∼85% by dnKir2.2, whereas no significant effect was observed with dnKir2.3 or dnKir2.4. These studies suggest that Kir2.2 and Kir2.1 are primary determinants of endogenous K+ conductance in HAECs under resting conditions and that Kir2.2 provides the dominant conductance in these cells.

Role of Flavoproteins in Mediating Cellular Responses to 15-deoxy-Δ12,14-prostaglandin J2 in Bovine Aortic Endothelial Cells

2010 ◽  
Vol 49 ◽  
pp. S137
Author(s):  
Stephanie Brook Wall ◽  
Karina C. Ricart ◽  
Fen Zhou ◽  
Michelle S. Johnson ◽  
Praveen Vayalil Kumar ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Cellular Responses ◽  
Aortic Endothelial Cells ◽  
Bovine Aortic Endothelial Cells ◽  
Prostaglandin J2 ◽  
Aortic Endothelial

scholarly journals Chronic hypertension increases aortic endothelial hydraulic conductivity by upregulating endothelial aquaporin-1 expression

2017 ◽  
Vol 313 (5) ◽  
pp. H1063-H1073 ◽  
Author(s):  
Jimmy Toussaint ◽  
Chirag Bharavi Raval ◽  
Tieuvi Nguyen ◽  
Hadi Fadaifard ◽  
Shripad Joshi ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Hydraulic Conductivity ◽  
Water Transport ◽  
Aortic Endothelial Cells ◽  
Rat Models ◽  
Aquaporin 1 ◽  
Hypertensive Rat ◽  
And Function ◽  
Aortic Endothelial

Numerous studies have examined the role of aquaporins in osmotic water transport in various systems, but virtually none have focused on the role of aquaporin in hydrostatically driven water transport involving mammalian cells save for our laboratory’s recent study of aortic endothelial cells. Here, we investigated aquaporin-1 expression and function in the aortic endothelium in two high-renin rat models of hypertension, the spontaneously hypertensive genetically altered Wistar-Kyoto rat variant and Sprague-Dawley rats made hypertensive by two-kidney, one-clip Goldblatt surgery. We measured aquaporin-1 expression in aortic endothelial cells from whole rat aortas by quantitative immunohistochemistry and function by measuring the pressure-driven hydraulic conductivities of excised rat aortas with both intact and denuded endothelia on the same vessel. We used them to calculate the effective intimal hydraulic conductivity, which is a combination of endothelial and subendothelial components. We observed well-correlated enhancements in aquaporin-1 expression and function in both hypertensive rat models as well as in aortas from normotensive rats whose expression was upregulated by 2 h of forskolin treatment. Upregulated aquaporin-1 expression and function may be a response to hypertension that critically determines conduit artery vessel wall viability and long-term susceptibility to atherosclerosis. NEW & NOTEWORTHY The aortic endothelia of two high-renin hypertensive rat models express greater than two times the aquaporin-1 and, at low pressures, have greater than two times the endothelial hydraulic conductivity of normotensive rats. Data are consistent with theory predicting that higher endothelial aquaporin-1 expression raises the critical pressure for subendothelial intima compression and for artery wall hydraulic conductivity to drop.

scholarly journals Ehrlichia ruminantium uses its transmembrane protein Ape to adhere to host bovine aortic endothelial cells

2021 ◽  
Author(s):  
Valerie Pinarello ◽  
Elena Bencurova ◽  
Isabel Marcelino ◽  
Olivier Gros ◽  
Carinne Puech ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Host Cell ◽  
Transmembrane Protein ◽  
Host Cells ◽  
Ehrlichia Ruminantium ◽  
Aortic Endothelial Cells ◽  
Bovine Aortic Endothelial Cells ◽  
Early Interaction ◽  
Aortic Endothelial

Ehrlichia ruminantium is an obligate intracellular bacterium, transmitted by ticks of the genus Amblyomma and responsible for heartwater, a disease of domestic and wild ruminants. High genetic diversity of E. ruminantium strains hampers the development of an effective vaccine against all strains present in the field. In order to develop strategies for the control of heartwater through both vaccine and alternative therapeutic approaches, it is important to first gain a better understanding of the early interaction of E. ruminantium and its host cell. Particularly, the mechanisms associated with bacterial adhesion remain to elucidate. Herein, we studied the role of E. ruminantium membrane protein ERGA_CDS_01230 (UniProt Q5FFA9), a probable iron transporter, in the adhesion process to host bovine aortic endothelial cells (BAEC). The recombinant version of the protein ERGA_CDS_01230, successfully produced in the Leishmania tarentolae system, is O-glycosylated. Following in vitro culture of E. ruminantium in BAEC, the expression of CDS ERGA_CDS_01230 peaks at the extracellular infectious elementary body stages. This result suggest the likely involvement of ERGA_CDS_01230, named hereafter Ape for Adhesion protein of Ehrlichia, in the early interaction of E. ruminantium with its host cells. We showed using flow cytometry and scanning electron microscopy that beads coated with recombinant ERGA_CDS_01230 (rApe) adheres to BAEC. In addition, we also abserved that rApe interacts with proteins of the cell lysate, membrane and organelle fractions. Additionally, enzymatic treatment degrading dermatan and chondroitin sulfates on the surface of BAEC is associated with a 50% reduction in the number of bacteria in the host cell after a development cycle, indicating that glycosaminoglycans seem to play a role in the adhesion of E. ruminantium to the host cell. Finally, Ape induces a humoral response in vaccinated animals. Globally, our work identifying the role of Ape in E. ruminantium adhesion to host cells makes it a gold vaccine candidate and represents a first step toward the understanding of the mechanisms of cell invasion by E. ruminantium.

scholarly journals The Role Of Autophagy In Angiogenesis In Aortic Endothelial Cells

2010 ◽  
Vol 24 (S1) ◽  
Author(s):  
Jianhai Du ◽  
Ru‐Jeng Teng ◽  
Tongju Guan ◽  
Hao Xu ◽  
Kirkwood A Prichard ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Aortic Endothelial Cells ◽  
Aortic Endothelial
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