Effects of restricted feeding on the activity of hypothalamic Orexin (OX)-A containing neurons and OX2 receptor mRNA level in the paraventricular nucleus of rats

Fujii, Nobuharu, Takeshi Shibata, Sachiko Homma, Haruo Ikegami, Kazuo Murakami, and Hitoshi Miyazaki. Exercise-induced changes in β-adrenergic-receptor mRNA level measured by competitive RT-PCR. J. Appl. Physiol. 82(6): 1926–1931, 1997.—Competitive reverse transcription-polymerase chain reaction (RT-PCR) analysis was used to clarify whether dynamic exercise-induced increases in β-adrenergic-receptor (β-AR) number in human lymphocytes are accompanied by increases in the β-AR mRNA level. Sixteen healthy subjects performed cycle ergometry until exhaustion. Before and immediately after exercise, peripheral blood was drawn from a forearm vein for preparation of lymphocytes. Both the β-AR mRNA level and the β-AR number were significantly increased by exercise. The changes in β-AR mRNA level and β-AR number were significantly correlated ( r = 0.63, P < 0.01). This finding suggests that a rapid increase in β-AR mRNA level might be an early adaptive response of the sympathetic nervous system to dynamic exercise. In vitro incubation of lymphocytes with epinephrine had no effect on β-AR mRNA levels, nor did adenosine 3′,5′-cyclic monophosphate, protein kinase C, or intracellular Ca2+ increase the β-AR mRNA level in vitro. Therefore, it appears that other mechanisms underlie the exercise-induced elevation of β-AR mRNA levels in human lymphocytes.

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The increase in angiotensin type-2 receptor mRNA level by glutamate stimulation in cultured rat cortical cells

The Japanese Journal of Pharmacology ◽

10.1016/s0021-5198(19)45603-5 ◽

1997 ◽

Vol 73 ◽

pp. 275

Author(s):

Ikuko Makino ◽

Kazuhiko Shibata ◽

Hirotomo Shibaguchi ◽

Masami Niwa ◽

Tatsuo Furukawa

Keyword(s):

Mrna Level ◽

Cortical Cells ◽

Receptor Mrna ◽

Angiotensin Type 2 Receptor ◽

Receptor Mrna Level ◽

Angiotensin Type

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Time course of changes in μ-opioid receptor mRNA level by microinjection of antisense oligodeoxynucleotides directed against μ-opioid receptor mRNA into the periaqueductal gray

The Japanese Journal of Pharmacology ◽

10.1016/s0021-5198(19)45129-9 ◽

1997 ◽

Vol 73 ◽

pp. 157

Author(s):

Masanobu Yoshikawa ◽

Kouichi Yokogami ◽

Ken Kitamura ◽

Tomomi Kobayashi ◽

Masayuki Kanal ◽

...

Keyword(s):

Opioid Receptor ◽

Time Course ◽

Mrna Level ◽

Periaqueductal Gray ◽

Antisense Oligodeoxynucleotides ◽

Receptor Mrna ◽

Μ Opioid Receptor ◽

Receptor Mrna Level

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Transient upregulation of the AT2 receptor mRNA level after global ischemia in the rat brain

Neuropeptides ◽

10.1016/s0143-4179(96)90043-8 ◽

1996 ◽

Vol 30 (6) ◽

pp. 596-601 ◽

Cited By ~ 71

Author(s):

I Makino ◽

K Shibata ◽

Y Ohgami ◽

M Fujiwara ◽

T Furukawa

Keyword(s):

Rat Brain ◽

Mrna Level ◽

Global Ischemia ◽

At2 Receptor ◽

Receptor Mrna ◽

Receptor Mrna Level

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Abstract WP266: Deficiency of Angiotensin Converting Enzyme 2 Causes Deterioration of Cognitive Function

Stroke ◽

10.1161/str.47.suppl_1.wp266 ◽

2016 ◽

Vol 47 (suppl_1) ◽

Author(s):

Jun Iwanami ◽

Masaki Mogi ◽

Xiao-Li Wang ◽

Kana Tsukuda ◽

Akinori Higaki ◽

...

Keyword(s):

Cognitive Function ◽

Mrna Level ◽

Ang Ii ◽

Maze Task ◽

Receptor Mrna ◽

Impaired Cognitive Function ◽

Mas Receptor ◽

Morris Water Maze Task ◽

Significant Difference ◽

Receptor Mrna Level

Introduction: The classical renin-angiotensin system (RAS), known as the angiotensin (Ang) converting enzyme (ACE)/Ang II/Ang II type 1 (AT1) receptor axis, induces various organ damage including cognitive decline. On the other hand, the ACE2/Ang-(1-7)/Mas receptor axis has been highlighted as exerting antagonistic actions against the classical RAS axis in the cardiovascular system. However, the roles of the ACE2/Ang-(1-7)/Mas axis in cognitive function largely remain to be elucidated, and we therefore examined possible roles of ACE2 in cognitive function. Methods: Male 10-week-old C57BL6 (wild-type: WT) mice and ACE2 knockout (KO) mice were subjected to the Morris water maze task to evaluate spatial cognitive function. Vascular dementia model were induced by bilateral common carotid artery stenosis (BCAS). The Morris water maze task was performed 6 weeks after BCAS operation. Results: ACE2KO mice exhibited significant impairment of spatial cognitive function, compared with that in WT mice, without significant difference in cerebral blood flow determined by laser speckle flowmetry and morphological changes in the hippocampus between both strains. Superoxide anion production in the hippocampus tended to be increased in ACE2KO mice, with increased mRNA levels of NADPH oxidase subunit in the hippocampus of ACE2KO compared with WT mice. Protein level of superoxide dismutase (SOD) 3 tended to be decreased in ACE2KO mice compared with WT mice. AT1 receptor mRNA level in the hippocampus was higher in ACE2KO mice compared with WT mice. In contrast, AT2 receptor mRNA level in the hippocampus did not differ between the two strains. Mas receptor mRNA was highly expressed in the hippocampus compared with the cortex, with no significant difference between ACE2KO and WT mice. Brain-derived neurotrophic factor (BDNF) mRNA was lower in the hippocampus in ACE2KO mice compared with WT mice. Escape latency after BCAS was prolonged in WT mice compared with sham operated mice, whereas impaired cognitive function in ACE2 KO mice was not further exaggerated after BCAS. Conclusion: Taken together ACE2 deficiency resulted in impaired cognitive function probably due to enhanced oxidative stress and a decrease in BDNF.

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