scholarly journals A Draft Genome Sequence of Pseudomonas syringae pv. tomato T1 Reveals a Type III Effector Repertoire Significantly Divergent from That of Pseudomonas syringae pv. tomato DC3000

2009 ◽  
Vol 22 (1) ◽  
pp. 52-62 ◽  
Author(s):  
Nalvo F. Almeida ◽  
Shuangchun Yan ◽  
Magdalen Lindeberg ◽  
David J. Studholme ◽  
David J. Schneider ◽  
...  
Keyword(s):  
Host Range ◽  
Host Specificity ◽  
Pseudomonas Syringae ◽  
Genome Sequence ◽  
Draft Genome ◽  
Draft Genome Sequence ◽  
Tomato Dc3000 ◽  
Type Iii ◽  
Host Range Determination ◽  
Range Determination

Diverse gene products including phytotoxins, pathogen-associated molecular patterns, and type III secreted effectors influence interactions between Pseudomonas syringae strains and plants, with additional yet uncharacterized factors likely contributing as well. Of particular interest are those interactions governing pathogen-host specificity. Comparative genomics of closely related pathogens with different host specificity represents an excellent approach for identification of genes contributing to host-range determination. A draft genome sequence of Pseudomonas syringae pv. tomato T1, which is pathogenic on tomato but nonpathogenic on Arabidopsis thaliana, was obtained for this purpose and compared with the genome of the closely related A. thaliana and tomato model pathogen P. syringae pv. tomato DC3000. Although the overall genetic content of each of the two genomes appears to be highly similar, the repertoire of effectors was found to diverge significantly. Several P. syringae pv. tomato T1 effectors absent from strain DC3000 were confirmed to be translocated into plants, with the well-studied effector AvrRpt2 representing a likely candidate for host-range determination. However, the presence of avrRpt2 was not found sufficient to explain A. thaliana resistance to P. syringae pv. tomato T1, suggesting that other effectors and possibly type III secretion system–independent factors also play a role in this interaction.

scholarly journals Draft Genome Sequence of the Endophytic Bacterium Variovorax paradoxus KB5, Which Has Antagonistic Activity against a Phytopathogen, Pseudomonas syringae pv. tomato DC3000

2017 ◽  
Vol 5 (36) ◽  
Author(s):  
Chi Eun Hong ◽  
Sung Hee Jo ◽  
Ick-Hyun Jo ◽  
Haeyoung Jeong ◽  
Jeong Mee Park
Keyword(s):  
Pseudomonas Syringae ◽  
Genome Sequence ◽  
Draft Genome ◽  
Endophytic Bacterium ◽  
Biosynthetic Gene Cluster ◽  
Antagonistic Activity ◽  
Draft Genome Sequence ◽  
Tomato Dc3000 ◽  
Content Type ◽  
Variovorax Paradoxus

ABSTRACT Variovorax paradoxus KB5, isolated from the inside of Arabidopsis thaliana leaves, showed antibacterial activity against the phytopathogen Pseudomonas syringae pv. tomato DC3000. Here, we report a draft genome sequence of V. paradoxus KB5, which contains a delftibactin-like nonribosomal peptide biosynthetic gene cluster.

scholarly journals A Pseudomonas syringae pv. tomato DC3000 Hrp (Type III Secretion) Deletion Mutant Expressing the Hrp System of Bean Pathogen P. syringae pv. syringae 61 Retains Normal Host Specificity for Tomato

2003 ◽  
Vol 16 (1) ◽  
pp. 43-52 ◽  
Author(s):  
Derrick E. Fouts ◽  
Jorge L. Badel ◽  
Adela R. Ramos ◽  
Ryan A. Rapp ◽  
Alan Collmer
Keyword(s):  
Host Range ◽  
Resistance Gene ◽  
Host Specificity ◽  
Pseudomonas Syringae ◽  
Type Iii Secretion ◽  
Secretion System ◽  
Effector Proteins ◽  
Tomato Dc3000 ◽  
Tomato Plants ◽  
Type Iii

The plant pathogenic species Pseudomonas syringae is divided into numerous pathovars based on host specificity. For example, P. syringae pv. tomato DC3000 is pathogenic on tomato and Arabidopsis, whereas P. syringae pv. syringae 61 is pathogenic on bean. The ability of P. syringae strains to elicit the hypersensitive response (HR) in non-hosts or be pathogenic (or parasitic) in hosts is dependent on the Hrp (type III secretion) system and effector proteins this system is thought to inject into plant cells. To test the role of the Hrp system in determining host range, the hrp/hrc gene cluster (hrpK through hrpR) was deleted from DC3000 and complemented in trans with the orthologous cluster from strain 61. Mutant CUCPB5114 expressing the bean pathogen Hrp system on plasmid pCPP2071 retained the ability of wild-type DC3000 to elicit the HR in bean, to grow and cause bacterial speck in tomato, and to elicit a cultivar-specific (gene-for-gene) HR in tomato plants carrying the Pto resistance gene. However, the symptoms produced in compatible tomato plants involved markedly reduced chlorosis, and CUCPB5114(pCPP2071) did not grow or produce symptoms in Arabidopsis Col-0 although it was weakly virulent in NahG Arabidopsis. A hypersensitive-like collapse was produced by CUCPB5114(pCPP2071) in Arabidopsis Col-0 at 1 × 107 CFU/ml, but only if the bacteria also expressed AvrB, which is recognized by the RPM1 resistance gene in Col-0 and confers incompatibility. These observations support the concept that the P. syringae effector proteins, rather than secretion system components, are the primary determinants of host range at both the species and cultivar levels of host specificity.

scholarly journals A draft genome sequence and functional screen reveals the repertoire of type III secreted proteins of Pseudomonas syringae pathovar tabaci 11528

BMC Genomics ◽  
2009 ◽  
Vol 10 (1) ◽  
pp. 395 ◽  
Author(s):  
David J Studholme ◽  
Selena Ibanez ◽  
Daniel MacLean ◽  
Jeffery L Dangl ◽  
Jeff H Chang ◽  
...  
Keyword(s):  
Pseudomonas Syringae ◽  
Genome Sequence ◽  
Draft Genome ◽  
Secreted Proteins ◽  
Draft Genome Sequence ◽  
Type Iii ◽  
Functional Screen

scholarly journals A draft genome sequence and functional screen reveals the repertoire of type III secreted proteins of Pseudomonas syringae pathovar tabaci 11528

BMC Genomics ◽  
2009 ◽  
Vol 10 (1) ◽  
pp. 569 ◽  
Author(s):  
David J Studholme ◽  
Selena Ibanez ◽  
Daniel MacLean ◽  
Jeffery L Dangl ◽  
Jeff H Chang ◽  
...  
Keyword(s):  
Pseudomonas Syringae ◽  
Genome Sequence ◽  
Draft Genome ◽  
Secreted Proteins ◽  
Draft Genome Sequence ◽  
Type Iii ◽  
Functional Screen

scholarly journals Draft Genome Sequence of the Enteropathogenic Bacterium Campylobacter jejuni Strain cj255

2015 ◽  
Vol 3 (5) ◽  
Author(s):  
Fariha Masood Siddiqui ◽  
Muhammad Ibrahim ◽  
Nighat Noureen ◽  
Zobia Noreen ◽  
Richard W. Titball ◽  
...  
Keyword(s):  
Global Health ◽  
Host Range ◽  
Campylobacter Jejuni ◽  
Genome Sequence ◽  
Draft Genome ◽  
Epidemiological Studies ◽  
Draft Genome Sequence ◽  
Broad Host Range ◽  
Bacterial Gastroenteritis

The enteropathogen Campylobacter jejuni is a global health disaster, being one of the leading causes of bacterial gastroenteritis. Here, we present the draft genome sequence of C. jejuni strain cj255, isolated from a chicken source in Islamabad, Pakistan. The draft genome sequence will aid in epidemiological studies and quarantine of this broad-host-range pathogen.

scholarly journals Draft Genome Sequence of Pseudomonas syringae pv. persicae NCPPB 2254

2015 ◽  
Vol 3 (3) ◽  
Author(s):  
Wenjun Zhao ◽  
Hongshan Jiang ◽  
Qian Tian ◽  
Jie Hu
Keyword(s):  
Pseudomonas Syringae ◽  
Genome Sequence ◽  
Draft Genome ◽  
Draft Genome Sequence

scholarly journals Draft Genome Sequence of Pseudomonas syringae RAYR-BL, a Strain Isolated from Natural Accessions of Arabidopsis thaliana Plants

2022 ◽  
Author(s):  
Isabel Fuenzalida-Valdivia ◽  
Maria Victoria Gangas ◽  
Diego Zavala ◽  
Ariel Herrera-Vásquez ◽  
Fabrice Roux ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Pseudomonas Syringae ◽  
Genome Sequence ◽  
Plant Pathogen ◽  
Environmental Conditions ◽  
Draft Genome ◽  
Draft Genome Sequence ◽  
Content Type ◽  
Molecular Features ◽  
Plant Pathogen Interaction

Here, we report the genome sequence of the P. syringae strain RAYR-BL, isolated from natural accessions of Arabidopsis plants. The draft genome sequence consists of 5.85 Mbp assembled in 110 contigs. The study of P. syringae RAYR-BL is a valuable tool to investigate molecular features of plant-pathogen interaction under environmental conditions.

scholarly journals First Draft Genome Sequence of a Pearl Millet Blast Pathogen, Magnaporthe grisea Strain PMg_Dl, Obtained Using PacBio Single-Molecule Real-Time and Illumina NextSeq 500 Sequencing

2019 ◽  
Vol 8 (20) ◽  
Author(s):  
Ganesan Prakash ◽  
Aundy Kumar ◽  
Neelam Sheoran ◽  
Rashmi Aggarwal ◽  
Chellapilla Tara Satyavathi ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Host Range ◽  
Pearl Millet ◽  
Genome Sequence ◽  
Single Molecule ◽  
Magnaporthe Grisea ◽  
Draft Genome ◽  
Draft Genome Sequence ◽  
Content Type ◽  
Genome Information

The first draft genome sequence of the pearl millet blast pathogen Magnaporthe grisea PMg_Dl from India is presented. The genome information of M. grisea will be useful to understand the Magnaporthe speciation, genetic diversity, environmental adaptation, and pathogenic and host range determinants.

scholarly journals Draft Genome Sequence of a Diazotrophic, Plant Growth–Promoting Rhizobacterium of thePseudomonas syringaeComplex

2016 ◽  
Vol 4 (5) ◽  
Author(s):  
Cheryl L. Patten ◽  
Haeyoung Jeong ◽  
Andrew J. C. Blakney ◽  
Natalie Wallace
Keyword(s):  
Nitrogen Fixation ◽  
Plant Growth ◽  
Pseudomonas Syringae ◽  
Genome Sequence ◽  
Draft Genome ◽  
Draft Genome Sequence ◽  
Protein Coding ◽  
Protein Coding Genes ◽  
Plant Growth Promoting ◽  
Growth Promoting

We report here the draft genome sequence ofPseudomonas syringaeGR12-2, a nitrogen-fixing, plant growth–promoting bacterium, isolated from the rhizosphere of an Arctic grass. The 6.6-Mbp genome contains 5,676 protein-coding genes, including a nitrogen-fixation island similar to that inP. stutzeri.

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