Increased Frequency of P-Glycoprotein Gene Amplification in Colchicine-Resistant Rat-1 Clones Transformed by v-src

1997 ◽  
Vol 96 (2) ◽  
pp. 157-165 ◽  
Author(s):  
Ti Lin ◽  
Jeffrey M Trent ◽  
Dina Milliken ◽  
David S Shimm ◽  
Robert Donaldson ◽  
...  
Keyword(s):  
Gene Amplification ◽  
Glycoprotein Gene ◽  
P Glycoprotein

Electrophoretic analysis of 248 clinical breast cancer specimens for P-glycoprotein overexpression or gene amplification.

1989 ◽  
Vol 7 (8) ◽  
pp. 1129-1136 ◽  
Author(s):  
D E Merkel ◽  
S A Fuqua ◽  
A K Tandon ◽  
S M Hill ◽  
A U Buzdar ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Drug Resistance ◽  
Gene Amplification ◽  
Multiple Drug Resistance ◽  
Electrophoretic Analysis ◽  
Gene Copy ◽  
Cross Resistance ◽  
Glycoprotein Gene ◽  
P Glycoprotein ◽  
Clinical Breast

Multiple drug resistance (MDR), consisting of acquired cross resistance to anthracyclines, vinca alkyloids, and other antineoplastic antibiotics, has been described in a variety of cell lines. This MDR phenotype is associated with overexpression and sometimes amplification of a gene coding for a 170 kDa glycoprotein, termed P-glycoprotein. To understand the role of this mechanism in clinical breast cancer, 248 breast cancer specimens representing both untreated primary and refractory relapsing disease were probed for evidence of P-glycoprotein gene amplification or overexpression using Southern, Northern, or Western blot techniques. In no case was an increase in P-glycoprotein gene copy number or expression detected. Though these findings do not necessarily rule out a role for P-glycoprotein in mediating drug resistance in breast cancer, electrophoretic analysis of clinical specimens is unlikely to provide useful predictive information. More sensitive assays must be developed to overcome the difficulties inherent in analyzing heterogenous tissue samples.

scholarly journals P-glycoprotein gene amplification and expression in multidrug-resistant murine P388 and B16 cell lines

1989 ◽  
Vol 59 (5) ◽  
pp. 682-685 ◽  
Author(s):  
G Capranico ◽  
P De Isabella ◽  
C Castelli ◽  
R Supino ◽  
G Parmiani ◽  
...  
Keyword(s):  
Cell Lines ◽  
Gene Amplification ◽  
Multidrug Resistant ◽  
Glycoprotein Gene ◽  
P Glycoprotein

Direct and inverted DNA repeats associated with P-glycoprotein gene amplification in drug resistant Leishmania.

1991 ◽  
Vol 10 (4) ◽  
pp. 1009-1016 ◽  
Author(s):  
M. Ouellette ◽  
E. Hettema ◽  
D. Wüst ◽  
F. Fase-Fowler ◽  
P. Borst
Keyword(s):  
Gene Amplification ◽  
Drug Resistant ◽  
Dna Repeats ◽  
Glycoprotein Gene ◽  
P Glycoprotein

scholarly journals Identification of members of the P-glycoprotein multigene family.

1989 ◽  
Vol 9 (3) ◽  
pp. 1224-1232 ◽  
Author(s):  
W F Ng ◽  
F Sarangi ◽  
R L Zastawny ◽  
L Veinot-Drebot ◽  
V Ling
Keyword(s):  
Multidrug Resistance ◽  
Gene Duplication ◽  
Gene Family ◽  
Multigene Family ◽  
Rhesus Monkeys ◽  
Genomic Library ◽  
Duplication Event ◽  
Glycoprotein Gene ◽  
P Glycoprotein ◽  
Exon Probe

Overproduction of P-glycoprotein is intimately associated with multidrug resistance. This protein appears to be encoded by a multigene family. Thus, differential expression of different members of this family may contribute to the complexity of the multidrug resistance phenotype. Three lambda genomic clones isolated from a hamster genomic library represent different members of the hamster P-glycoprotein gene family. Using a highly conserved exon probe, we found that the hamster P-glycoprotein gene family consists of three genes. We also found that the P-glycoprotein gene family consists of three genes in mice but has only two genes in humans and rhesus monkeys. The hamster P-glycoprotein genes have similar exon-intron organizations within the 3' region encoding the cytoplasmic domains. We propose that the hamster P-glycoprotein gene family arose from gene duplication. The hamster pgp1 and pgp2 genes appear to be more closely related to each other than either gene is to the pgp3 gene. We speculate that the hamster pgp1 and pgp2 genes arose from a recent gene duplication event and that primates did not undergo this duplication and therefore contain only two P-glycoprotein genes.

Citrus auraptene induces drug efflux transporter P-glycoprotein expression in human intestinal cells

2020 ◽  
Vol 11 (6) ◽  
pp. 5017-5023 ◽  
Author(s):  
Tomohiro Nabekura ◽  
Tatsuya Kawasaki ◽  
Yu Kato ◽  
Kazuyoshi Kawai ◽  
Serena Fiorito ◽  
...  
Keyword(s):  
Protein Expression ◽  
Drug Interactions ◽  
Intestinal Cells ◽  
Drug Efflux ◽  
Efflux Transporter ◽  
Glycoprotein Gene ◽  
P Glycoprotein ◽  
Human Intestinal Cells

Citrus phytochemical auraptene activates the drug efflux transporter P-glycoprotein gene (MDR1) promoter in human intestinal LS174T cells. Auraptene increases protein expression of P-glycoprotein. Auraptene can cause food–drug interactions.

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