In vitro Technique for Selection of Radiation Induced Mutants of Tall Fescue

2013 ◽  
Vol 19 (1) ◽  
pp. 63-68
Author(s):  
Ki-Won Lee ◽  
Jin Young Moon ◽  
Hee Chung Ji ◽  
Gi Jun Choi ◽  
Ki-Yong Kim ◽  
...  
Author(s):  
Aleksey Sivolapov ◽  
Vladimir Sivolapov

We consider poplar selection systems in connection with their reproduction systems. Poplar is a model object on which it is possible to test all known systems for the selection of woody plants. Positive selection and synthetic selection methods (hybridization, mutagenesis, polyploidy, genetic engineering, biotechnology) effectively “work” on poplar. Poplar is propagated by seed, vegetative and in vitro biotechnology. Amphimixis or seed propagation of poplar is used to create hybrid seed plantations, for example white poplar × aspen. In natural poplar populations, spontaneous hybridization occurs, leading to the emergence of new species. The ability to vegetatively propagate poplar successfully solves the problem of plus breeding: selected best biotypes are propagated by in vitro grafting, cuttings or regenerants, and plantation cultures are created. Synthetic breeding methods: hybridization is widely used on poplar; dozens of poplar hybrids for forest crops and landscaping have been obtained; Induced mutants and polyploids are obtained. Breeders get their first genetically modified forms using genetic engineering. By microcloning, economically valuable individuals are propagated and regenerants are used to create forest crops and landscaping.


2015 ◽  
Vol 5 (4) ◽  
pp. 16-22
Author(s):  
Hong Van Doan ◽  
Ba Tuan Dinh ◽  
Tuan Anh Tran ◽  
Thuy Ngan Nguyen ◽  
Bich Thuan Ta ◽  
...  

DNA damages in Escherichia coli (E. coli) exposed to UV radiation have been investigated. After 30 min of exposure to UV radiation of 5 mJ/cm2, the growth of E. coli in LB broth medium was about only 10% in compared with non-irradiated one. This results suggested that the UV radiation caused the damages for E. coli genome resulted in reduction in its growth and survival, and those lesions can be somewhat recovered. For both solutions of plasmid DNAs and E. coli cells containing plasmid DNA, this dose also caused the breakages on single and double strands of DNA, shifted the morphology of DNA plasmid from supercoiled to circular and linear forms. The formation of pyrimidine dimers upon UV radiation significantly reduced when the DNA was irradiated in the presence of Ganoderma lucidum extract. Thus, studies on UV-induced DNA damage at molecular level are very essential to determine the UV radiation doses corresponding to the DNA damages, especially for creation and selection of useful radiation-induced mutants, as well as elucidation the protective effects of the specific compounds against UV light.


Planta Medica ◽  
2015 ◽  
Vol 81 (16) ◽  
Author(s):  
R Bertóti ◽  
Á Alberti ◽  
A Böszörményi ◽  
R Könye ◽  
T Horváth ◽  
...  

Author(s):  
B.R. Watkin

AN Aberystwyth selection of tall fescue (Festuca arundinacea Schreb.), known as S170, was sown with certified New Zealand white clover (Trifolium repens) and re' clover (T. pratense) and compared under sheep grazing with other grass/clover pastures at the Grasslands Division Regional Station at Lincoln (Watkin, 1975) .


Sign in / Sign up

Export Citation Format

Share Document