Cytophotometric DNA-Measurements in Abortion

1978 ◽  
Vol 162 (3) ◽  
pp. 253-262 ◽  
Author(s):  
W.-P. Kunze
Keyword(s):  
Dna Measurements

DNA Content of Normal and Neoplastic Urothelial Cells

1980 ◽  
Vol 66 (4) ◽  
pp. 445-458 ◽  
Author(s):  
Mathilde E. Boon
Keyword(s):  
Dna Content ◽  
Cell Populations ◽  
Malignant Cells ◽  
Urothelial Cells ◽  
Chromatin Pattern ◽  
Normal Bladder ◽  
Well Differentiated ◽  
Dna Measurements ◽  
Combined Parameters ◽  
Umbrella Cells

In search for suitable parameters to detect neoplastic urothelial cells in Acriflavine-Feulgen-SITS stained specimen we compared the cytofluorometric DNA content with the morphology of normal urothelial cells (bladder scrapings) and neoplastic urothelial cells from grade 1, 2, 3 and 4 tumors. An individual normal urothelial cell could not be distinguished from a grade 1 tumor cell, neither morphologically nor fluorometrically. However, the shape of the histograms of DNA measurements of the cell populations of respectively normal bladder scrapings and grade 1 tumors differs. It is postulated that also morphometry of these cell populations may be of some aid to distinguish well-differentiated neoplastic cells from normal urothelial cells. Seventy-one percent of the morphologically malignant cells in the grade 2, 3 and 4 tumor samples could be identified by applying the combined parameters: high DNA content (> 5 C) and nuclear-cytoplasmic ratio (> 0.5) and all grade 2, 3 and 4 tumor samples contained cells which were objectively classified as malignant. Using the same parameters morphologically malignant cells could be distinguished from normal, polyploid umbrella cells, thus these malignant cells are detectable objectively without using chromatin pattern as parameter.

DNA measurements in chronic hepatitis, cirrhosis and hepatocellular carcinoma

2008 ◽  
Vol 10 (5) ◽  
pp. 313-318 ◽  
Author(s):  
Hsien-Hong Lin ◽  
Wei-Chue Shyu ◽  
Gu-Ling Chen ◽  
Yn-Huei Lin ◽  
Tong-Jong Chen ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Chronic Hepatitis ◽  
Dna Measurements

DNA measurements for monitoring chemotherapy in advanced head and neck carcinomas

Head & Neck ◽  
1994 ◽  
Vol 16 (3) ◽  
pp. 240-245 ◽  
Author(s):  
Hans J. Welkoborsky ◽  
Wolf J. Mann ◽  
Cem Sanal ◽  
James E. Freije
Keyword(s):  
Head And Neck ◽  
Advanced Head ◽  
Head And Neck Carcinomas ◽  
Dna Measurements

Flow cytometric DNA measurements in paraffin-embedded bladder carcinoma tissue before and after precystectomy radiotherapy

1987 ◽  
Vol 10 (2) ◽  
pp. 149-155 ◽  
Author(s):  
A.B. Jacobsen ◽  
S.D. Foss ◽  
S. Lunde ◽  
J.E. Melvik ◽  
E.O. Pettersen
Keyword(s):  
Bladder Carcinoma ◽  
Carcinoma Tissue ◽  
Flow Cytometric ◽  
Before And After ◽  
Dna Measurements

scholarly journals The effect of splenectomy on platelet formation and megakaryocyte DNA content in rats

Blood ◽  
1984 ◽  
Vol 63 (3) ◽  
pp. 593-597 ◽  
Author(s):  
G Tanum ◽  
A Sonstevold ◽  
E Jakobsen
Keyword(s):  
Bone Marrow ◽  
Dna Content ◽  
Direct Action ◽  
Surgical Trauma ◽  
Sham Operation ◽  
Acetylcholinesterase Staining ◽  
Effect Of Splenectomy ◽  
A Minor ◽  
Dna Measurements ◽  
Platelet Formation

Abstract The DNA content of rat bone marrow megakaryocytes (MK) was studied by Feulgen photometry following splenectomy and sham operation, respectively. The DNA measurements were preceded by acetylcholinesterase staining for identification of the 2N-8N MK. The number of 2N-8N MK decreased to minimum values, while the number of 16N- 64N MK increased to maximum values about 4 days following both splenectomy and sham operation. However, the changes were somewhat more pronounced following splenectomy than sham operation. The total MK number did not change significantly. Platelet production, measured by 35S incorporation into platelets, increased during the first 2 days and remained high for 6–7 days, increasing the platelet counts. All values were about normal 30 days after surgery, except for a minor thrombocytosis following splenectomy. The early, highly significant thrombocytosis, following both splenectomy and general surgery, is caused by increased production of platelets due to the surgical trauma. This is caused by a direct action on bone marrow MK by transforming 2N- 8N MK into higher ploidy classes. Lack of splenic platelet pooling may influence the grade and duration of the early thrombocytosis after splenectomy. The late, long-lasting, minor thrombocytosis, which occurs after splenectomy but not after sham operation, can be explained by the removal of the splenic platelet pool.

scholarly journals Intracellular pH, esterase activity, and DNA measurements of human lung carcinomas by flow cytometry

Cytometry ◽  
1990 ◽  
Vol 11 (3) ◽  
pp. 341-348 ◽  
Author(s):  
F. Liewald ◽  
N. Demmel ◽  
R. Wirsching ◽  
H. Kahle ◽  
G. Valet
Keyword(s):  
Flow Cytometry ◽  
Intracellular Ph ◽  
Esterase Activity ◽  
Human Lung ◽  
Lung Carcinomas ◽  
Dna Measurements

scholarly journals Micropropagation and Cellular Behaviour Changes During in vitro Flowering of Impatiens balsamina

2019 ◽  
Vol 37 ◽  
Author(s):  
N. MOHAMED ◽  
R.M. TAHA ◽  
U.N.A.A. RAZAK
Keyword(s):  
Chromosome Count ◽  
In Vitro Flowering ◽  
Root Cells ◽  
Ploidy Levels ◽  
Impatiens Balsamina ◽  
Cellular Behaviour ◽  
Behaviour Changes ◽  
Dna Measurements

ABSTRACT: In this study, the micropropagation of Impatiens balsamina was established from stem and shoot explants. The effects of GA3 and glutathione on the morphogenesis of this species were also investigated, in order to induce in vitro flowering. It was found that the optimum in vitro plant regeneration was achieved on MS medium supplemented with 1.0 mg L-1 GA3 and in vitro flowering was also obtained from the same medium after 4 weeks of culture. To understand cellular behavior during in vitro flowering, Mitotic Index (MI), chromosome counts, measurement of mean cell and nuclear areas, DNA measurements and ploidy levels were analyzed from in vivo plants, in vitro grown plants and plantlets that flowered in vitro. The chromosome count was the same for all, 2x=2n=14 or n=7. However, it was observed that in vitro flowering plants of Impatiens balsamina had the highest percentage of polyploid cells (30.7%), based on a histogram plotted by the AxioVision 4.7 software. It was found that plant growth regulators, especially GA3, increased the polyploidy level of the meristematic root cells.

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