A Novel Notion of Local and Nonlocal Deformation-Gamuts to Model Elastoplastic Deformation

Localization and nonlocalization are characterized as a measure of degrees of separation between two material points in material’s discrete framework and as a measure of unshared and shared information, respectively, manifested as physical quantities between them, in the material’s continuous domain. A novel equation of motion to model the deformation dynamics of material is proposed. The shared information between two localizations is quantified as nonlocalization via a novel multiscale notion of Local and Nonlocal Deformation-Gamuts or DG Localization and Nonlocalization. Its applicability in continuum mechanics to model elastoplastic deformation is demonstrated. It is shown that the stress–strain curves obtained using local and nonlocal deformation-gamuts are found to be in good agreement with the Ramberg–Osgood equation for the material considered. It is also demonstrated that the cyclic strain hardening exponent and cyclic stress–strain coefficient computed using local and nonlocal deformation-gamuts are comparable with the experimental results as well as the theoretical estimations published in the open literature.

Lamin A/C-Dependent Translocation of Megakaryoblastic Leukemia-1 and β-Catenin in Cyclic Strain-Induced Osteogenesis

Lamins are intermediate filaments that play a crucial role in sensing mechanical strain in the nucleus of cells. β-catenin and megakaryoblastic leukemia-1 (MKL1) are critical signaling molecules that need to be translocated to the nucleus for their transcription in response to mechanical strain that induces osteogenesis. However, the exact molecular mechanism behind the translocation of these molecules has not been fully investigated. This study used 10% cyclic strain to induce osteogenesis in the murine osteoblast precursor cell line (MC3T3). The translocation of β-catenin and MKL1 was studied by performing knockdown and overexpression of lamin A/C (LMNA). Cyclic strain increased the expression of osteogenic markers such as alkaline phosphatase (ALP), runt-related transcription factor 2 (RUNX2), and enhanced ALP staining after seven days of incubation. Resultantly, MKL1 and β-catenin were translocated in the nucleus from the cytoplasm during the stress-induced osteogenic process. Knockdown of LMNA decreased the accumulation of MKL1 and β-catenin in the nucleus, whereas overexpression of LMNA increased the translocation of these molecules. In conclusion, our study indicates that both MKL1 and β-catenin molecules are dependent on the expression of LMNA during strain-induced osteogenesis.

Fatigue Tests on Buried or Repaired Dented Steel Pipeline Specimens

This paper presents the results of fatigue tests performed on dented steel pipeline specimens that were tested under different environmental conditions and subjected to cyclic internal pressure. Thirty-three pipe specimens were divided into three groups and tested under three different conditions. A first set of nine dented specimens was tested in air without any restrictions. A second set of eight specimens was tested while buried in the soil. A third set of sixteen specimens was tested in air, after the dents had been repaired by composite material sleeves. Hot-spot cyclic strain amplitudes were measured using two experimental techniques: Digital Image Correlation (DIC) and Fiber Optic Bragg Strain Gauges (FBSG). At first, all thirty-three specimens were tested in air along five full cycles in order to carry out full-field measurements using DIC to identify and quantify strain concentration at sites that were potential locations for fatigue cracks to initiate. Close to these point-locations, measurements of strains using FBSG were also made, and the results were then compared with the DIC results. FBSG were also used during the cyclic pressure loading process while the specimens were being tested, in such a way as to monitor the influence of the environment in the dented areas. The test results demonstrated that a simple uniaxial Manson-Coffin fatigue equation that uses the universal exponents proposed by Manson, together with the circumferential strain amplitude measured at the hot spots can be used to predict the fatigue life of the dented specimens. Moreover, it was determined that the measured strains at the hot-spot locations were not influenced by the soil coverage, although showing a considerable and beneficial decrease in their amplitudes caused by the composite repair reinforcements.

Enhanced assessment of technological factors for Ti-6Al-4V and Al-Cu-Mg strength properties

Introduction. The strength of construction materials when used under cyclic loads is of great importance in design engineering. A significant number of factors that affect the fatigue resistance have predetermined the creation of numerous methods that consider such influence. Nondestructive methods that are based on the connection of the physical degradation of material with strain properties enable evaluating experimentally the fatigue properties of materials. Purpose of study: the analysis of the processes of energy dissipation and strain accumulation during the inelastic cyclic strain of samples, using the VT6 (Ti-6Al-4V) titanium alloy and the D16 (Al-Cu-Mg) aluminum alloy before and after the technological impact. The work experimentally investigates the physical processes of degradation of the VT6 and D16 alloy samples that accompany the process of fatigue failure in materials with homogeneous and inhomogeneous stress-strain states in the concentrator (in the form of a hole and a weld). Typical modes are used to reach the fatigue testing that determine the critical stress in a material sample – the stress at which physical properties (temperature, strain) change without reaching the fatigue failure of samples. Critical stress amplitudes in the cycle, based on the data obtained during the experiment and the results of mathematical simulation, are compared. The effect of stress concentrators on critical loads that a detail can withstand after a unit operation is estimated by the finite-element method (FEM). As a result, the effect of the operational and technological factors on critical stress determined by strain and temperature is estimated. Comparative tests of the VT6 and D16 alloy samples with and without stress concentrators showed that the amplitudes of critical stress decrease by more than 30% in comparison with the ones that are without stress concentrators. The low-cycle fatigue tests of the D16 alloy samples are carried out. Mathematical simulation of the cyclic strain of the samples is carried out using MSC.Marc package. The results of the cyclic loading tests, which show that the characteristics of the technological process reduce the amplitudes of the critical stress of the VT6 and D16 alloys and affect the fatigue properties of the D16 aluminum alloy, are discussed. Mathematical simulation corresponded positively to the experimental data. Such correspondence indicates the possibility of conducting qualitative numerical assessments of the beginning of the inelastic strain accumulation process in structures with stress concentrators under the cyclic stress and the increasing stress amplitude, using the typical sample made of hardening elastoplastic material.

Mouse Lung Organoid Responses to Reduced, Increased, and Cyclic Stretch

Most lung development occurs in the context of cyclic stretch. Alteration of the mechanical microenvironment is a common feature of many pulmonary diseases with congenital diaphragmatic hernia (CDH) and fetal tracheal occlusion (FETO, a therapy for CDH) being extreme examples with changes in lung structure, cell differentiation and function. To address limitations in cell culture and in vivo mechanotransductive models we developed two mouse lung organoid (mLO) mechanotransductive models using postnatal day 5 (PND5) mouse lung CD326-positive cells and fibroblasts subjected to increased, decreased, and cyclic strain. In the first model, mLOs were exposed to forskolin (FSK) and/or disrupted (DIS) and evaluated at 20 hours. mLO cross-sectional area changed by +59%, +24% and -68% in FSK, control, and DIS mLOs respectively. FSK-treated organoids had twice as many proliferating cells as other organoids. In the second model, 20 hours of 10.25% biaxial cyclic strain increased the mRNAs of lung mesenchymal cell lineages compared to static stretch and no stretch. Cyclic stretch increased TGF-β and integrin-mediated signaling with upstream analysis indicating roles for histone deacetylases, microRNAs, and long non-coding RNAs. Cyclic stretch mLOs increased αSMA- and αSMA-PDGFRα-double positive cells compared to no stretch and static stretch mLOs. In this PND5 mLO mechanotransductive model, cell proliferation is increased by static stretch, and cyclic stretch induces mesenchymal gene expression changes important in postnatal lung development.

Investigation of ultrasonically welded thermoplastic composite joints using netlike energy directors

This paper proposes a netlike energy director (ED) made of carbon fiber-reinforced thermoplastic (CFRP) composites to improve the ultrasonic welding energy efficiency. To explore the benefits of using netlike EDs, the joint qualities with flat EDs and without EDs are included for references. Also, the influence of the netlike ED geometry (i.e., hexagonal nets with different side lengths and thicknesses) on weld attributes and joint quality are investigated in terms of joint cross-sectional microstructure, fracture morphology, and maximum shear load. It is found that for both the netlike and flat EDs, the welding process can be accelerated compared to the one without EDs, which is caused by the concentration of welding energy into the expected welding region and the avoidance of welding edges introduced by surface curvature. Meanwhile, compared with flat EDs, the maximum shear loads of the joints with netlike EDs are improved, introduced by the decrease of the contact area and consequent higher cyclic strain, resulting in more melted materials during the ultrasonic welding process. However, with the increasing of the netlike ED thickness, more porosities are generated in the welding layer leading to reduced bonded region and decrease of the maximum shear load. From the joint fracture morphology analysis, it is found that the netlike EDs introduces carbon fibers in the welding layer, and the fracture modes include fiber-matrix debonding and fiber pull-out in addition to polymer fracture, confirming the feasibility of improving joint quality by introducing CFRP netlike EDs.

Current strategies of mechanical stimulation for maturation of cardiac microtissues

The most advanced in vitro cardiac models are today based on the use of induced pluripotent stem cells (iPSCs); however, the maturation of cardiomyocytes (CMs) has not yet been fully achieved. Therefore, there is a rising need to move towards models capable of promoting an adult-like cardiomyocytes phenotype. Many strategies have been applied such as co-culture of cardiomyocytes, with fibroblasts and endothelial cells, or conditioning them through biochemical factors and physical stimulations. Here, we focus on mechanical stimulation as it aims to mimic the different mechanical forces that heart receives during its development and the post-natal period. We describe the current strategies and the mechanical properties necessary to promote a positive response in cardiac tissues from different cell sources, distinguishing between passive stimulation, which includes stiffness, topography and static stress and active stimulation, encompassing cyclic strain, compression or perfusion. We also highlight how mechanical stimulation is applied in disease modelling.

Effects of actuation of nanoporous gold on cell orientation in a fibroblast sheet

Mechanical stimulation such as flood flow often plays a vital role in the growth and maintenance of a living body, and it is important to investigate cell responses to mechanical stimulation. To date, cell responses to mechanical stimulation have been investigated in detail. However, the cell responses have been little known in a cell sheet. In the present study, a small cyclic strain (CS) of ~0.5% generated by a nanoporous gold actuator was loaded on a cell sheet of fibroblasts, and the effects of the CS on cell orientation were investigated. Individual cells were randomly distributed after the CS application, whereas cells were oriented in a specific direction after the CS application for the cell sheet. Thus, the CS had a different effect on the cell sheet from that on the individual cells. It is suggested that the cadherin/p-120 catenin complex played an important role in the cell response to mechanical stimulation in a cell sheet.