Relationships among frozen-thawed sperm characteristics assessed via the routine semen analysis, sperm functional tests and fertility of bulls in an artificial insemination program

1997 ◽  
Vol 48 (5) ◽  
pp. 721-731 ◽  
Author(s):  
J.R. Correa ◽  
M.M. Pace ◽  
P.M. Zavos
Keyword(s):  
Artificial Insemination ◽  
Semen Analysis ◽  
Functional Tests ◽  
Sperm Characteristics

84 EVALUATION OF BULL SEMEN AT 1 H VS. 48 H OF POST-FROZEN STABILIZATION TIME

2006 ◽  
Vol 18 (2) ◽  
pp. 150
Author(s):  
G. M. Brogliatti ◽  
G. Larraburu ◽  
R. Cavia ◽  
M. E. Carini
Keyword(s):  
Liquid Nitrogen ◽  
Artificial Insemination ◽  
Semen Analysis ◽  
Computer Assisted ◽  
Stabilization Time ◽  
Bull Semen ◽  
Straight Line ◽  
Semen Extender ◽  
Lateral Head

The process of cryopreservation of bull semen in liquid nitrogen at −196°C is usually carried out after 3 to 6 h of refrigeration at 4°C post-collection. To guarantee the quality of the final product, the frozen straws are evaluated after cryopreservation. The seminal samples are usually stabilized during 48 h before being analyzed (Hafez, Reproduction and Artificial Insemination in Animals, 1989); this would retard the possible commercialization. The objective of the present study was to determine motility parameters and viability of semen doses stabilized by 1 h or more than 48 h in liquid nitrogen at −196°C. A total of 122 ejaculated from 23 different adult bulls (Angus, Brangus, Braford, and Hereford) were evaluated in an artificial insemination center between January and April 2005. The semen was diluted in a semi-defined semen extender (Andromed, Minitub, Germany) and frozen in an automatic freezer (Digicool, IMV, France). Parameters of velocity average path (VAP, μm/s), velocity straight line (VSL, µm/s), amplitude lateral head (ALH, µm), linearity (LIN, %), percentage of rapid cells (RAPID, %), and viability (VIA, %) were determined by Computer Assisted Semen Analysis (CASA, HTM-ceros 12.1, Berkeley, CA, USA). The obtained results were analyzed statistically with T Student and are summarized in Table 1. The results indicate that there is no difference in the velocity of the spermatozoa evaluated 1 h or 48 h post-frozen. There is no difference in VAP, VSL, movement of amplitude lateral head (ALH), or linearity (LIN). The percentage of viable spermatozoa was not affected in either group. Statistical analysis indicates that there is no difference (P > 0.05) in any of the evaluated parameters. The results demonstrate that spermatic motility and viability of frozen bull semen could be evaluated before 48 h post-frozen. This allows reduction of the time between freezing and evaluation and immediate availability of the bull straws. Table 1. Parameters of motility and viability at 1 h vs. 48 h of post-frozen stabilization time This research was supported by Centro Genético Bovino EOLIA S.A.

Semen analysis and successful artificial insemination in the St. Vincent amazon (Amazona guildingii)

2020 ◽  
Vol 148 ◽  
pp. 132-139
Author(s):  
D. Fischer ◽  
H. Schneider ◽  
S. Meinecke-Tillmann ◽  
A. Wehrend ◽  
M. Lierz
Keyword(s):  
Artificial Insemination ◽  
Semen Analysis

scholarly journals Para-chloroamphetamine (PCA)-induced ejaculation in aged rats, semen analysis and artificial insemination

1996 ◽  
Vol 30 (4) ◽  
pp. 332-336 ◽  
Author(s):  
Toru R. Saito ◽  
Ryoji Hokao ◽  
Yasumasa Wakafuji ◽  
Noriyuki Igarashi ◽  
Yoshio Agematsu ◽  
...  
Keyword(s):  
Young Adult ◽  
Sperm Motility ◽  
Artificial Insemination ◽  
Semen Analysis ◽  
Active Movement ◽  
Aged Rats ◽  
Adult Rats ◽  
Reproductive Ability ◽  
Sperm Counts ◽  
Old Rats

The present study was undertaken to determine sperm motility and counts in semen yielded by para-chloroamphetamine (PCA)-induced ejaculation of aged rats which had lost their reproductive ability, and to attempt artificial insemination with suspensions of spermatozoa obtained in this way. The semen yielded by PCA-induced ejaculation from aged (75-week-old) rats had average sperm counts of 0.82±0.69 × 107, which were much lower than the average counts (9.42±1.65 × 107) for semen spontaneously ejaculated by young adult rats (14 weeks old). However, 77.5% of the spermatozoa contained in the PCA-induced semen were rated as showing the most active movement. Spermatozoa collected in this way were injected into the upper parts of both uterine horns or into both ovarian bursae. Both methods made the females pregnant, but the results were better after injection into the ovarian bursae. The offspring born to these females showed no abnormalities.

Relationship between in vitro sperm functional tests and in vivo fertility of rams following cervical artificial insemination of ewes with frozen-thawed semen

2008 ◽  
Vol 69 (4) ◽  
pp. 513-522 ◽  
Author(s):  
C.M. O’ Meara ◽  
J.P. Hanrahan ◽  
N.S. Prathalingam ◽  
J.S. Owen ◽  
A. Donovan ◽  
...  
Keyword(s):  
Artificial Insemination ◽  
Functional Tests

scholarly journals Ultrasound Evaluation for Varicocele and its Relationship with Sperm Characteristics through Semen Analysis

2020 ◽  
Vol 7 (2) ◽  
pp. 263-267
Author(s):  
Ikhlas O. Saeed ◽  
Ibrahim A. Awad ◽  
Hamad Elniel H. Eltyib
Keyword(s):  
Positive Impact ◽  
Sperm Count ◽  
Semen Analysis ◽  
Reproductive Organs ◽  
University Hospital ◽  
Radiology Department ◽  
Sperm Characteristics ◽  
Ultrasound Evaluation ◽  
The Individual ◽  
Correct Path

The safety of the reproductive organs has a great positive impact on the human psyche, so early and accurate diagnosis of scrotal disorders by ultrasound was one of the necessities that puts the individual in the correct path of treatment. This study was conducted to evaluate the significance of ultrasound imaging technology in the diagnosis of varicocele and its relationship with sperm characteristics through semen analysis. We retrospectively reviewed a total of 281 male patients who underwent scrotum ultrasound at King Abdul-Aziz University Hospital-Radiology Department between 2017 and 2019 with age range of (11-76) years. Ultrasound result showed 119 (42.3%) had varicocele. Semen analysis was performed for 143 patients and the results showed a significant correlation between sperm characteristics and varicocele disorder (p < 0.001). Imaging of the scrotum by ultrasound is of great importance in diagnosing various andrology diseases, including varicocele, which have clear effects on sperm count and motility.

scholarly journals 7 HETEROSPERMIC INSEMINATION AT TWO SPERM CONCENTRATIONS IN TIMED AI: CASA SEMEN PARAMETERS AND PREGNANCY RATES

2005 ◽  
Vol 17 (2) ◽  
pp. 154 ◽  
Author(s):  
A. Albrecht ◽  
R. Cavia ◽  
G. Larraburu ◽  
F. Garcia Migliaro ◽  
G. Brogliatti
Keyword(s):  
Artificial Insemination ◽  
Semen Analysis ◽  
Pregnancy Rates ◽  
Semen Parameters ◽  
Computer Assisted ◽  
High Concentration ◽  
Bull Semen ◽  
Motile Cells ◽  
Timed Artificial Insemination ◽  
Motility Parameters

The latest entry in the field of semen evaluation is computer assisted semen analysis (CASA). Heterospermic insemination has been used to increase pregnancy rates from low fertile bulls. The objective of this study was to evaluate, with the aid of CASA, heterospermic semen characteristics and pregnancy rates using different concentrations of bull semen in a timed artificial insemination protocol. Semen was collected from two bulls of known fertility by artificial vagina and all CASA motility parameters were evaluated individually and combined. Straws were filled using a semi-defined semen extender (Andromed, Minitüb, Tiefenbach, Germany) as follows: single bull A and single bull B (12 × 106 of progressive motile cells after thawing); Mixed bull semen: A + B (12 × 106 of progressive motile cells after thawing) and Supermix bull semen: A + B (28 × 106 of progressive motile cells after thawing). All cows received a P4 intravaginal device (DIB, Syntex, Argentina) and 2 mg of EB i.m. (Syntex) on Day 0, 500 mg cloprostenol (Estroplan, Syntex) at the time of DIB removal (Day 8), and 1 mg EB i.m. on Day 9. Fixed-time insemination (FTAI) was performed at 52 to 56 h after DIB removal. A total of 249 cows were randomly allocated to be inseminated with bulls A and B (n = 76), with Mixed A + B (n = 87), and with Supermixed A+B at a high concentration (n = 86) by a single inseminator. Pregnancy rates were evaluated at 38 days after insemination by transrectal ultrasonography. Means and standard deviations or each characteristic were calculated, compared, and statistically analyzed. The following sperm motility parameters were determined with the Ceros 12.1 sperm analyzer (Hamilton Thorne Biosciences, Inc., Beverly, MA, USA) on at least 1000 spermatozoa: velocity average path (VAP), velocity straight line (VSL), curvilinear velocity (VCL), amplitude lateral head (ALH), beat cross frequency (BCF), straightness (STR), linearity (LIN), and percentage of rapid or static cells. There were no significant differences (P > 0.05) in VAP, VSL, VCL, ALH, STR, or LIN. There was a numerically higher percentage of rapid cells in the Supermix semen. Pregnancy rate from bulls A and B was 61% and from Mixed A + B 60%, while that from Supermixed A + B was 69%. Results from the analysis indicate that semen concentration is an important element to be considered in a timed artificial insemination program. Numerically higher pregnancy rates were obtained with double semen concentration in the straw. More research is required to evaluate the interaction between different breeds within a timed artificial insemination program. This research was supported by Centro Genético Bovino de EOLIA sa and Syntex sa Argentina.

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