Broth rheology, growth and metabolite production of Beta vulgaris suspension culture: a comparative study between cultures grown in shake flasks and in a stirred tank

1999 ◽  
Vol 24 (10) ◽  
pp. 687-693 ◽  
Author(s):  
Mario Rodrı́guez-Monroy ◽  
Enrique Galindo
Keyword(s):  
Comparative Study ◽  
Suspension Culture ◽  
Beta Vulgaris ◽  
Stirred Tank ◽  
Metabolite Production ◽  
Shake Flasks

scholarly journals Advances and Perspectives in Tissue Culture and Genetic Engineering of Cannabis

2021 ◽  
Vol 22 (11) ◽  
pp. 5671
Author(s):  
Mohsen Hesami ◽  
Austin Baiton ◽  
Milad Alizadeh ◽  
Marco Pepe ◽  
Davoud Torkamaneh ◽  
...  
Keyword(s):  
Plant Regeneration ◽  
Genetic Engineering ◽  
Suspension Culture ◽  
Secondary Metabolite ◽  
Hairy Root ◽  
Root Culture ◽  
Hairy Root Culture ◽  
Gene Transformation ◽  
Secondary Metabolite Production ◽  
Metabolite Production

For a long time, Cannabis sativa has been used for therapeutic and industrial purposes. Due to its increasing demand in medicine, recreation, and industry, there is a dire need to apply new biotechnological tools to introduce new genotypes with desirable traits and enhanced secondary metabolite production. Micropropagation, conservation, cell suspension culture, hairy root culture, polyploidy manipulation, and Agrobacterium-mediated gene transformation have been studied and used in cannabis. However, some obstacles such as the low rate of transgenic plant regeneration and low efficiency of secondary metabolite production in hairy root culture and cell suspension culture have restricted the application of these approaches in cannabis. In the current review, in vitro culture and genetic engineering methods in cannabis along with other promising techniques such as morphogenic genes, new computational approaches, clustered regularly interspaced short palindromic repeats (CRISPR), CRISPR/Cas9-equipped Agrobacterium-mediated genome editing, and hairy root culture, that can help improve gene transformation and plant regeneration, as well as enhance secondary metabolite production, have been highlighted and discussed.

scholarly journals Effect of Na-chloride on the bioleaching of a chalcopyrite concentrate in shake flasks and stirred tank bioreactors

2013 ◽  
Vol 138 ◽  
pp. 1-13 ◽  
Author(s):  
Denise Bevilaqua ◽  
Heidi Lahti ◽  
Patrícia H. Suegama ◽  
Oswaldo Garcia ◽  
Assis V. Benedetti ◽  
...  
Keyword(s):  
Stirred Tank ◽  
Shake Flasks ◽  
Chalcopyrite Concentrate

scholarly journals Handling culture medium composition for optimizing plant cell suspension culture in shake flasks

2017 ◽  
Vol 133 (1) ◽  
pp. 137-146 ◽  
Author(s):  
Tiago Fidemann ◽  
Gabriela Aparecida de Araujo Pereira ◽  
Tárik Reis Heluy ◽  
Rodrigo Boccoli Gallego ◽  
Mônica Rosa Bertão ◽  
...  
Keyword(s):  
Suspension Culture ◽  
Cell Suspension ◽  
Plant Cell ◽  
Cell Suspension Culture ◽  
Culture Medium ◽  
Medium Composition ◽  
Plant Cell Suspension Culture ◽  
Plant Cell Suspension ◽  
Shake Flasks

scholarly journals Inulinolytic activity of broths ofAspergillus nigerATCC 204447 cultivated in shake flasks and stirred tank bioreactor

2017 ◽  
Vol 17 (9) ◽  
pp. 1006-1020 ◽  
Author(s):  
Anna Kowalska ◽  
Anna Antecka ◽  
Piotr Owczarz ◽  
Marcin Bizukojć
Keyword(s):  
Stirred Tank ◽  
Stirred Tank Bioreactor ◽  
Shake Flasks
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