Unusual manifestations of secondary syphilis and abnormal humoral immune response to Treponema pallidum antigens in a homosexual man with asymptomatic human immunodeficiency virus infection

The gp120 envelope glycoprotein of human immunodeficiency virus type 1 (HIV-1) is a dominant target against which the host's humoral immune response is directed. Unfortunately, gp120 proteins from different isolates of HIV are antigenically distinct, complicating the use of the envelope glycoprotein in vaccines designed to prevent acquired immunodeficiency syndrome. Using an enzyme-linked immunosorbent spot assay (ELISA), BALB/c mice immunized and boosted with recombinant purified gp120 were studied at the single cell level for their humoral immune response to HIV-1 envelope proteins. Approximately 90% of responding B cells produced antibodies reactive with the immunizing form of gp120 but not with gp120s from other strains of HIV. A novel sandwich ELISA was then used to analyze the frequency with which individual in vivo activated B cells produced antibodies that crossreacted with heterologous gp120s. Repeated immunizations with a single gp120 or with a mixture of different gp120s resulted in the activation of primarily mono-specific (noncrossreactive) B cells. In contrast, the sequential immunization of mice with recombinant purified envelope proteins from different strains of HIV (IIIB, SF2, and Zr6) induced the selective expansion of B cells producing highly crossreactive antibodies.

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Diagnosis of Secondary Syphilis Through Oral Lesions in a Patient Without Human Immunodeficiency Virus Infection

Oral Surgery Oral Medicine Oral Pathology and Oral Radiology ◽

10.1016/j.oooo.2013.12.016 ◽

2014 ◽

Vol 117 (2) ◽

pp. e153

Author(s):

BRUNA LAVINAS SAYED PICCIANI ◽

VANESSA DE CARLA BATISTA DOS SANTOS ◽

FELIPE LEAL MANHÃES DE SÁ ◽

DANIELA OTERO ◽

KARIN GONÇALVES SOARES CUNHA ◽

...

Keyword(s):

Human Immunodeficiency Virus ◽

Virus Infection ◽

Human Immunodeficiency Virus Infection ◽

Secondary Syphilis ◽

Oral Lesions ◽

Immunodeficiency Virus

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Typhoid Fever and Asymptomatic Human Immunodeficiency Virus Infection

Journal of Clinical Gastroenterology ◽

10.1097/00004836-199710000-00005 ◽

1997 ◽

Vol 25 (3) ◽

pp. 507-512 ◽

Cited By ~ 4

Author(s):

Mohammad Khan ◽

Yacoob Coovadia ◽

Adriaan Willem Sturm

Keyword(s):

Human Immunodeficiency Virus ◽

Virus Infection ◽

Human Immunodeficiency Virus Infection ◽

Typhoid Fever ◽

Immunodeficiency Virus ◽

Asymptomatic Human Immunodeficiency Virus

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Altered small-intestinal permeability associated with diarrhoea in human-immunodeficiency-virus-infected Caucasian and African subjects

Clinical Science ◽

10.1042/cs0810327 ◽

1991 ◽

Vol 81 (3) ◽

pp. 327-334 ◽

Cited By ~ 46

Author(s):

M. S. Kapembwa ◽

S. C. Fleming ◽

N. Sewankambo ◽

D. Serwadda ◽

S. Lucas ◽

...

Keyword(s):

Human Immunodeficiency Virus ◽

Virus Infection ◽

Human Immunodeficiency Virus Infection ◽

Intestinal Permeability ◽

Amperometric Detection ◽

Immunodeficiency Virus ◽

Caucasian Patients ◽

Small Intestinal ◽

African Patients ◽

Asymptomatic Human Immunodeficiency Virus

1. Small-intestine integrity in Caucasian and African patients infected with human immunodeficiency virus was determined by measuring the permeation across the mucosa of two sugars, lactulose and mannitol. 2. The sugars were assayed by h.p.l.c. and pulsed amperometric detection in 6 h urine samples. Stool microscopy for enteropathogens was performed in all patients. 3. The ratio of lactulose to mannitol recovered in urine was increased in Caucasian and African patients with advanced human immunodeficiency virus infection. Asymptomatic human-immunodeficiency-virus-infected subjects had a normal lactulose/mannitol ratio. African patients with diarrhoea showed a twofold reduction in mannitol excretion. Such a change in mannitol absorption was not detected in Caucasian patients and occurred regardless of the presence of enteropathogens. 4. Altered small-intestinal permeability is associated with symptomatic diarrhoea in human immunodeficiency virus infection in both Caucasian and African patients.

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