735 TLR MEDIATED CYTOKINE PRODUCTION IN PRIMARY HUMAN HEPATOCYTES AND NON-PARENCHYMAL LIVER CELLS

2010 ◽  
Vol 52 ◽  
pp. S286
Author(s):  
K. Visvanathan ◽  
N. Skinner ◽  
A. Thompson ◽  
S. Locarnini ◽  
A. Testro ◽  
...  
Keyword(s):  
Cytokine Production ◽  
Liver Cells ◽  
Human Hepatocytes ◽  
Primary Human Hepatocytes ◽  
Parenchymal Liver

scholarly journals Protocol for Isolation of Primary Human Hepatocytes and Corresponding Major Populations of Non-parenchymal Liver Cells

10.3791/53069 ◽  
2016 ◽  
Author(s):  
Victoria Kegel ◽  
Daniela Deharde ◽  
Elisa Pfeiffer ◽  
Katrin Zeilinger ◽  
Daniel Seehofer ◽  
...  
Keyword(s):  
Liver Cells ◽  
Human Hepatocytes ◽  
Primary Human Hepatocytes ◽  
Parenchymal Liver

scholarly journals Metabolism of the Anti-Hepatitis C Virus Nucleoside β-d-N4-Hydroxycytidine in Different Liver Cells

2004 ◽  
Vol 48 (12) ◽  
pp. 4636-4642 ◽  
Author(s):  
Brenda I. Hernandez-Santiago ◽  
Thierry Beltran ◽  
Lieven Stuyver ◽  
Chung K. Chu ◽  
Raymond F. Schinazi
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Whole Blood ◽  
Hepg2 Cells ◽  
Liver Cells ◽  
Human Hepatocytes ◽  
Catabolic Pathway ◽  
Human Whole Blood ◽  
Primary Human Hepatocytes ◽  
Intracellular Metabolism

ABSTRACT β-d-N 4-Hydroxycytidine (NHC) was found to have selective anti-hepatitis C virus (HCV) activity in the HCV replicon system (clone A). The intracellular metabolism of tritiated NHC was investigated in the HCV replicon system, Huh-7 cells, HepG2 cells, and primary human hepatocytes. Incubation of cells with 10 μM radiolabeled NHC demonstrated extensive and rapid phosphorylation in all liver cells. Besides the 5′-mono, -di-, and -triphosphate metabolites of NHC, other metabolites were characterized. These included cytidine and uridine mono-, di-, and triphosphates. UTP was the predominant early metabolite in Huh-7 cells and primary human hepatocytes, suggesting deamination of NHC as the primary catabolic pathway. The intracellular half-lives of radiolabeled NHC-triphosphate and of CTP and UTP derived from NHC incubation in Huh-7 cells were calculated to be 3.0 ± 1.3, 10.4 ± 3.3, and 13.2 ± 3.5 h (means ± standard deviations), respectively. Studies using monkey and human whole blood demonstrated more-rapid deamination and oxidation in monkey cells than in human cells, suggesting that NHC may not persist long enough in plasma to be delivered to liver cells.

scholarly journals Featured Article: Isolation, characterization, and cultivation of human hepatocytes and non-parenchymal liver cells

2014 ◽  
Vol 240 (5) ◽  
pp. 645-656 ◽  
Author(s):  
Elisa Pfeiffer ◽  
Victoria Kegel ◽  
Katrin Zeilinger ◽  
Jan G Hengstler ◽  
Andreas K Nüssler ◽  
...  
Keyword(s):  
Liver Cells ◽  
Human Hepatocytes ◽  
Parenchymal Liver

Evaluation of Flutamide Genotoxicity in Rats and in Primary Human Hepatocytes

2000 ◽  
Vol 86 (3) ◽  
pp. 129-134 ◽  
Author(s):  
Antonietta MartelliNote ◽  
Giulia Brambilla Campart ◽  
Roberto Carrozzino ◽  
Marco Ghia ◽  
Francesca Mattioli ◽  
...  
Keyword(s):  
Human Hepatocytes ◽  
Primary Human Hepatocytes

Continuous lipid treatment of primary human hepatocytes leads to an elevation of oxidative stress

2013 ◽  
Vol 51 (01) ◽  
Author(s):  
T Schulz ◽  
V Kegel ◽  
B Burkhardt ◽  
D Seehofer ◽  
M Glanemann ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Human Hepatocytes ◽  
Primary Human Hepatocytes

Uptake, Internalization and Degradation of the Novel Plasminogen Activator Reteplase (BM 06.022) in the Rat

1995 ◽  
Vol 74 (06) ◽  
pp. 1501-1510 ◽  
Author(s):  
J Kuiper ◽  
H van de Bilt ◽  
U Martin ◽  
Th J C van Berkel
Keyword(s):  
Plasminogen Activator ◽  
Liver Cells ◽  
The Novel ◽  
Uptake Mechanism ◽  
Liver Uptake ◽  
Lysosomal Compartment ◽  
Β Phase ◽  
Α Phase ◽  
Parenchymal Liver

SummaryThe catabolism of the novel plasminogen activator reteplase (BM 06.022) was described. For this purpose BM 06.022 was radiolabelled with l25I or with the accumulating label l25I-tyramine cellobiose (l25I-TC).BM 06.022 was injected at a pharmacological dose of 380 μg/kg b.w. and it was cleared from the plasma in a biphasic manner with a half-life of about 1 min in the α-phase and t1/2of 20-28 min in the β-phase. 28% and 72% of the injected dose was cleared in the α-phase and β-phase, respectively. Initially liver, kidneys, skin, bones, lungs, spleen, and muscles contributed mainly to the plasma clearance. Only liver and the kidneys, however, were responsible for the uptake and subsequent degradation of BM 06.022 and contributed for 75% to the catabolism of BM 06.022. BM 06.022 was degraded in the lysosomal compartment of both organs. Parenchymal liver cells were responsible for 70% of the liver uptake of BM 06.022. BM 06.022 associated rapidly to isolated rat parenchymal liver cells and was subsequently degraded in the lysosomal compartment of these cells. BM 06.022 bound with low-affinity to the parenchymal liver cells (550 nM) and the binding of BM 06.022 could be displaced by t-PA (IC50 5.6 nM), indicating that the low-density lipoprotein receptor-related protein (LRP) could be involved in the binding of BM 06.022. GST-RAP, which is an inhibitor of LRP, could in vivo significantly inhibit the uptake of BM 06.022 in the liver.It is concluded that BM 06.022 is metabolized primarily in the liver and the kidneys. These organs take up and degrade BM 06.022 in the lysosomes. The uptake mechanism of BM 06.022 in the kidneys is unknown, while LRP is responsible for a low-affinity binding and uptake of BM 06.022 in parenchymal liver cells.

Characterisation of primary human hepatocytes after transplantation into the upa mouse model

2006 ◽  
Vol 44 (01) ◽  
Author(s):  
M Lütgehetmann ◽  
T Volz ◽  
J Pollok ◽  
L Fischer ◽  
A Quaas ◽  
...  
Keyword(s):  
Mouse Model ◽  
Human Hepatocytes ◽  
Primary Human Hepatocytes
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