Comparative selectivities of immobilized lipases from Pseudomonas cepacia and Candida antarctica (fraction B) for esterification reactions with glycerol and glycerol analogues in organic media

1999 ◽  
Vol 25 (3-5) ◽  
pp. 290-297 ◽  
Author(s):  
Qing-Long Chang ◽  
Chen-Hsien Lee ◽  
Kirk L. Parkin
Keyword(s):  
Candida Antarctica ◽  
Pseudomonas Cepacia ◽  
Organic Media ◽  
Immobilized Lipases ◽  
Esterification Reactions

scholarly journals Performance of Different Immobilized Lipases in the Syntheses of Short- and Long-Chain Carboxylic Acid Esters by Esterification Reactions in Organic Media

Molecules ◽  
2018 ◽  
Vol 23 (4) ◽  
pp. 766 ◽  
Author(s):  
Lionete de Lima ◽  
Adriano Mendes ◽  
Roberto Fernandez-Lafuente ◽  
Paulo Tardioli ◽  
Raquel Giordano
Keyword(s):  
Carboxylic Acid ◽  
Organic Media ◽  
Immobilized Lipases ◽  
Esterification Reactions ◽  
Acid Esters ◽  
Long Chain

Discriminating between dispersion and lyoprotection effects in biocatalysis in organic media

2002 ◽  
Vol 80 (6) ◽  
pp. 551-554 ◽  
Author(s):  
Francesco Secundo ◽  
Giacomo Carrea ◽  
Francesco Maria Veronese
Keyword(s):  
Circular Dichroism ◽  
Ethylene Glycol ◽  
Candida Antarctica ◽  
Pseudomonas Cepacia ◽  
Organic Media ◽  
Candida Antarctica Lipase ◽  
Lipase B ◽  
Protein Ratio ◽  
Activation Effect ◽  
Circular Dichroïsm

The increment of activity and solubility in 1,4-dioxane of lipase B from Candida antarctica, lipase from Pseudomonas cepacia, and subtilisin, were investigated as a function of the methoxypoly(ethylene glycol)–protein (PEG–protein) ratio employed during lyophilization. Both activity and solubility markedly increased as the PEG–protein ratio was increased. The increment of activity at low PEG–protein ratios, however, was much higher than that of solubility. These data suggest that the PEG-induced activation effect is due mainly to a lyoprotection effect rather than to relaxation of diffusional limitations.Key words: hydrolases, activity, dispersion, lyoprotection, circular dichroism.

scholarly journals Synthesis, Biological Activity, and In silico Study of Bioesters Derived from Bixin by the CALB Enzyme

2021 ◽  
Vol 12 (5) ◽  
pp. 5901-5917
Keyword(s):  
In Silico ◽  
Biological Properties ◽  
Candida Antarctica ◽  
Candida Antarctica Lipase B ◽  
High Catalytic Activity ◽  
Candida Antarctica Lipase ◽  
Lipase B ◽  
In Silico Study ◽  
Esterification Reactions ◽  
Energy Products

Biocatalysis is a branch of biotechnology that aims at the chemical transformation of a compound by using enzymes of known specificity. There are already several studies that use combinations of organic origin and enzymes as catalysts. The enzymatic sources are diverse and can be found in microorganisms, animals, vegetables, or commercial (enzymes isolated). The enzyme Candida antarctica lipase B (CALB), of microbial origin, commercially available and with high catalytic activity, can perform esterification reactions, obtaining expressive results. These biocatalytic reactions can contribute to the development of energy products, such as biofuels and pharmaceuticals, such as cosmetics and pharmaceuticals. In this context, the present project aims to esterify with the enzyme Candida antarctica lipase B (CALB), a natural product known as bixin, a dye extracted from the pericarp of annatto seeds (Bixa orellana L.) to verify its biological properties and in silico evidence of its cholesteric activity.

A new method to determine the aw range in which immobilized lipases display optimum activity in organic media

1996 ◽  
Vol 10 (4) ◽  
Author(s):  
Miguel Arroyo ◽  
Jos�Mar�a S�nchez-Montero ◽  
Jos�Vicente Sinisterra
Keyword(s):  
New Method ◽  
Organic Media ◽  
Optimum Activity ◽  
Immobilized Lipases

Immobilization of Candida antarctica lipase B onto Purolite® MN102 and its application in solvent-free and organic media esterification

2016 ◽  
Vol 40 (1) ◽  
pp. 23-34 ◽  
Author(s):  
Marija Ćorović ◽  
Mladen Mihailović ◽  
Katarina Banjanac ◽  
Milica Carević ◽  
Ana Milivojević ◽  
...  
Keyword(s):  
Candida Antarctica ◽  
Solvent Free ◽  
Candida Antarctica Lipase B ◽  
Organic Media ◽  
Candida Antarctica Lipase ◽  
Lipase B
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