Effects of metal binding affinity on the chemical and thermal stability of site-directed mutants of rat oncomodulin

1998 ◽  
Vol 71 (2-3) ◽  
pp. 157-172 ◽  
Author(s):  
Lili Zheng ◽  
Christopher W.V Hogue ◽  
John D Brennan
Keyword(s):  
Thermal Stability ◽  
Binding Affinity ◽  
Metal Binding ◽  
Thermal Stability Of

Metal-binding hydrazone photoswitches for visible light reactivity and variable relaxation kinetics

2017 ◽  
Vol 16 (11) ◽  
pp. 1604-1612 ◽  
Author(s):  
Kacey C. Hall ◽  
Andrew T. Franks ◽  
Rory C. McAtee ◽  
Michael S. Wang ◽  
Vivian I. Lu ◽  
...  
Keyword(s):  
Thermal Stability ◽  
Visible Light ◽  
Binding Affinity ◽  
Blue Light ◽  
Metal Binding ◽  
Relaxation Kinetics ◽  
Photostationary State ◽  
State Composition

Photoactive aroylhydrazones demonstrate variability in UVA, UVC and blue light photoreactivity, photostationary state composition, photoisomer thermal stability, and relative iron(iii) binding affinity in ways that may inform metal-gated photoswitching applications.

scholarly journals Antigen-binding affinity and thermostability of chimeric mouse-chicken IgY and mouse-human IgG antibodies with identical variable domains

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Juho Choi ◽  
Minjae Kim ◽  
Joungmin Lee ◽  
Youngsil Seo ◽  
Yeonkyoung Ham ◽  
...  
Keyword(s):  
Thermal Stability ◽  
Binding Affinity ◽  
Antigen Binding ◽  
Chimeric Mouse ◽  
Binding Parameters ◽  
Igg Antibodies ◽  
Human Igg ◽  
Variable Domains ◽  
Thermal Stability Of ◽  
Parental Mouse

AbstractConstant (C)-region switching of heavy (H) and/or light (L) chains in antibodies (Abs) can affect their affinity and specificity, as demonstrated using mouse, human, and chimeric mouse-human (MH) Abs. However, the consequences of C-region switching between evolutionarily distinct mammalian and avian Abs remain unknown. To explore C-region switching in mouse-chicken (MC) Abs, we investigated antigen-binding parameters and thermal stability of chimeric MC-6C407 and MC-3D8 IgY Abs compared with parental mouse IgGs and chimeric MH Abs (MH-6C407 IgG and MH-3D8 IgG) bearing identical corresponding variable (V) regions. The two MC-IgYs exhibited differences in antigen-binding parameters and thermal stability from their parental mouse Abs. However, changes were similar to or less than those between chimeric MH Abs and their parental mouse Abs. The results demonstrate that mammalian and avian Abs share compatible V-C region interfaces, which may be conducive for the design and utilization of mammalian-avian chimeric Abs.

Effects of estradiol and 4-hydroxytamoxifen on the conformation, thermal stability, and DNA recognition of estrogen receptor β

2007 ◽  
Vol 85 (1) ◽  
pp. 1-10 ◽  
Author(s):  
Veena Vijayanathan ◽  
Norma J. Greenfield ◽  
T. J. Thomas ◽  
Margarita M. Ivanova ◽  
Valentyn V. Tyulmenkov ◽  
...  
Keyword(s):  
Thermal Stability ◽  
Binding Affinity ◽  
Dna Recognition ◽  
Thermal Unfolding ◽  
Estrogen Response Element ◽  
Cd Spectrum ◽  
Estrogen Response ◽  
Helical Content ◽  
Partial Unfolding ◽  
Thermal Stability Of

Estrogen receptors (ERα and ERβ) are ligand-activated transcription factors. We examined the effects of estradiol (E2), 4-hydroxytamoxifen (HT), and the estrogen response element (ERE) on the helical content and thermal unfolding of ERβ. A circular dichroism (CD) spectrum of ERβ showed changes at 210 and 222 nm that were due to the presence of E2, which is indicative of partial unfolding. In contrast, HT did not alter the CD spectrum of ERβ. The addition of E2 + ERE caused an increase in the α-helical content and an increase in the temperature midpoint of folding transition (TM) from 39 ± 0.7 °C to 57.2 ± 1 °C. The addition of E2 + mutant ERE, or E2 + control oligonucleotide, increased the TM of ERβ to 45 ± 2 °C only. In the presence of HT, ERβ yielded similar TM values (55–58 °C) with ERE, mutant ERE, or control oligodeoxynucleotide. The binding affinity of ERβ for ERE increased 125.7-fold as a result of the presence of E2, but only 4-fold as a result of HT. These results demonstrate coupled effects of E2 and ERE on ERβ stability and binding affinity. The increased thermal stability of HT–ERβ–ERE was associated with reduced specificity of ERβ–ERE recognition, illustrating profound differences in conformational states of ERβ induced by E2 and HT.

The influence of acylation, metal binding and natural antioxidants on the thermal stability of red cabbage anthocyanins in neutral solution

2019 ◽  
Vol 10 (10) ◽  
pp. 6740-6751 ◽  
Author(s):  
Julie-Anne Fenger ◽  
Micheal Moloney ◽  
Rebecca J. Robbins ◽  
Thomas M. Collins ◽  
Olivier Dangles
Keyword(s):  
Thermal Stability ◽  
Metal Binding ◽  
Natural Antioxidants ◽  
Neutral Solution ◽  
Red Cabbage ◽  
Π Stacking ◽  
Thermal Stability Of

For diacylated anthocyanins, π-stacking (vertical) interactions + (horizontal) metal binding modulate color and greatly enhance its stability in neutral solution

The Ordered Phase Formation in Ti-Al-Ga Alloys

1970 ◽  
Vol 28 ◽  
pp. 440-441
Author(s):  
Shiro Fujishiro ◽  
Harold L. Gegel
Keyword(s):  
Thermal Stability ◽  
High Temperature ◽  
Titanium Alloys ◽  
Growth Kinetics ◽  
Stoichiometric Composition ◽  
Good Potential ◽  
Alpha Titanium ◽  
Cold Rolled ◽  
Kinetics Of ◽  
Thermal Stability Of

Ordered-alpha titanium alloys having a DO19 type structure have good potential for high temperature (600°C) applications, due to the thermal stability of the ordered phase and the inherent resistance to recrystallization of these alloys. Five different Ti-Al-Ga alloys consisting of equal atomic percents of aluminum and gallium solute additions up to the stoichiometric composition, Ti3(Al, Ga), were used to study the growth kinetics of the ordered phase and the nature of its interface.The alloys were homogenized in the beta region in a vacuum of about 5×10-7 torr, furnace cooled; reheated in air to 50°C below the alpha transus for hot working. The alloys were subsequently acid cleaned, annealed in vacuo, and cold rolled to about. 050 inch prior to additional homogenization

Microstructure studies of tungsten silicide schottky contacts on Gaas

1987 ◽  
Vol 45 ◽  
pp. 328-329
Author(s):  
Yih-Cheng Shih ◽  
E. L. Wilkie
Keyword(s):  
Thermal Stability ◽  
Field Effect Transistors ◽  
Schottky Contact ◽  
Schottky Contacts ◽  
Excellent Thermal Stability ◽  
Barrier Heights ◽  
Gaas Substrates ◽  
Film Adhesion ◽  
Threshold Voltages ◽  
Thermal Stability Of

Tungsten silicides (WSix) have been successfully used as the gate materials in self-aligned GaAs metal-semiconductor-field- effect transistors (MESFET). Thermal stability of the WSix/GaAs Schottky contact is of major concern since the n+ implanted source/drain regions must be annealed at high temperatures (∼ 800°C). WSi0.6 was considered the best composition to achieve good device performance due to its low stress and excellent thermal stability of the WSix/GaAs interface. The film adhesion and the uniformity in barrier heights and ideality factors of the WSi0.6 films have been improved by depositing a thin layer of pure W as the first layer on GaAs prior to WSi0.6 deposition. Recently WSi0.1 has been used successfully as the gate material in 1x10 μm GaAs FET's on the GaAs substrates which were sputter-cleaned prior to deposition. These GaAs FET's exhibited uniform threshold voltages across a 51 mm wafer with good film adhesion after annealing at 800°C for 10 min.

Thermal stability of Al-Cu-Fe icosahedral alloys

1991 ◽  
Vol 1 (12) ◽  
pp. 1823-1836 ◽  
Author(s):  
M. Bessière ◽  
A. Quivy ◽  
S. Lefebvre ◽  
J. Devaud-Rzepski ◽  
Y. Calvayrac
Keyword(s):  
Thermal Stability ◽  
Thermal Stability Of

Practical criteria for the thermal stability of a unidimensional superconductor

1994 ◽  
Vol 4 (4) ◽  
pp. 653-657
Author(s):  
B. Bonzi ◽  
M. El Khomssi ◽  
H. Lanchon-Ducauquis
Keyword(s):  
Thermal Stability ◽  
Thermal Stability Of
Sign in / Sign up

Export Citation Format

Share Document