Assessing viability and cell-associated product of recombinant protein producing Pichia pastoris with flow cytometry

2003 ◽  
Vol 102 (3) ◽  
pp. 281-290 ◽  
Author(s):  
Hubertus Hohenblum ◽  
Nicole Borth ◽  
Diethard Mattanovich
Keyword(s):  
Flow Cytometry ◽  
Pichia Pastoris ◽  
Recombinant Protein

scholarly journals Macroscopic modeling of bioreactors for recombinant protein producing Pichia pastoris in defined medium

2020 ◽  
Author(s):  
Moo Sun Hong ◽  
M. Lourdes Velez‐Suberbie ◽  
Andrew J. Maloney ◽  
Andrew Biedermann ◽  
Kerry R. Love ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Recombinant Protein ◽  
Defined Medium ◽  
Macroscopic Modeling

scholarly journals Generation of diploid Pichia pastoris strains by mating and their application for recombinant protein production

2012 ◽  
Vol 11 (1) ◽  
pp. 91 ◽  
Author(s):  
Ming-Tang Chen ◽  
Song Lin ◽  
Ishaan Shandil ◽  
Dewan Andrews ◽  
Terrance A Stadheim ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Recombinant Protein ◽  
Recombinant Protein Production ◽  
Protein Production

A prime-boost immunization with Tc52 N-terminal domain DNA and the recombinant protein expressed in Pichia pastoris protects against Trypanosoma cruzi infection

Vaccine ◽  
2016 ◽  
Vol 34 (28) ◽  
pp. 3243-3251 ◽  
Author(s):  
Marina N. Matos ◽  
Andrés Sánchez Alberti ◽  
Celina Morales ◽  
Silvia I. Cazorla ◽  
Emilio L. Malchiodi
Keyword(s):  
Pichia Pastoris ◽  
Recombinant Protein ◽  
Trypanosoma Cruzi ◽  
Terminal Domain ◽  
Boost Immunization ◽  
Cruzi Infection

Quo vadis? The challenges of recombinant protein folding and secretion in Pichia pastoris

2015 ◽  
Vol 99 (7) ◽  
pp. 2925-2938 ◽  
Author(s):  
Verena Puxbaum ◽  
Diethard Mattanovich ◽  
Brigitte Gasser
Keyword(s):  
Protein Folding ◽  
Pichia Pastoris ◽  
Recombinant Protein ◽  
Quo Vadis

Expression of the Necator americanus hookworm larval antigen Na-ASP-2 in Pichia pastoris and purification of the recombinant protein for use in human clinical trials

Vaccine ◽  
2005 ◽  
Vol 23 (39) ◽  
pp. 4754-4764 ◽  
Author(s):  
Gaddam Narsa Goud ◽  
Maria Elena Bottazzi ◽  
Bin Zhan ◽  
Susana Mendez ◽  
Vehid Deumic ◽  
...  
Keyword(s):  
Clinical Trials ◽  
Pichia Pastoris ◽  
Recombinant Protein ◽  
Necator Americanus

scholarly journals The effect of α-mating factor secretion signal mutations on recombinant protein expression in Pichia pastoris

Gene ◽  
2013 ◽  
Vol 519 (2) ◽  
pp. 311-317 ◽  
Author(s):  
Geoff P. Lin-Cereghino ◽  
Carolyn M. Stark ◽  
Daniel Kim ◽  
Jennifer Chang ◽  
Nadia Shaheen ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Recombinant Protein ◽  
Protein Expression ◽  
Recombinant Protein Expression ◽  
Secretion Signal ◽  
Mating Factor ◽  
Factor Secretion
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