Transgenic sweet potato plants carrying the delta-endotoxin gene from Bacillus thuringiensis var. tenebrionis.

Plant Science ◽  
1998 ◽  
Vol 139 (2) ◽  
pp. 175-184 ◽  
Author(s):  
R. Morán ◽  
R. Garcı́a ◽  
A. López ◽  
Z. Zaldúa ◽  
J. Mena ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
Sweet Potato ◽  
Potato Plants ◽  
Delta Endotoxin

Accumulation of carbon and nitrogen compounds in sweet potato plants grown under deficiency of N, P, or K nutrients

1995 ◽  
Vol 41 (3) ◽  
pp. 557-566 ◽  
Author(s):  
Mitsuru Osaki ◽  
Hiroyuki Ueda ◽  
Takuro Shinano ◽  
Hirokazu Matsui ◽  
Toshiaki Tadano
Keyword(s):  
Sweet Potato ◽  
Nitrogen Compounds ◽  
Carbon And Nitrogen ◽  
Potato Plants

scholarly journals Analysis of the molecular basis of insecticidal specificity of Bacillus thuringiensis crystal δ-endotoxin

1987 ◽  
Vol 248 (1) ◽  
pp. 197-201 ◽  
Author(s):  
M Z Haider ◽  
D J Ellar
Keyword(s):  
Bacillus Thuringiensis ◽  
Membrane Proteins ◽  
Insect Cell ◽  
Receptor Interaction ◽  
Dual Specificity ◽  
Delta Endotoxin ◽  
Cell Membrane Proteins ◽  
Toxin Receptor ◽  
Osmotic Lysis ◽  
Initial Interaction

The mechanism of action and receptor binding of a dual-specificity Bacillus thuringiensis var. aizawai ICl delta-endotoxin was studied using insect cell culture. The native protoxin was labelled with 125I, proteolytically activated and the affinity of the resulting preparations for insect cell-membrane proteins was studied by blotting. The active preparations obtained by various treatments had characteristic specificity associated with unique polypeptides, and showed affinity for different membrane proteins. The lepidopteran-specific preparation (trypsin-treated protoxin containing 58 and 55 kDa polypeptides) bound to two membrane proteins in the lepidopteran cells but none in the dipteran cells. The dipteran-specific preparation (protoxin treated sequentially with trypsin and Aedes aegypti gut proteases, containing a 53 kDa polypeptide) bound to a 90 kDa membrane protein in the dipteran (A. aegypti) cells but bound to none in the lepidopteran cells or Drosophila melanogaster cells. The toxicity of trypsin-activated delta-endotoxin was completely inhibited by preincubation with D-glucose, suggesting a role for this carbohydrate in toxin-receptor interaction. The toxicity was also decreased by osmotic protectants to an extent proportional to their viscometric radius. These results support a proposal that initial interaction of toxin with a unique receptor determines the specificity of the toxin, following which cell death occurs by a mechanism of colloid osmotic lysis.

scholarly journals Plant age and genotype affect the bacterial community composition in the tuber rhizosphere of field-grown sweet potato plants

2014 ◽  
Vol 88 (2) ◽  
pp. 424-435 ◽  
Author(s):  
Joana M. Marques ◽  
Thais F. da Silva ◽  
Renata E. Vollu ◽  
Arie F. Blank ◽  
Guo-Chun Ding ◽  
...  
Keyword(s):  
Bacterial Community ◽  
Sweet Potato ◽  
Community Composition ◽  
Bacterial Community Composition ◽  
Plant Age ◽  
Potato Plants

scholarly journals Effects of Bacillus thuringiensis δ-Endotoxin on Insect and Mammalian Cells In Vitro

1980 ◽  
Vol 15 (2) ◽  
pp. 133-139 ◽  
Author(s):  
Junko NISHITSUTSUJI-UWO ◽  
Yasuhisa ENDO ◽  
Michio HIMENO
Keyword(s):  
Bacillus Thuringiensis ◽  
Mammalian Cells ◽  
Delta Endotoxin

scholarly journals Se-O Bond Is Unique to High Se Enriched Sweet Potato Stem Protein with Better Antioxidant Ability

Foods ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 3064
Author(s):  
Qi Gao ◽  
Jia-Le Wu ◽  
Lan-Ping Jiang ◽  
Su-Qi Sun ◽  
Xue-Jun Gu ◽  
...  
Keyword(s):  
Sweet Potato ◽  
Energy Dispersive Spectrometry ◽  
Structural Characteristics ◽  
Radical Scavenging ◽  
Dpph Radical ◽  
Enrichment Method ◽  
Potato Plants ◽  
Ft Ir ◽  
Dpph Radical Scavenging ◽  
Thermal Stability Of

Sweet potato plants were treated with selenium (Se). Spraying Se on the sweet potato leaves was an effective Se enrichment method and proteins were extracted from the sweet potato stem. The structural characteristics of the protein were investigated. Fourier transform infrared spectroscopy (FT-IR) detected more signals from the Se-enriched sweet potato stem protein (SSP), and the number of forms of Se chemical bonds gradually increased with increasing Se content, such as the Se-O bond in high Se-enriched SSP, indicating altered secondary structures.Scanning electron microscopy-energy dispersive spectrometry (SEM-EDS) indicated more Se atoms in the Se-enriched SSPs (SSSPs). The DSC results revealed that Se enrichment enhanced the thermal stability of the samples. Moreover, selenomethionine (SeMet), selenocystine (SeCys2), and methylselenocysteine (MeSeCys) were determined to be the main Se forms in the SSSPs. Furthermore, the SSSPs showed relatively higher superoxide anion radical and DPPH radical scavenging activities than the blank, which indicates that SSSPs can be used as antioxidants. By recovering the proteins, the agricultural by-product—sweet potato stem can be further utilized, and the obtained Se-enriched proteins may contribute to human health.

scholarly journals Herbicide Evaluation for Sweet Potatoes

1969 ◽  
Vol 66 (4) ◽  
pp. 254-260 ◽  
Author(s):  
Lii-Chyuan Liu ◽  
Edwin Acevedo-Borrero ◽  
F. H. Ortiz
Keyword(s):  
Weed Control ◽  
Sweet Potato ◽  
Tuber Yield ◽  
Potato Cultivar ◽  
Weed Competition ◽  
Sweet Potatoes ◽  
Minimum Rate ◽  
Potato Plants ◽  
Herbicide Treatments ◽  
Rate Controlled

Two herbicide experiments were carried out in 1980 to evaluate Alachor and Metribuzin alone or combined for weed control in sweet potato cultivar Miguela at the Isabela and Fortuna Substations, Metribuzin at 1.12 kg ai/ha rate controlled effectively both broadleaf weeds and grasses. A minimum rate of 6.73 kg ai/ha of Alachor was needed for acceptable weed control. Metribuzin at the 1.12 kg al/ha rate in combination with Alachor at the 3.36 kg ai/ha rate provided the best weed control. There was no visible herbicide injury to sweet potato plants at the Isabela Substation. Moderate crop injury as a consequence of Metribuzin application at 2.24 kg ai/ha was apparent at the Fortuna Substation. The highest tuber yield was obtained with Metribuzin at 1.12 kg ai/ha in combination with Alachor at 3.36 kg ai/ha at both Substations. Metribuzin at 1.12 kg ai/ha rate alone or in combination with any other herbicide also produced good tuber yield. Sweet potatoes with standard herbicide treatments, Diphenamid and Chloramben, yielded poorly because of weed competition.

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