Passive hemagglutination inhibition test for diagnosis of brown recluse spider bite envenomation

1989 ◽  
Vol 18 (4) ◽  
pp. 441 ◽  
Author(s):  
SM Barrett ◽  
M Romine-Jenkins ◽  
JP Campbell
Keyword(s):  
Hemagglutination Inhibition ◽  
Inhibition Test ◽  
Passive Hemagglutination ◽  
Hemagglutination Inhibition Test ◽  
Spider Bite

Passive hemagglutination inhibition test for diagnosis of brown recluse spider bite envenomation

1993 ◽  
Vol 39 (10) ◽  
pp. 2104-2107 ◽  
Author(s):  
S M Barrett ◽  
M Romine-Jenkins ◽  
K E Blick
Keyword(s):  
Hemagglutination Inhibition ◽  
Passive Hemagglutination ◽  
Treatment Trials ◽  
Hemagglutination Inhibition Test ◽  
Argiope Trifasciata ◽  
Spider Bite ◽  
Phidippus Audax ◽  
Loxosceles Reclusa

Abstract Our goal was to recreate a passive hemagglutination inhibition (PHAI) test to diagnose brown recluse spider (BRS; Loxosceles reclusa) bite envenomation for treatment trials. Guinea pigs received intradermal injections of concentrated spider venom from the following species: Loxosceles reclusa, Argiope aurantia, Argiope trifasciata, Phidippus audax, and Lycosa frondicola. Skin lesion exudate was collected and tested with the BRS venom PHAI assay. From 51 separate collections of exudate, test sensitivity was 90% as long as 3 days after venom injection. Specificity was 100% with venom from the other spider species listed above in vivo (7 test samples) and in vitro (5 test samples), as well as with random bacterial exudate with and without added serial dilutions of BRS venom (10 test samples). The test was reproducible over repetitive assays to within one 10-fold dilution. A positive PHAI test result could function as an entry criterion for BRS bite victims in human treatment trials.

Simple hemagglutination inhibition test for the diagnosis of toxoplasmosis.

1985 ◽  
Vol 21 (2) ◽  
pp. 180-183 ◽  
Author(s):  
G N Chang ◽  
J A Nemzek ◽  
J L Tjostem ◽  
D A Gabrielson
Keyword(s):  
Hemagglutination Inhibition ◽  
Inhibition Test ◽  
Hemagglutination Inhibition Test

scholarly journals HUMAN IMMUNITY TO THE MENINGOCOCCUS

1969 ◽  
Vol 129 (6) ◽  
pp. 1349-1365 ◽  
Author(s):  
Emil C. Gotschlich ◽  
Teh Yung Liu ◽  
Malcolm S. Artenstein
Keyword(s):  
Molecular Weight ◽  
Hemagglutination Inhibition ◽  
Neuraminic Acid ◽  
Purification Procedure ◽  
Main Constituent ◽  
Passive Hemagglutination ◽  
Hemagglutination Inhibition Test ◽  
Group A ◽  
Human Immunity ◽  
Group B

The group-specific polysaccharides of group A and group C meningococci have been isolated by a new procedure which employs the cationic detergent Cetavlon to precipitate these polysaccharides from the whole culture. The A and C polysaccharide prepared by this method are noteworthy because they are of high molecular weight. The main constituent of the A polysaccharide is N-acetyl, O-acetyl mannosamine phosphate; of the C polysaccharide N-acetyl, O-acetyl neuraminic acid. This purification procedure, when applied to cultures of group B organisms, yields a polysaccharide consisting primarily of N-acetyl neuraminic acid. A passive hemagglutination test developed to measure antibodies to the polysaccharides demonstrated the specificity of these antigens. Using a hemagglutination inhibition test, these antigens were again found to be group-specific, and this test could be used for serogrouping meningococcal isolates.

Sign in / Sign up

Export Citation Format

Share Document