Assessment of coastal marine pollution in Galicia (NW Iberian Peninsula); metal concentrations in seawater, sediments and mussels (Mytilus galloprovincialis) versus embryo–larval bioassays using Paracentrotus lividus and Ciona intestinalis

2003 ◽  
Vol 56 (4) ◽  
pp. 531-553 ◽  
Author(s):  
R. Beiras ◽  
J. Bellas ◽  
N. Fernández ◽  
J.I. Lorenzo ◽  
A. Cobelo-Garcı́a
2021 ◽  
Vol 28 (1) ◽  
Author(s):  
Maria P. Kalaitzidou ◽  
Christina I. Nannou ◽  
Dimitra A. Lambropoulou ◽  
Konstantinos V. Papageorgiou ◽  
Alexandros M. Theodoridis ◽  
...  

Abstract Background Microcystins are emerging marine biotoxins, produced by potentially toxic cyanobacteria. Their presence has been reported in aquatic animals in Greek freshwater, while data are few in marine environments. Since the climate change induces eutrophication and harmful algal blooms in coastal marine ecosystems affecting the public health, further research on microcystins’ presence in marine waters is required. The aim of this study was to examine the potential presence of microcystins in mussels Mytilus galloprovincialis in the largest farming areas in Thermaikos gulf, in Northern Greece, and to investigate their temporal and spatial distribution, adding to the knowledge of microcystins presence in Greek Mediterranean mussels. Results A 4-year microcystins’ assessment was conducted from 2013 to 2016, in farmed Mediterranean mussels M. galloprovincialis, in five sampling areas in Thermaikos gulf, in northern Greece, where the 90% of the Greek mussels’ farming activities is located. The isolation of potentially toxic cyanobacteria was confirmed by molecular methods. An initial screening was performed with a qualitative and quantitative direct monoclonal (DM) ELISA and results above 1 ng g−1 were confirmed for the occurrence of the most common microcystins-RR, -LR and -YR, by Ultra High Performance Liquid Chromatography (UHPLC) coupled with a high- resolution mass spectrometer (HRMS) (Orbitrap analyzer). Microcystin-RR and microcystin-LR were detected, while the intensity of microcystin-YR was below the method detection limit. Most samples that exhibited concentrations above 1 ng g-1 were detected during the warm seasons of the year and especially in spring. Results indicated an overestimation of the ELISA method, since concentrations ranged between 0.70 ± 0.15 ng g−1 and 53.90 ± 3.18 ng g−1, while the confirmation denoted that the levels of microcystins were 6 to 22 times lower. Conclusions Microcystin-RR and microcystin-LR were detected for the first time in mussel M. galloprovincialis, harvested from farms in Thermaikos gulf, in Central Macedonia, Greece. Their presence was linked to potentially toxic cyanobacteria. Bioaccumulation was observed in digestive gland, while the concentrations in muscles were found extremely low. Samples with levels above 1 ng g−1 were observed mostly during spring, confirming the seasonal distribution of microcystins. The comparison of the results by the ELISA and the LC-Orbitrap MS method indicated an overestimation of concentration by the ELISA method.


2019 ◽  
Vol 35 (7) ◽  
pp. 631-643
Author(s):  
Sergey Kholodkevich ◽  
Andrey Sharov ◽  
Tatiana Kuznetsova ◽  
Anton Kurakin ◽  
Danijela Joksimović ◽  
...  

Animals ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 1739
Author(s):  
Francesco Fazio ◽  
Claudio D’Iglio ◽  
Gioele Capillo ◽  
Concetta Saoca ◽  
Katya Peycheva ◽  
...  

The environmental monitoring of chemical toxicants has been a widely studied topic in the last few decades. The main aim of the present study was to determine the total concentration of nine elements (Cd, Cr, Pb, Ni, Al, Cu, Fe, Mn, and Zn) in the fish species grey mullet (M. cephalus) and in the coastal marine waters collected from various sampling points along the Black Sea (Bulgaria) and the Ionian Sea (Italy). Further, those results were applied to predict the pollution degree in those coastal marine environments. The fish samples were subject to acid digestion followed by appropriate analytical determination. The metal concentrations in marine water samples collected from the Black Sea (Bulgaria) and the Ionian Sea (Italy) were also analyzed. Unpaired Student’s t-test and the one-way ANOVA were applied for the statistical analysis of the data. The statistical results revealed a significant variation (p < 0.0001) in the concentration of various fish tissues. The accumulation of toxic and essential elements differs significantly in grey mullet species caught from the Black Sea (Bulgaria) and the Ionian Sea (Italy). The results from this study may serve as a convenient approach during marine pollution programs set by both countries (Italy and Bulgaria).


Author(s):  
ANGELA SARDO ◽  
RACHELE ROSSI ◽  
VITTORIO SOPRANO ◽  
PATRIZIA CIMINIELLO ◽  
ERNESTO FATTORUSSO ◽  
...  

Blooms of the toxic dinoflagellates Ostreopsis have become common along rocky shores of the Mediterranean Sea. In addition to health problems for beach-goers, Ostreopsis toxins may accumulate in benthic marine animals used for human consumption, which however at times have shown signals of stress and even mortality. In order to elucidate the actual relationships between Ostreopsis and benthic invertebrates, we exposed mussels Mytilus galloprovincialis and sea urchins Paracentrotus lividus from the Gulf of Naples to cultures and natural material of O. cf. ovata and assessed feeding and adverse effects on the animals, along with their acquired toxicity. Mussels exposed to O. cf. ovata for 24 hours filtered the microalgae at different rates, depending on both mussel size and microalgal density, and became weakly toxic in some cases. Under longer exposure most animals died and all survivors were toxic. Detoxification of a naturally toxic mussel populations from an area affected by O. cf. ovata blooms took more than two weeks. Sea urchins fed with the red alga Asparagopsis taxiformis epiphytised by O. cf. ovata did not show damages and became mildly toxic in some cases. However, the direct exposure of sea urchins to O. cf. ovata cultures caused the partial or total loss of the spines in a density-dependent way, with the death of the animals at the highest microalgal concentrations. Milder effects were registered with sonicated cultures or toxin extracts. Our results indicate that the balance between toxicity and animal health in these invertebrates depends on the mode and intensity of exposure to the toxic microalga, while the response varies between the two species but also within the same species. This scenario matches the variety of responses of benthic populations recorded in the natural environment in areas affected by O. cf. ovata blooms.


Author(s):  
Norbert P. Psuty ◽  
Philip E. Steinberg

The 1990s witnessed a significant increase in popular interest in the US regarding the geography of the world’s coastal and marine spaces. Factors motivating this renewed interest included growing public environmental awareness, a decade of unusually severe coastal storms, more frequent reporting of marine pollution hazards, greater knowledge about (and technology for) depleting fishstocks, domestic legislation on coastal zone management and offshore fisheries policies, new opportunities for marine mineral extraction, heightened understanding of the role of marine life in maintaining the global ecosystem, new techniques for undertaking marine exploration, the 1994 activation of the United Nations Convention on the Law of the Sea, reauthorization of the US Coastal Zone Management Act in 1996, and designation of 1998 as the International Year of the Ocean. Responding to this situation, the breadth of perspectives from which coastal and marine issues are being encountered by geographers, the range of subjects investigated, and the number of geographers engaging in coastal-marine research have all increased during the 1990s. As West (1989a) reported in the original Geography in America, North American coastal-marine geography during the 1980s was focused toward fields such as coastal geomorphology, ports and shipping, coastal zone management, and tourism and recreation. Research in these areas has continued, but in the 1990s, with increased awareness of the importance of coastal and marine areas to physical and human systems, geographers from a range of subdisciplines beyond those usually associated with coastal-marine geography have begun turning to coastal and marine areas as fruitful sites for conducting their research. Climatologists are investigating the sea in order to understand processes such as El Niño, remote-sensing experts are studying how sonic imagery can be used for understanding species distribution in three-dimensional environments, political ecologists are investigating the ocean as a common property resource in which multiple users’ agendas portend conflict and cooperation, and cultural geographers are examining how the ocean is constructed as a distinct space with its own social meanings and “seascapes.” Despite (or perhaps because of ) this expansion in coastal-marine geography, the subdiscipline remains fragmented into what we here call “Coastal Physical Geography,” “Marine Physical Geography,” and “Coastal-Marine Human Geography.”


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