New methods for microbial contamination monitoring: An experiment on board the mir orbital station

A new technology was developed for manufacturing of precise and low-cost X-ray grazing incidence microscopic optics.Imaging experiments represent one of the main directions in the Czechoslovak X-ray astronomy program. The Space Research Department of the Astronomical Institute of the Czechoslovak Academy of Sciences in Ondřejov has been active in the development of X-ray mirrors since 1970. We have participated in 7 space X-ray imaging experiments. 6 experiments were flown onboard the Vertical 8, 9 and 11 rockets in the years 1979, 1981 and 1983 (Hudec et al. 1984a), one experiment was flown onboard the Soviet orbital station Salyut 7 in the year 1982 (Valníček et al. 1983 and Hudec et al. 1984b). The experiments represent both small solar X-ray imaging telescopes and big stellar X-ray telescope.

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Contamination of Trypan Blue With Burkholderia cepacia in a Cornea Bank

Infection Control and Hospital Epidemiology ◽

10.1086/502184 ◽

2003 ◽

Vol 24 (3) ◽

pp. 198-201 ◽

Cited By ~ 6

Author(s):

Pascal C. Morel ◽

Nora Roubi ◽

Daniel R. Talon ◽

Xavier Bertrand

Keyword(s):

Molecular Typing ◽

Gel Electrophoresis ◽

Burkholderia Cepacia ◽

Trypan Blue ◽

Microbial Contamination ◽

Epidemic Strain ◽

Environmental Investigation ◽

New Methods ◽

Source Of Infection ◽

Routine Surveillance

AbstractObjective:To describe Burkholderia cepacia contamination of a cornea bank and the measures taken to identify and eliminate the source of infection.Methods:Cultures were performed to assess the extent and source of contamination, and pulsed-fleld gel electrophoresis was used for molecular typing.Results:Routine surveillance cultures identified 5 contaminated corneas during a 10-day period. Additional cultures showed that 28 of 88 samples were positive for this organism. Environmental investigation showed that an open bottle of trypan blue used to assess corneal morphology was contaminated with the epidemic strain.Conclusion:Trypan blue played a major role in this contamination of corneas. This episode shows that microbial contamination can affect transplanted corneas despite ongoing culture surveillance and suggests that new methods may be needed to avoid this risk.

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Replication of Wet Objects and Cells

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100050524 ◽

1974 ◽

Vol 32 ◽

pp. 102-103

Author(s):

S. Basu ◽

D. F. Parsons

Keyword(s):

Water Vapor Pressure ◽

High Vacuum ◽

Polystyrene Latex ◽

Vacuum System ◽

Saturated Water Vapor ◽

Replication Method ◽

New Methods ◽

Saturated Water ◽

Water Vapors ◽

Intermediate Chamber

We are approaching the invasiveness of cancer cells from the studies of their wet surface morphology which should distinguish them from their normal counterparts. In this report attempts have been made to provide physical basis and background work to a wet replication method with a differentially pumped hydration chamber (Fig. 1) (1,2), to apply this knowledge for obtaining replica of some specimens of known features (e.g. polystyrene latex) and finally to realize more specific problems and to improvize new methods and instrumentation for their rectification. In principle, the evaporant molecules penetrate through a pair of apertures (250, 350μ), through water vapors and is, then, deposited on the specimen. An intermediate chamber between the apertures is pumped independently of the high vacuum system. The size of the apertures is sufficiently small so that full saturated water vapor pressure is maintained near the specimen.

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A Freeze Shadow Technique for the Preparation of Soft Paint Latexes for Electron Microscopy

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100079097 ◽

1977 ◽

Vol 35 ◽

pp. 314-315

Author(s):

Earl R. Walter ◽

Glen H. Bryant

Keyword(s):

Sample Preparation ◽

High Vacuum ◽

Vacuum System ◽

Latex Particles ◽

New Methods ◽

Diffusion Pump ◽

Film Forming ◽

Routine Basis ◽

Distribution Studies ◽

Preparation Techniques

With the development of soft, film forming latexes for use in paints and other coatings applications, it became desirable to develop new methods of sample preparation for latex particle size distribution studies with the electron microscope. Conventional latex sample preparation techniques were inadequate due to the pronounced tendency of these new soft latex particles to distort, flatten and fuse on the substrate when they dried. In order to avoid these complications and obtain electron micrographs of undistorted latex particles of soft resins, a freeze-dry, cold shadowing technique was developed. The method has now been used in our laboratory on a routine basis for several years.The cold shadowing is done in a specially constructed vacuum system, having a conventional mechanical fore pump and oil diffusion pump supplying vacuum. The system incorporates bellows type high vacuum valves to permit a prepump cycle and opening of the shadowing chamber without shutting down the oil diffusion pump. A baffeled sorption trap isolates the shadowing chamber from the pumps.

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Directional observation on lipid of male nectary of Lindera megaphylla hemsl. by the rapid embedding method with polyethylene glycol (PEG)

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100161151 ◽

1990 ◽

Vol 48 (3) ◽

pp. 718-719

Author(s):

Dai Dalin ◽

Guo Jianmin

Keyword(s):

Electron Microscope ◽

Polyethylene Glycol ◽

Chemical Reaction ◽

Traditional Method ◽

Osmium Tetroxide ◽

Unsaturated Lipid ◽

Embedding Method ◽

Long Period ◽

New Methods

Lipid cytochemistry has not yet advanced far at the EM level. A major problem has been the loss of lipid during dehydration and embedding. Although the adoption of glutaraldehyde and osmium tetroxide accelerate the chemical reaction of lipid and osmium tetroxide can react on the double bouds of unsaturated lipid to from the osmium black, osmium tetroxide can be reduced in saturated lipid and subsequently some of unsaturated lipid are lost during dehydration. In order to reduce the loss of lipid by traditional method, some researchers adopted a few new methods, such as the change of embedding procedure and the adoption of new embedding media, to solve the problem. In a sense, these new methods are effective. They, however, usually require a long period of preparation. In this paper, we do research on the fiora nectary strucure of lauraceae by the rapid-embedding method wwith PEG under electron microscope and attempt to find a better method to solve the problem mentioned above.

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Confronting the grey zone after severe brain injury

Emerging Topics in Life Sciences ◽

10.1042/etls20190115 ◽

2019 ◽

Vol 3 (6) ◽

pp. 707-711 ◽

Cited By ~ 1

Author(s):

Andrew Peterson ◽

Adrian M. Owen

Keyword(s):

Brain Injury ◽

Ethical Issues ◽

Vegetative State ◽

Clinical Care ◽

Grey Zone ◽

Severe Brain Injury ◽

New Methods ◽

Legal Decision Making ◽

Technological Developments ◽

The Brain

In recent years, rapid technological developments in the field of neuroimaging have provided several new methods for revealing thoughts, actions and intentions based solely on the pattern of activity that is observed in the brain. In specialized centres, these methods are now being employed routinely to assess residual cognition, detect consciousness and even communicate with some behaviorally non-responsive patients who clinically appear to be comatose or in a vegetative state. In this article, we consider some of the ethical issues raised by these developments and the profound implications they have for clinical care, diagnosis, prognosis and medical-legal decision-making after severe brain injury.

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