An in vitro technique for electrophysiological mapping of reptilian retinotectal projections

1998 ◽  
Vol 81 (1-2) ◽  
pp. 85-89 ◽  
Author(s):  
R.V Stirling ◽  
S.A Dunlop ◽  
L.D Beazley
Keyword(s):  
In Vitro Technique ◽  
Retinotectal Projections

An in vitro technique for surveying immunostimulants in fish

Aquaculture ◽  
1993 ◽  
Vol 112 (2-3) ◽  
pp. 283-287 ◽  
Author(s):  
Galina Jeney ◽  
Douglas P. Anderson
Keyword(s):  
In Vitro Technique

In vitro Technique for Selection of Radiation Induced Mutants of Tall Fescue

2013 ◽  
Vol 19 (1) ◽  
pp. 63-68
Author(s):  
Ki-Won Lee ◽  
Jin Young Moon ◽  
Hee Chung Ji ◽  
Gi Jun Choi ◽  
Ki-Yong Kim ◽  
...  
Keyword(s):  
Tall Fescue ◽  
In Vitro Technique ◽  
Induced Mutants ◽  
Radiation Induced ◽  
Selection Of

scholarly journals IN VITRO POTENCY AND TOXICITY OF STREPTOMYCES SP. FERMENTATION PRODUCT AS AN ANTIMALARIAL THERAPY AGAINST PLASMODIUM FALCIPARUM

2019 ◽  
pp. 251-255
Author(s):  
YUNI SETYANINGSIH ◽  
ABDUL LATIF ◽  
HENDRI ASTUTY ◽  
DIN SYAFRUDDIN ◽  
PUJI BUDI SETIA ASIH
Keyword(s):  
Plasmodium Falciparum ◽  
Inhibitory Concentration ◽  
Toxicity Tests ◽  
Streptomyces Sp ◽  
No Formation ◽  
Fermentation Product ◽  
Transmission Electron ◽  
In Vitro Technique ◽  
Antimalarial Therapy

Objective: This research aims to study the activity of a Streptomyces sp. fermentation product as an antimalarial modality in HepG2 cells.Methods: The effects of the product against Plasmodium falciparum 3D7 were examined using an in vitro technique parasite. The potency of theStreptomyces sp. fermentation product was examined by determining the half maximal inhibitory concentration (IC50), and the mechanism wasstudied using transmission electron microscopy (TEM). Toxicity tests were also conducted.Results: The Streptomyces sp. fermentation product had an IC50 of 0.001 μg/ml against the parasite, versus values of 0.054 and 0.022 μg/ml forquinidine and prodigiosin, respectively. TEM revealed no formation of hemozoin. The Streptomyces sp. fermentation product was non-toxic in HepG2cells based on its cytotoxicity concentration 50% of 1.380 μg/ml.Conclusion: The Streptomyces sp. fermentation product has potential as a potent and non-toxic antimalarial therapy.

Influence of analogues of phenylbutazone on renal transport of 4-aminohippurate

1963 ◽  
Vol 205 (3) ◽  
pp. 489-493 ◽  
Author(s):  
Agamemnon Despopoulos ◽  
Lloyd H. Pendergrass ◽  
John M. Stoeckinger
Keyword(s):  
Molecular Structure ◽  
Pharmacological Activity ◽  
Transport Mechanism ◽  
Renal Transport ◽  
Inhibitory Potency ◽  
In Vitro Technique ◽  
Dog Kidney ◽  
Relative Potencies ◽  
Clearance Technique

Analogues of phenylbutazone were tested for inhibitory potency in the renal transport mechanism for 4-aminohippurate. In a series of ten compounds examined by an in vitro technique, no correlation could be demonstrated between inhibitory potency and acidic strength of the inhibitor either in rabbit or in dog tissues. In a further series of 16 paired analogues, the hydroxylated member of each pair was 3–4 times less potent than its unhydroxylated counterpart. Correlations between molecular structure and pharmacological activity are suggested. In contrast to these observations, each of three selected phenylbutazone analogues (phenylbutazone, metabolite II, and sulfinpyrazone) depressed the maximum tubular excretory rate for 4-aminohippurate in intact dogs, but apparent inhibitory potencies by the clearance technique were unrelated to inhibitory potencies in dog kidney slices. Differences in relative potencies thus obtained are attributed to differences in metabolic alteration of each compound at extrarenal sites in intact dogs.

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