Contamination of Trypan Blue With Burkholderia cepacia in a Cornea Bank

2003 ◽  
Vol 24 (3) ◽  
pp. 198-201 ◽  
Author(s):  
Pascal C. Morel ◽  
Nora Roubi ◽  
Daniel R. Talon ◽  
Xavier Bertrand
Keyword(s):  
Molecular Typing ◽  
Gel Electrophoresis ◽  
Burkholderia Cepacia ◽  
Trypan Blue ◽  
Microbial Contamination ◽  
Epidemic Strain ◽  
Environmental Investigation ◽  
New Methods ◽  
Source Of Infection ◽  
Routine Surveillance

AbstractObjective:To describe Burkholderia cepacia contamination of a cornea bank and the measures taken to identify and eliminate the source of infection.Methods:Cultures were performed to assess the extent and source of contamination, and pulsed-fleld gel electrophoresis was used for molecular typing.Results:Routine surveillance cultures identified 5 contaminated corneas during a 10-day period. Additional cultures showed that 28 of 88 samples were positive for this organism. Environmental investigation showed that an open bottle of trypan blue used to assess corneal morphology was contaminated with the epidemic strain.Conclusion:Trypan blue played a major role in this contamination of corneas. This episode shows that microbial contamination can affect transplanted corneas despite ongoing culture surveillance and suggests that new methods may be needed to avoid this risk.

scholarly journals Molecular Typing of Klebsiella pneumoniae Isolated from Medical Centers in Kermanshah Using Pulse Field Gel Electrophoresis

2019 ◽  
Vol In Press (In Press) ◽  
Author(s):  
Azam Elahi ◽  
Alisha Akya ◽  
Roya Chegene Lorestani ◽  
Keyghobad Ghadiri ◽  
Shokofe Baakhshii
Keyword(s):  
Klebsiella Pneumoniae ◽  
Molecular Typing ◽  
Gel Electrophoresis ◽  
Pulse Field ◽  
Pulse Field Gel Electrophoresis ◽  
Medical Centers

scholarly journals Molecular typing of Escherichia coli O157[ratio ]H7 (H−) isolates from cattle in Japan

1999 ◽  
Vol 122 (2) ◽  
pp. 337-341 ◽  
Author(s):  
M. AKIBA ◽  
T. MASUDA ◽  
T. SAMESHIMA ◽  
K. KATSUDA ◽  
M. NAKAZAWA
Keyword(s):  
Escherichia Coli ◽  
Molecular Typing ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Escherichia Coli O157 ◽  
Outbreak Strain ◽  
Pulsed Field ◽  
E Coli ◽  
Biological Methods ◽  
Pfge Profiles

A total of 77 Escherichia coli O157[ratio ]H7 (H−) isolates from cattle in Japan were investigated by molecular biological methods. Most of these isolates (43 isolates) possessed the stx2 gene, but not stx1. Fifteen bacteriophage types and 50 pulsed-field gel electrophoresis (PFGE) profiles were observed. One isolate was indistinguishable from the human outbreak strain by these methods. This indicates that cattle must be considered as a possible source of human E. coli O157[ratio ]H7 infection in Japan.

scholarly journals Whole genome sequencing provides an unambiguous link between Salmonella Dublin outbreak strain and a historical isolate

2015 ◽  
Vol 144 (3) ◽  
pp. 576-581 ◽  
Author(s):  
M. MOHAMMED ◽  
N. DELAPPE ◽  
J. O'CONNOR ◽  
P. McKEOWN ◽  
P. GARVEY ◽  
...  
Keyword(s):  
Whole Genome Sequencing ◽  
Genome Sequencing ◽  
Gel Electrophoresis ◽  
Invasive Disease ◽  
Pulsed Field Gel Electrophoresis ◽  
Whole Genome ◽  
Outbreak Strain ◽  
Salmonella Dublin ◽  
Source Of Infection ◽  
Human Salmonellosis

SUMMARYSalmonella enterica subsp. enterica serovar Dublin is an uncommon cause of human salmonellosis; however, a relatively high proportion of cases are associated with invasive disease. The serotype is associated with cattle. A geographically diffuse outbreak of S. Dublin involving nine patients occurred in Ireland in 2013. The source of infection was not identified. Typing of outbreak associated isolates by pulsed-field gel electrophoresis (PFGE) was of limited value because PFGE has limited discriminatory power for S. Dublin. Whole genome sequencing (WGS) showed conclusively that the isolates were closely related to each other, to an apparently unrelated isolate from 2011 and distinct from other isolates that were not readily distinguishable by PFGE.

scholarly journals Investigation of Melioidosis Outbreak in Pig Farms in Southern Thailand

2020 ◽  
Vol 7 (1) ◽  
pp. 9 ◽  
Author(s):  
Wiyada Kwanhian ◽  
Treenate Jiranantasak ◽  
Aleeza T. Kessler ◽  
Bryn E. Tolchinsky ◽  
Sarah Parker ◽  
...  
Keyword(s):  
Burkholderia Pseudomallei ◽  
Contaminated Water ◽  
Environmental Investigation ◽  
Source Of Infection ◽  
Local Sequence ◽  
Southern Thailand ◽  
Life Threatening ◽  
The Tropics ◽  
Privately Owned ◽  
Sequence Types

Melioidosis, caused by the Gram-negative bacterium Burkholderia pseudomallei, is a potentially life-threatening infection that can affect humans and a wide variety of animals in the tropics. In December 2017, a swine melioidosis case was discovered during a meat inspection at a privately-owned slaughterhouse in Nakhon Si Thammarat Province in southern Thailand. The infection, which continued for several months, caused a dispute about where the disease began. An environmental investigation into two farms—both involved in raising the first infected pig—ensued. Through genetic analysis, the investigation revealed that a contaminated water supply at one farm was the probable source of infection. The three local sequence types identified in the investigation were types 51, 298 and 392.

Presence of Salmonella in the Red Meat Abattoir Lairage after Routine Cleansing and Disinfection and on Carcasses

2006 ◽  
Vol 69 (10) ◽  
pp. 2342-2351 ◽  
Author(s):  
A. SMALL ◽  
C. JAMES ◽  
S. JAMES ◽  
R. DAVIES ◽  
E. LIEBANA ◽  
...  
Keyword(s):  
Foodborne Pathogens ◽  
Gel Electrophoresis ◽  
Microbial Contamination ◽  
Antimicrobial Agents ◽  
Red Meat ◽  
Pulsed Field Gel Electrophoresis ◽  
Sensitivity Testing ◽  
Beef Carcasses ◽  
Pig Carcasses ◽  
Cull Cow

Foodborne pathogens, such as Salmonella, may remain in abattoir lairages after cleansing and pose a risk of transfer and contamination from one processing day to the next. These organisms may be transferred to the outer surface of animals held in lairage facilities, and the skin or hide may be a significant source of microbial contamination on the red meat carcasses subsequently produced. Sponge samples were taken from various sites in the lairage (n = 556), and single-pass sponge samples were taken from one side of red meat carcasses (n = 1,050) at five commercial abattoirs in Southwest England and tested for the presence of Salmonella. Of these, 6.5% of lairage samples were positive, containing estimated numbers of up to 104 Salmonella organisms per sampled area (50 by 50 cm). Salmonella was found on 9.6% of 240 lamb carcasses, 12.7% of 330 beef carcasses, 31% of 70 pig carcasses, 20% of 80 calf carcasses younger than 14 days of age, and none of 330 cull cow and bull carcasses. Subtyping divided the 137 isolates into seven serogroups and three pulsed-field gel electrophoresis clusters, and sensitivity testing against a bank of 16 antimicrobials indicated that 47 isolates had resistance to one or more antimicrobial agents. These results indicate that Salmonella contamination can persist in the lairage environment from one processing day to the next and that Salmonella is present on red meat carcasses, although the implications of residual lairage contamination on carcass meat microbiology are not clear from this study. Abattoir owners should take steps to reduce the level of contamination in their premises to prevent contamination from being carried over from one processing day to the next.

scholarly journals Prevalence of SHV-12 among Clinical Isolates ofKlebsiella pneumoniae Producing Extended-Spectrum β-Lactamases and Identification of a Novel AmpC Enzyme (CMY-8) in Southern Taiwan

2000 ◽  
Vol 44 (6) ◽  
pp. 1438-1442 ◽  
Author(s):  
Jing-Jou Yan ◽  
Shiou-Mei Wu ◽  
Shu-Huei Tsai ◽  
Jiunn-Jong Wu ◽  
Ih-Jen Su
Keyword(s):  
Gel Electrophoresis ◽  
Amino Acid Sequences ◽  
Clinical Isolates ◽  
Pulse Field ◽  
Epidemic Strain ◽  
Sequence Comparisons ◽  
Pulse Field Gel Electrophoresis ◽  
Extended Spectrum ◽  
Resistance Plasmids ◽  
Southern Taiwan

ABSTRACT Twenty (8.5%) of 234 nonrepetitive clinical isolates ofKlebsiella pneumoniae from southern Taiwan were found to produce extended-spectrum β-lactamases (ESBLs): 10 strains produced SHV-12, 4 produced SHV-5, 2 produced a non-TEM non-SHV ESBL with a pI of 8.3, 3 produced a novel AmpC β-lactamase designated CMY-8 with a pI of 8.25, and 1 produced SHV-12 and an unidentified AmpC enzyme with a pI of 8.2. The CMY-8 enzyme confers a resistance phenotype similar to CMY-1 and MOX-1, and sequence comparisons showed high homologies (>95%) of nucleotide and amino acid sequences among these three enzymes. Plasmid and pulse-field gel electrophoresis analyses revealed that all isolates harboring an SHV-derived ESBL were genetically unrelated, indicating that dissemination of resistance plasmids is responsible for the spread of SHV ESBLs among K. pneumoniaein this area. All three isolates carrying CMY-8 had identical genotypic patterns, suggesting the presence of an epidemic strain.

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