Erratum to “Effect of DNA/liposome mixing ratio on the physicochemical characteristics, cellular uptake and intracellular trafficking of plasmid DNA/cationic liposome complexes and subsequent gene expression”

2000 ◽  
Vol 69 (1) ◽  
pp. 219-220 ◽  
Author(s):  
Fuminori Sakurai ◽  
Rui Inoue ◽  
Yasunobu Nishino ◽  
Ayumu Okuda ◽  
Osamu Matsumoto ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cellular Uptake ◽  
Plasmid Dna ◽  
Intracellular Trafficking ◽  
Cationic Liposome ◽  
Physicochemical Characteristics ◽  
Mixing Ratio

scholarly journals Gene Transfer into the Lung by Nanoparticle Dextran-Spermine/Plasmid DNA Complexes

2010 ◽  
Vol 2010 ◽  
pp. 1-10 ◽  
Author(s):  
Syahril Abdullah ◽  
Wai Yeng Wendy-Yeo ◽  
Hossein Hosseinkhani ◽  
Mohsen Hosseinkhani ◽  
Ehab Masrawa ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Transfer ◽  
Cell Lines ◽  
Plasmid Dna ◽  
Mixing Ratio ◽  
Systemic Delivery ◽  
Clear Trend ◽  
Assay Time

A novel cationic polymer, dextran-spermine (D-SPM), has been found to mediate gene expression in a wide variety of cell lines andin vivothrough systemic delivery. Here, we extended the observations by determining the optimal conditions for gene expression of D-SPM/plasmid DNA (D-SPM/pDNA) in cell lines and in the lungs of BALB/c mice via instillation delivery.In vitrostudies showed that D-SPM could partially protect pDNA from degradation by nuclease and exhibited optimal gene transfer efficiency at D-SPM to pDNA weight-mixing ratio of 12. In the lungs of mice, the levels of gene expression generated by D-SPM/pDNA are highly dependent on the weight-mixing ratio of D-SPM to pDNA, amount of pDNA in the complex, and the assay time postdelivery. Readministration of the complex at day 1 following the first dosing showed no significant effect on the retention and duration of gene expression. The study also showed that there was a clear trend of increasing size of the complexes as the amount of pDNA was increased, where the sizes of the D-SPM/pDNA complexes were within the nanometer range.

Gene expression in an intact ex-vivo skin tissue model following percutaneous delivery of cationic liposome–plasmid DNA complexes

2000 ◽  
Vol 197 (1-2) ◽  
pp. 233-238 ◽  
Author(s):  
James C Birchall ◽  
Claire Marichal ◽  
Lee Campbell ◽  
Ashraf Alwan ◽  
Jonathan Hadgraft ◽  
...  
Keyword(s):  
Gene Expression ◽  
Plasmid Dna ◽  
Ex Vivo ◽  
Cationic Liposome ◽  
Skin Tissue ◽  
Dna Complexes ◽  
Tissue Model ◽  
Model Following

Linear forms of plasmid DNA are superior to supercoiled structures as active templates for gene expression in plant protoplasts

1988 ◽  
Vol 11 (4) ◽  
pp. 517-527 ◽  
Author(s):  
Nurit Ballas ◽  
Nehama Zakai ◽  
Devorah Friedberg ◽  
Abraham Loyter
Keyword(s):  
Gene Expression ◽  
Plasmid Dna ◽  
Plant Protoplasts ◽  
Linear Forms
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