The bulb and stem nematode, Ditylenchus dipsaci, attacks narcissus, iris, red clover and strawberry in the Pacific Northwest. The isolations from each of these important crops are herein described as strains.Preliminary studies of the transfer of these strains establish the existence of three strains of D. dipsaci in the Pacific Northwest, viz.:(i) Red clover strain; characterized by causing swollen crowns and stunt in red clover seedlings.(ii) Strawberry strain; characterized by a limited host range, swollen crowns in strawberry seedlings, and entrance into red clover seedlings without visible tissue reactions.(iii) Narcissus and iris strain; characterized by a wide host range and entrance into clover and strawberry seedlings without visible tissue reactions.No satisfactory technique of establishing the host range of the biological strains of D. dipsaci has been developed. The clamping of glass rings filled with a nematode suspension in moist pulverized peat to the foliage of test plants did not affect the test plants in a constant manner. The examination of seedlings after clarification in a lacto-phenol solution containing acid fuchsin gave more constant results. The seedlings were removed from infested soil shortly after they appeared above ground.The reports of host specificity of the red clover strain were not confirmed, for the red clover strain entered white clover and alfalfa, hitherto considered resistant. Likewise, the reports of host specificity of the narcissus strain were not supported by our experimental results. The narcissus strain entered red clover and oats, also considered resistant hitherto.