FSH receptor mRNA is expressed stage-dependently during rat spermatogenesis

Binding sites for LH/hCG are found in the uterus of several species, including humans. In cattle and pigs, the LH receptor, its mRNA and LH receptor protein are present in the uterus throughout the oestrous cycle, and maximum expression occurs at the luteal phase. GnRH receptor is also expressed maximally in the human endometrium at the luteal phase. LH activates both the adenylate cyclase and phospholipase C pathways and increases the concentrations of cyclooxygenase and its products. Activation of LH receptors in the endometrium is associated with PGF production. In contrast, bovine uterine vein LH receptor mRNA and LH receptor concentrations are greatest during pro-oestrus-oestrus and LH increases the production of both PGE and PGF. FSH receptor and its mRNA are present in the bovine cervix and the concentrations are greatest at the time of the FSH peak value in the blood, indicating a physiological role for FSH in the relaxation and opening of the cervix. The presence of gonadotrophin and releasing factor receptors with a dynamic pattern in the endometrium, myometrium, oviduct and cervix of different species provides evidence that gonadotrophins and GnRH play a substantial role as molecular autocrine-paracrine regulators of the oestrous cycle and implantation.

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Increased ovulation rate in gilts treated with dihydrotestosterone

Reproduction ◽

10.1530/rep.0.1230527 ◽

2002 ◽

pp. 527-533 ◽

Cited By ~ 51

Author(s):

H Cardenas ◽

WF Pope ◽

Keyword(s):

Body Weight ◽

Androgen Receptor ◽

Follicular Phase ◽

Oestrous Cycle ◽

Ovulation Rate ◽

Corpora Lutea ◽

Fsh Receptor ◽

Receptor Mrna ◽

Preovulatory Follicles ◽

Early Follicular Phase

Treatment with testosterone increases ovulation rate in pigs. The present study was conducted to examine the effects of 5alpha-dihydrotestosterone (DHT), a non-aromatizable androgen receptor ligand, on ovulation rate and amounts of androgen receptor and FSH receptor mRNAs in postpubertal gilts. In Expt 1, ovulation rate in response to daily i.m. injections of 0, 6, 60 or 600 microg DHT kg(-1) body weight from day 13 of the oestrous cycle (day 0 = day 1 of oestrus) to the following oestrus increased with each dose of DHT (P < 0.05). The mean increase in number of corpora lutea ranged from approximately three to 17 over the three dosages of DHT. In Expt 2, gilts treated daily with 60 microg DHT kg(-1) body weight during the early follicular phase (from day 13 to day 16), coincident with follicular recruitment, or the late follicular phase (day 17 to oestrus), had higher (P < 0.05) rates of ovulation compared with gilts that received vehicle, and were not different from gilts treated with DHT from day 13 to oestrus. Percentage recovery of day 3 embryos was not altered when gilts were treated from day 13 to day 16 or from day 17 to oestrus; however, treatment of gilts with DHT from day 13 to oestrus decreased recovery of day 3 (Expt 1) or day 11 (Expt 2) conceptuses. Daily administration of 6 microg DHT kg(-1) body weight to gilts from day 13 of the oestrous cycle to the following oestrus (Expt 3) did not affect the relative amounts of androgen receptor mRNA, but increased (P < 0.05) the amounts of FSH receptor mRNA in preovulatory follicles as determined by RT-PCR. The results of these experiments indicate that androgens may regulate ovulation rate in gilts. One of the roles of androgens might be regulation of the amounts of FSH receptor mRNA in ovarian follicles.

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Seasonal relationships between dopamine D1 and D2 receptor and equine FSH receptor mRNA in equine ovarian epithelium

Animal Reproduction Science ◽

10.1016/j.anireprosci.2007.08.007 ◽

2008 ◽

Vol 108 (1-2) ◽

pp. 259-266 ◽

Cited By ~ 10

Author(s):

S.S. King ◽

K.L. Jones ◽

B.A. Mullenix ◽

D.T. Heath

Keyword(s):

D2 Receptor ◽

Fsh Receptor ◽

Receptor Mrna

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Effect of follitropin on adenylate cyclase activity and on FSH receptor mRNA expression during the ovarian cycle ofPodarcis sicula

Italian Journal of Zoology ◽

10.1080/11250000409356617 ◽

2004 ◽

Vol 71 (sup2) ◽

pp. 105-108

Author(s):

Lucia Borrelli ◽

Roberta de Stasio ◽

Elio Parisi ◽

Silvana Filosa

Keyword(s):

Adenylate Cyclase ◽

Mrna Expression ◽

Ovarian Cycle ◽

Cyclase Activity ◽

Adenylate Cyclase Activity ◽

Fsh Receptor ◽

Receptor Mrna

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Role of FSH, numbers of FSH receptors and testosterone in the regulation of inhibin secretion during the seasonal testicular cycle of adult rams

Reproduction ◽

10.1530/rep.0.1230269 ◽

2002 ◽

pp. 269-280 ◽

Cited By ~ 16

Author(s):

LM Sanford ◽

CA Price ◽

DG Leggee ◽

SJ Baker ◽

TA Yarney

Keyword(s):

Breeding Season ◽

Sertoli Cells ◽

Fsh Receptor ◽

Relative Importance ◽

Testosterone Secretion ◽

Receptor Mrna ◽

Testicular Recrudescence ◽

Natural Photoperiod ◽

Stimulation Of

The regulation of inhibin secretion has not been elucidated fully in male ruminants. The aim of this study was to determine the relative importance of FSH and testosterone concentrations, and FSH receptors, in the control of secretion of immunoactive inhibin in rams. In Expt 1, temporal changes in hormone concentrations and testicular FSH binding were determined for two groups of rams (n = 4) kept under opposite, alternating 4 month periods of long (16 h light:8 h dark) and short (8 h light:16 h dark) days. Testicular biopsies (1-2 g) were collected when the testes were regressed, redeveloping, redeveloped and regressing. In Expt 2, separate groups of rams (n = 4) kept under natural photoperiod (latitude 45 degrees 48 minutes N) were designated as controls or passively immunized (for 3 weeks) with sufficient oestradiol antiserum to increase testosterone secretion without altering LH and FSH; this was done when the testes were regressed (non-breeding season) and redeveloped (breeding season). In both groups of rams (Expt 1), 'seasonal' increases in FSH concentrations began a few weeks earlier than did increases in inhibin concentrations. FSH reached maximum concentrations during testicular recrudescence, whereas numbers of FSH receptors in the testis and circulatory inhibin concentrations did not reach peak values until the testes were fully developed. Numbers of FSH receptors per testis, but not FSH concentration, were positively correlated (r = 0.65) with inhibin concentrations across the four stages of the testicular cycle. Near the end of testicular recrudescence early in the breeding season (Expt 2), relatively high FSH concentration was associated with increased abundance of FSH receptor mRNA (90%) and number of receptors (45%) in the testis and increased inhibin concentrations (50%), compared with when the testes were regressed. Moderate, physiological increases in testosterone secretion in immunized rams did not affect inhibin in either season. These results indicate that: (i) FSH stimulation of immunoactive inhibin secretion by Sertoli cells as testes recrudesce is via increases in secretion (early) and cognate receptors (late); (ii) FSH upregulates the synthesis of its own receptor late in recrudescence; and (iii) the positive correlation (r = 0.70) observed between circulatory testosterone and immunoactive inhibin does not reflect a causal relationship.

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Relationship between adenylate cyclase sensitivity to follitropin and FSH receptor mRNA expression in the ovary of the lizardPodarcis sicula

Molecular Reproduction and Development ◽

10.1002/mrd.10090 ◽

2002 ◽

Vol 62 (2) ◽

pp. 210-215 ◽

Cited By ~ 1

Author(s):

Lucia Borrelli ◽

Roberta De Stasio ◽

Elio Parisi ◽

Silvana Filosa

Keyword(s):

Adenylate Cyclase ◽

Mrna Expression ◽

Fsh Receptor ◽

Receptor Mrna

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Restoration of human chorionic gonadotropin response in human myometrial smooth muscle cells by treatment with follicle-stimulating hormone (FSH): evidence for the presence of FSH receptors in human myometrium

Acta Endocrinologica ◽

10.1530/eje.0.1340225 ◽

1996 ◽

Vol 134 (2) ◽

pp. 225-231 ◽

Cited By ~ 21

Author(s):

JL Kornyei ◽

X Li ◽

ZM Lei ◽

ChV Rao

Keyword(s):

Smooth Muscle ◽

Smooth Muscle Cells ◽

Follicle Stimulating Hormone ◽

Muscle Cells ◽

Human Myometrium ◽

Fsh Receptor ◽

Mitogenic Response ◽

Receptor Mrna ◽

Rat Ovary ◽

Stimulating Hormone

Kornyei JL, Li X, Lei ZM, Rao ChV. Restoration of human chorionic gondadotropin response in human myometrial smooth muscle cells by treatment with follicle-stimulating hormone (FSH): evidence for the presence of FSH receptors in human myometrium. Eur J Endocrinol 1996;134:225–31. ISSN 0804–4643 Human myometrial smooth muscle cells contain receptors for human chorionic gonadotropin (hCG)/ luteinizing hormone (LH). Exogenous hCG and LH can cause a modest hyperplasia in myometrial smooth muscle cells in culture. This response is lost after about the third subculture of the cells. The present study investigated whether the loss of hCG response could be restored by co-culturing with human follicle stimulating hormone (FSH). The results showed that co-culturing with FSH can indeed restore a modest mitogenic response of hCG. However, FSH alone was not mitogenic. The FSH restoration of hCG response can be blocked by antibodies to FSH or hCG but not by non-specific rabbit IgG. The FSH treatment resulted in an increase of steady state levels of hCG/LH receptor mRNA and protein in myometrial smooth muscle cells. Since the FSH actions could be receptor mediated, we investigated the presence of FSH receptor mRNA transcripts and protein in freshly dispersed myometrial smooth muscle cells. Northern blotting demonstrated that myometrial smooth muscle cells, just as rat ovary, a classical target of FSH action, contain multiple FSH receptor mRNA transcripts. Western immunoblotting demonstrated that myometrial smooth muscle cells also contain a 60 kDa FSH receptor protein just as rat ovary and human granulosa cells used as positive control tissues. The immunocytochemistry also demonstrated that myometrial smooth muscle cells, as rat ovary and human granulosa cells, contain FSH receptor immunostaining. In summary, it is novel that FSH could restore the mitogenic response of hCG in human myometrial smooth muscle cells and these cells contain FSH receptors. These findings may have functional implications for direct regulation of human myometrium not only by hCG/LH but also by FSH. ChV Rao, Department of Obstetrics and Gynecology, 438 MDR Building, University of Louisville, School of Medicine, Louisville, Kentucky 40292, USA

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Developmental changes of testicular activin and FSH receptor mRNA and plasma FSH and inhibin levels in the rat

Life Sciences ◽

10.1016/0024-3205(93)90575-n ◽

1993 ◽

Vol 53 (16) ◽

pp. 1299-1307 ◽

Cited By ~ 8

Author(s):

Ikuro Ito ◽

Takashi Minegishi ◽

Yoshihisa Hasegawa ◽

Hiromitsu Shinozaki ◽

Kazuto Nakamura ◽

...

Keyword(s):

Developmental Changes ◽

Fsh Receptor ◽

Receptor Mrna

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227. FSH receptor expression in small human ovarian follicles

Reproduction Fertility and Development ◽

10.1071/srb05abs227 ◽

2005 ◽

Vol 17 (9) ◽

pp. 88

Author(s):

J. H. Quennell ◽

J. L. Stanton ◽

P. R. Hurst

Keyword(s):

Real Time ◽

Granulosa Cells ◽

Real Time Pcr ◽

In Situ Hybridisation ◽

Receptor Expression ◽

Follicle Development ◽

Fsh Receptor ◽

Relative Expression ◽

Receptor Mrna

Follicle-stimulating hormone (FSH) is pivotal in ovarian follicle development; the granulosa cells are the targets of FSH action in the ovary via FSH receptors. Granulosa cell growth and division mark initial follicle recruitment. The acquisition of FSH receptors on granulosa cells is regarded as a key event in hormone responsiveness and consequently follicle development. Due to the low abundance of FSH receptors and low expression of its mRNA it has been difficult to definitively characterise FSH receptor expression patterns. Here, localisation of FSH receptor in different follicle populations has been assessed with in situ hybridisation and real-time PCR of laser microdissected samples. We have used non-radioactive in situ hybridisation to investigate FSH receptor mRNA on a wide range of follicle stages. Biopsies from healthy fertile women (28–33 years) were frozen, embedded and cryosectioned at 10 µm. DIG-labelled RNA probes were designed to detect all splice variants. Hybridised probes were detected with NBT/BCIP in a colorimetric reaction. Secondly, follicles of different morphometric stages were isolated with a laser microscope. RNA extraction, reverse transcription and real-time PCR were used to confirm RNA presence and quantify relative expression. All follicle stages (from primordial to large antral) showed the presence of FSH receptor mRNA in their granulosa cells; sense controls were negative. Observations from real-time PCR indicate FSH receptor mRNA is present in all follicle stages observed and relative expression levels increase over early follicle development. These results challenge the existing doctrine that FSH receptor is absent in the smallest follicles. This suggests initial follicle recruitment may involve gonadotrophins. The use of sensitive molecular techniques will be crucial in elucidating this further.

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