Reversible changes in rat spermatogenesis induced by an antifertility substance (Gossypol)

Spermatogonial stem cells (SSCs) are the foundation of spermatogenesis, and are characterised by their ability to self-renew and to produce differentiated progeny that form spermatozoa. It has been demonstrated that rat spermatogenesis can occur in the seminiferous tubules of congenitally immunodeficient recipient mice after transplantation of rat SSCs. However, the testis is often viewed as an immune-privileged site in that autoimmunogenic antigens on germ cells do not normally elicit an immune response in situ. In the present study, we tried to transplant rat SSCs into immunocompetent mice after depletion of their own germ cells by means of busulfan. The results showed that some transplanted SSCs could undergo complete spermatogenesis in recipient mouse testes, the rat spermatozoa being detected in 7 of 28 recipient epididymides. A significant increase in mouse spermatozoa was also noted in all 28 epididymides of recipient mice regardless of whether rat spermatozoa were concurrently present or not. These results suggest that transplanted rat SSCs can be tolerated in the testes of immunocompetent mice and that the transplantation of rat SSCs stimulates endogenous spermatogenesis in the recipient mice.

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Flow cytometric analysis of effects of 1,3‐dinitrobenzene on rat spermatogenesis

Journal of Toxicology and Environmental Health ◽

10.1080/15287398909531330 ◽

1989 ◽

Vol 28 (1) ◽

pp. 81-98 ◽

Cited By ~ 17

Author(s):

Donald P. Evenson ◽

Frank C. Janca ◽

Lorna K. Jost ◽

Rebecca K. Baer ◽

David S. Karabinus

Keyword(s):

Flow Cytometric Analysis ◽

Flow Cytometric ◽

Rat Spermatogenesis

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Rat in vitro spermatogenesis promoted by chemical supplementations and oxygen-tension control

Scientific Reports ◽

10.1038/s41598-021-82792-2 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Takafumi Matsumura ◽

Takuya Sato ◽

Takeru Abe ◽

Hiroyuki Sanjo ◽

Kumiko Katagiri ◽

...

Keyword(s):

Oxygen Tension ◽

Culture Medium ◽

Animal Species ◽

Culture Method ◽

Tension Control ◽

Significant Progress ◽

In Vitro Spermatogenesis ◽

Lower Oxygen ◽

Rat Spermatogenesis

AbstractIn vitro spermatogenesis (IVS) using air–liquid interphase organ culture method is possible with mouse testis tissues. The same method, however, has been hardly applicable to animals other than mice, only producing no or limited progression of spermatogenesis. In the present study, we challenged IVS of rats with modifications of culture medium, by supplementing chemical substances, including hormones, antioxidants, and lysophospholipids. In addition, reducing oxygen tension by placing tissues in an incubator of lower oxygen concentration and/or applying silicone cover ceiling on top of the tissue were effective for improving the spermatogenic efficiency. Through these modifications of the culture condition, rat spermatogenesis up to round spermatids was maintained over 70 days in the cultured tissue. Present results demonstrated a significant progress in rat IVS, revealing conditions commonly favorable for mice and rats as well as finding rat-specific optimizations. This is an important step towards successful IVS in many animal species, including humans.

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