140 Human thrombin and calcium bound factor Xa significantly shorten tPA-induced fibrin clot lysis via a neutralization of plasminogen activator inhibitor type 1 activity: Its enhancement by either heparin or vitronectin

1997 ◽  
Vol 11 ◽  
pp. 40
Author(s):  
T. Urano ◽  
N. Nagai ◽  
H. Ihara ◽  
Y. Takada ◽  
A. Takada
Keyword(s):  
Plasminogen Activator ◽  
Factor Xa ◽  
Plasminogen Activator Inhibitor ◽  
Fibrin Clot ◽  
Clot Lysis ◽  
Human Thrombin ◽  
Inhibitor Type ◽  
Activator Inhibitor Type ◽  
Activator Inhibitor

Human Thrombin and Calcium Bound Factor Xa Significantly Shorten tPA-Induced Fibrin Clot Lysis Time via Neutralization of Plasminogen Activator Inhibitor Type 1 Activity

1998 ◽  
Vol 80 (07) ◽  
pp. 161-166 ◽  
Author(s):  
Tetsumei Urano ◽  
Nobuo Nagai ◽  
Megumi Matsuura ◽  
Hayato Ihara ◽  
Yumiko Takada ◽  
...  
Keyword(s):  
Factor Xa ◽  
Plasminogen Activator Inhibitor ◽  
Fibrin Clot ◽  
Clot Lysis ◽  
Lysis Time ◽  
Inhibitor Type ◽  
Activator Inhibitor Type ◽  
Activator Inhibitor ◽  
Pai 1

SummaryEmploying a fibrin clot lysis assay, we reassessed the significance of the neutralization of plasminogen activator inhibitor type 1 (PAI-1) activity by thrombin and factor-Xa in fibrinolysis. When PAI-1 enriched fibrin clots were formed using increasing concentrations of thrombin (0.1, 0.5, 1.0 IU/ml), their lysis times became shorter (43.8 ± 4.9, 25.7 ± 3.7, 13.9 ± 0.8 h respectively). Times were shortened further by either heparin (43.9 ± 11.0, 12.1 ± 2.6, 3.6 ± 0.2 h respectively) or vitronectin (17.0 ± 1.6, 1.9 ± 0.7, 0.9 ± 0.0 h respectively). Factor-Xa together with Ca++ shortened the clot lysis time further. Fibrin autography revealed that both enzymes dose dependently interfered with complex formation between tPA and PAI-1, making large amounts of tPA remaining to be free form. The mechanism seems to play a role in the coagulation associated enhancement of fibrinolysis.

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