A RT-PCR assay for the differential diagnosis of vesicular viral diseases of swine

1998 ◽  
Vol 72 (2) ◽  
pp. 227-235 ◽  
Author(s):  
José Ignacio Núñez ◽  
Esther Blanco ◽  
Teresa Hernández ◽  
Concepción Gómez-Tejedor ◽  
Marı́a Jesus Martı́n ◽  
...  
Keyword(s):  
Differential Diagnosis ◽  
Viral Diseases ◽  
Rt Pcr ◽  
Pcr Assay

Development of rapid, sensitive one-tube duplex RT-PCR assay for specific and differential diagnosis of Chikungunya and dengue

2013 ◽  
Vol 193 (2) ◽  
pp. 521-524 ◽  
Author(s):  
Kallol Saha ◽  
Rushna Firdaus ◽  
Sekhar Chakrabarti ◽  
Provash Chandra Sadhukhan
Keyword(s):  
Differential Diagnosis ◽  
Rt Pcr ◽  
Pcr Assay

A multiplex RT-PCR assay for the rapid and differential diagnosis of classical swine fever and other pestivirus infections

2009 ◽  
Vol 139 (3-4) ◽  
pp. 245-252 ◽  
Author(s):  
Heidy Díaz de Arce ◽  
Lester J. Pérez ◽  
Maria T. Frías ◽  
Rosa Rosell ◽  
Joan Tarradas ◽  
...  
Keyword(s):  
Differential Diagnosis ◽  
Classical Swine Fever ◽  
Rt Pcr ◽  
Pcr Assay

Improved duplex RT-PCR assay for differential diagnosis of mixed infection of duck hepatitis A virus type 1 and type 3 in ducklings

2013 ◽  
Vol 192 (1-2) ◽  
pp. 12-17 ◽  
Author(s):  
Lin-Lin Chen ◽  
Qian Xu ◽  
Rui-Hua Zhang ◽  
Lei Yang ◽  
Jing-Xin Li ◽  
...  
Keyword(s):  
Differential Diagnosis ◽  
Virus Type ◽  
Mixed Infection ◽  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Rt Pcr ◽  
Pcr Assay ◽  
Duck Hepatitis ◽  
Type 3

scholarly journals A highly sensitive and specific gel-based multiplex RT-PCR assay for the simultaneous and differential diagnosis of African swine fever and Classical swine fever in clinical samples

2004 ◽  
Vol 35 (5) ◽  
pp. 551-563 ◽  
Author(s):  
Montserrat Ag�ero ◽  
Jovita Fern�ndez ◽  
Luis Jos� Romero ◽  
Ma Jes�s Zamora ◽  
Carmen S�nchez ◽  
...  
Keyword(s):  
Differential Diagnosis ◽  
African Swine Fever ◽  
Classical Swine Fever ◽  
Clinical Samples ◽  
Rt Pcr ◽  
Pcr Assay ◽  
Highly Sensitive

Rapid and differential diagnosis of foot-and-mouth disease, swine vesicular disease, and vesicular stomatitis by a new multiplex RT-PCR assay

2008 ◽  
Vol 147 (2) ◽  
pp. 301-311 ◽  
Author(s):  
Jovita Fernández ◽  
Montserrat Agüero ◽  
Luis Romero ◽  
Carmen Sánchez ◽  
Sándor Belák ◽  
...  
Keyword(s):  
Differential Diagnosis ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Rt Pcr ◽  
Pcr Assay ◽  
Foot And Mouth ◽  
Vesicular Stomatitis ◽  
Vesicular Disease

1089: Prognostic Value of Molecular Staging of Bladder Cancer with RT -PCR Assay for KRT20: Results at 5 Year-Follow-up

2007 ◽  
Vol 177 (4S) ◽  
pp. 360-360
Author(s):  
Ana Agud ◽  
Maria J. Ribal ◽  
Lourdes Mengual ◽  
Mercedes Marin-Aguilera ◽  
Laura Izquierdo ◽  
...  
Keyword(s):  
Bladder Cancer ◽  
Prognostic Value ◽  
Rt Pcr ◽  
Pcr Assay ◽  
Molecular Staging

Polymerase chain reaction amplification and typing of rotavirus in environmental samples

1995 ◽  
Vol 31 (5-6) ◽  
pp. 371-374 ◽  
Author(s):  
R. Gajardo ◽  
R. M. Pintó ◽  
A. Bosch
Keyword(s):  
Polymerase Chain Reaction ◽  
Environmental Samples ◽  
Polymerase Chain Reaction Amplification ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Pcr Assay ◽  
Group A ◽  
Reaction Amplification ◽  
Stool Specimens ◽  
Polymerase Chain

A reverse transcription polymerase chain reaction (RT-PCR) assay is described that has been developed for the detection and serotyping of group A rotavirus in stool specimens and concentrated and non-concentrated sewage specimens.

Scoring System for the Diagnosis of COVID-19

2020 ◽  
Vol 13 (1) ◽  
pp. 413-414 ◽  
Author(s):  
Mohamed Farouk Allam
Keyword(s):  
Scoring System ◽  
Clinical Symptoms ◽  
False Negative ◽  
Nasopharyngeal Swab ◽  
Rt Pcr ◽  
Pcr Assay ◽  
Negative Results ◽  
False Negative Results ◽  
Symptoms And Signs

Due to the international spread of COVID-19, the difficulty of collecting nasopharyngeal swab specimen from all suspected patients, the costs of RT-PCR and CT, and the false negative results of RT-PCR assay in 41% of COVID-19 patients, a scoring system is needed to classify the suspected patients in order to determine the need for follow-up, home isolation, quarantine or the conduction of further investigations. A scoring system is proposed as a diagnostic tool for suspected patients. It includes Epidemiological Evidence of Exposure, Clinical Symptoms and Signs, and Investigations (if available). This scoring system is simple, could be calculated in a few minutes, and incorporates the main possible data/findings of any patient.

Effective optimization of SARS-CoV-2 laboratory testing variables in an era of supply chain constraints

2020 ◽  
Vol 15 (15) ◽  
pp. 1483-1487
Author(s):  
Nikhil S Sahajpal ◽  
Ashis K Mondal ◽  
Allan Njau ◽  
Sudha Ananth ◽  
Kimya Jones ◽  
...  
Keyword(s):  
Supply Chain ◽  
Rna Extraction ◽  
Nasopharyngeal Swab ◽  
Sample Collection ◽  
Rt Pcr ◽  
Pcr Assay ◽  
Short Supply ◽  
Viral Transport ◽  
3D Printed ◽  
Bridging Studies

RT-PCR-based assays for the detection of SARS-CoV-2 have played an essential role in the current COVID-19 pandemic. However, the sample collection and test reagents are in short supply, primarily due to supply chain issues. Thus, to eliminate testing constraints, we have optimized three key process variables: RNA extraction and RT-PCR reactions, different sample types and media to facilitate SARS-CoV-2 testing. By performing various validation and bridging studies, we have shown that various sample types such as nasopharyngeal swab, bronchioalveolar lavage and saliva, collected using conventional nasopharyngeal swabs, ESwab or 3D-printed swabs and, preserved in viral transport media, universal transport media, 0.9% sodium chloride or Amies media are compatible with RT-PCR assay for COVID-19. Besides, the reduction of PCR reagents by up to fourfold also produces reliable results.

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