563 A NOVEL RECOMBINANT FORM OF THE HUMAN MANGANESE SUPEROXIDE DISMUTASE PROTECTS RAT LIVER GRAFTS PROCURED FOR TRANSPLANTATION

Background. We report a case of bilateral posterior subcapsular cataracts (PSCs) in a 24-year-old man with an allergic conjunctivitis history caused by a long-term therapy with glucocorticoids.Case Presentation. The patient showed a visual acuity of 9/10 for both eyes. He followed a therapy with ketotifen and bilastine for four years. During the last six months before our evaluation, he was treated with chloramphenicol and betamethasone, interrupted for onset of cataracts and increased intraocular pressure. We treated him with ophthalmic gel preparation containing a new recombinant form of manganese superoxide dismutase (rMnSOD) at a concentration of 12.5 μg/mL, only for the right eye, while left eye was treated with standard protocol of Bendazac-lysine g 0.5.Conclusion. This case report shows the protective effects of rMnSOD versus PSC disease, probably due to the capacity of rMnSOD of countering free radical species.

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Diethyldithiocarbamate Treatment Up Regulates Manganese Superoxide Dismutase Gene Expression in Rat Liver

Biochemical and Biophysical Research Communications ◽

10.1006/bbrc.1996.0441 ◽

1996 ◽

Vol 220 (3) ◽

pp. 546-552 ◽

Cited By ~ 6

Author(s):

Silvia Borrello ◽

Maria Emilia De Leo ◽

Matteo Landriscina ◽

Bernardetta Palazzotti ◽

Tommaso Galeotti

Keyword(s):

Gene Expression ◽

Superoxide Dismutase ◽

Rat Liver ◽

Manganese Superoxide Dismutase ◽

Manganese Superoxide ◽

Superoxide Dismutase Gene

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Redox up-regulated expression of rat liver manganese superoxide dismutase and Bcl-2 by thyroid hormone is associated with inhibitor of κB-α phosphorylation and nuclear factor-κB activation

Journal of Endocrinology ◽

10.1677/joe.1.06261 ◽

2005 ◽

Vol 186 (3) ◽

pp. 539-547 ◽

Cited By ~ 36

Author(s):

Virginia Fernández ◽

Gladys Tapia ◽

Patricia Varela ◽

Iván Castillo ◽

Catalina Mora ◽

...

Keyword(s):

Superoxide Dismutase ◽

Dna Binding ◽

Rat Liver ◽

Manganese Superoxide Dismutase ◽

Nuclear Factor Κb ◽

Protective Mechanism ◽

Time Interval ◽

Nuclear Factor ◽

Manganese Superoxide ◽

Tnf Α

Recently, we demonstrated that 3,3′,5-triiodothyronine (T3) induces oxidative stress in rat liver, with enhancement in the DNA binding of nuclear factor-κB (NF-κB) and the NF-κB-dependent expression of tumor necrosis factor-α (TNF-α). In this study, we show that T3 administration (daily doses of 0.1 mg/kg i.p. for three consecutive days) elicited a calorigenic response and higher liver O2 consumption rates, with increased serum levels of TNF-α (ELISA), liver inhibitor of κB (IκB-α) phosphorylation (Western blot analysis), and hepatic NF-κB DNA binding (EMSA) at 56–72 h after treatment. Within this time interval, liver manganese superoxide dismutase (MnSOD) activity and the protein expression of MnSOD and Bcl-2 are enhanced. These changes are abrogated by the administration of α-tocopherol (100 mg/kg i.p.) prior to T3. It is concluded that T3 treatment leads to the redox upregulation of MnSOD and Bcl-2 in rat liver, in association with TNF-α release and activation of the IκB-α kinase/NF-κB cascade, which may constitute a protective mechanism against free radical toxicity involving cell death signaling.

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