Experimental infection of cattle with several Borrelia burgdorferi sensu lato strains; immunological heterogeneity of strains as revealed in serological tests

1998 ◽  
Vol 60 (1) ◽  
pp. 27-43 ◽  
Author(s):  
J Tuomi ◽  
L.K Rantamäki ◽  
R Tanskanen
Keyword(s):  
Borrelia Burgdorferi ◽  
Experimental Infection ◽  
Borrelia Burgdorferi Sensu Lato ◽  
Serological Tests ◽  
Immunological Heterogeneity

scholarly journals Association between Sudden Sensorineural Hearing Loss and Lyme Disease

2021 ◽  
Vol 10 (5) ◽  
pp. 1130
Author(s):  
Klaudia Sowula ◽  
Joanna Szaleniec ◽  
Kamila Stolcman ◽  
Piotr Ceranowicz ◽  
Sebastian Kocoń ◽  
...  
Keyword(s):  
Hearing Loss ◽  
Lyme Disease ◽  
Borrelia Burgdorferi ◽  
Sensorineural Hearing Loss ◽  
Sudden Sensorineural Hearing Loss ◽  
Sensorineural Hearing ◽  
Borrelia Burgdorferi Sensu Lato ◽  
Complete Regression ◽  
Serological Tests ◽  
Possible Cause

Objectives: Sudden sensorineural hearing loss (SSNHL) is defined as sensorineural hearing loss of 30 dB or more over at least three adjacent audiometric frequencies occurring within a 72-h period of time. One of the causes of SSNHL could be the progressive inflammatory state caused by an infection. The aim of this study was to assess the prevalence of SSNHL caused by various factors, most importantly those potentially related to Lyme disease. Material and Methods: The study includes a group of 86 patients between the ages of 20 and 70 who were hospitalized due to SSNHL between 2017 and 2018. All of these patients underwent a detailed medical interview and an otolaryngological examination, including audiological and diagnostic tests. Additionally, ELISA and Western blot tests were performed to confirm the diagnosis of Lyme disease. Results: In this group of 86 patients, nine patients presented with positive antibodies toward Borrelia burgdorferi sensu lato. This group was treated with antibiotics and experienced partial or complete regression of their deafness. This may suggest a relationship between SSNHL and Lyme disease. Conclusion: Infections caused by Borrelia burgdorferi may contribute to the development of inflammatory and angiopathic lesions, which are a possible cause of SSNHL. The longer the duration of the infection, the greater the likelihood of permanent and irreversible changes in the vessels of the cochlea or auditory nerve. Therefore, serological tests for Borrelia burgdorferi should be performed during the diagnosis of SSNHL as a possible cause of this illness.

scholarly journals Impact of Strain Heterogeneity on Lyme Disease Serology in Europe: Comparison of Enzyme-Linked Immunosorbent Assays Using Different Species of Borrelia burgdorferi Sensu Lato

1998 ◽  
Vol 36 (2) ◽  
pp. 427-436 ◽  
Author(s):  
Ulrike Hauser ◽  
Heide Krahl ◽  
Helmut Peters ◽  
Volker Fingerle ◽  
Bettina Wilske
Keyword(s):  
Borrelia Burgdorferi ◽  
Blood Donors ◽  
Surface Protein ◽  
Sensu Stricto ◽  
Immunoglobulin M ◽  
Borrelia Burgdorferi Sensu Lato ◽  
Serological Tests ◽  
Western Blots ◽  
Previous Infection ◽  
Immunosorbent Assays

For the standardization of serological tests for Lyme borreliosis (LB) in Europe, the influence of the heterogeneity of Borrelia burgdorferi sensu lato must be assessed in detail. For this study four immunoglobulin M (IgM) and IgG enzyme-linked immunosorbent assays (ELISAs) with octyl-β-d-glucopyranoside extracts of strains PKo (Borrelia afzelii), PBi (Borrelia garinii), and PKa2 and B31 (both B. burgdorferi sensu stricto) were compared. Strains PKo, PBi, and PKa2 at the passages used for antigen preparations abundantly expressed outer surface protein C (OspC), whereas strain B31 at the passage used for antigen preparation did not express OspC. Sera (all from Germany) from 222 patients with clinically defined LB of all stages, 133 blood donors, and 458 forest workers were tested. None of the forest workers had symptoms consistent with LB at the time that the samples were collected. For IgM tests, receiver operating characteristic curves demonstrated that discrimination between sera from patients and blood donors was best with strain PKo and worst with strain B31. The discriminatory abilities of the four IgG ELISAs were similar in a diagnostically reasonable specificity range (90 to 100%). More than 20% of the sera from forest workers reacted strongly in the PKo IgG ELISA (optical density value, >1.5; other assays, less than 8%). Western blots of the sera with the most discrepant ELISA results revealed almost exclusive reactivity with p17. This highly immunogenic antigen is only expressed by strain PKo. This observation might be important for the development of assays enabling discrimination between asymptomatic or previous infection and active disease.

scholarly journals LYME BORRELIOSIS IN NORTH BACKA DISTRICT

2017 ◽  
Vol 10 (2) ◽  
pp. 81-94
Author(s):  
Dragica Kovačević Berić ◽  
Tibor Molnar ◽  
Morana Miković ◽  
Sara Savić
Keyword(s):  
Lyme Disease ◽  
Borrelia Burgdorferi ◽  
Average Rate ◽  
Borrelia Burgdorferi Sensu Lato ◽  
Prevalence Rates ◽  
Serological Tests ◽  
Causative Agents ◽  
Vector Borne ◽  
Epidemiological Characteristics ◽  
Moderate Climate

Lyme disease is the most common vector borne disease in regions with moderate climate. The cause of the disease is Borrelia burgdorferi sensu lato (B. burgdorferi s.l.), whereas infection is spread via bite from a tick carrying the causative agent. The objective of this work is the examination of descriptive-epidemiological characteristics of Lyme disease in North Backa region in the period from 2012 to 2016. In terms of classifi cation, Lyme dis-ease does not fall into the category of diseases that must be reported when infection occurs among animals. The average rate of incidence in humans during the observed five-year period is 7/100.000. Th e highest incidence of this disease among humans was observed in 2013 (In 12.2/100,000) with 22 reported cases, whereas the lowest incidence was in 2015 (In 5.0/100,000) with 9 reported cases. The highest rate of incidence was recorded in Bačka Topola municipality – 15.9/100.000; followed by Mali Iđoš with 7.2/100.000; while the lowest rate was recorded in Subotica being 5.0 cases per 100,000 residents. Th e presence of the disease was confi rmed in all patients via labo-ratory (serological) tests. Women were more likely to become infected, with the male-female patient ratio being 41%-59%. The disease was documented predominantly among people aged 50-69. The majority of cases were re-corded during spring and summer, in the period from May to June (71%). Among animals, the disease was documented in Subotica and Mali Iđoš municipality, with average prevalence rates among dogs and horses being 0.07%; and 1.52% respectively. Subotica municipality has the most accurate records of cases and a prevalence of 0.06% among dogs, and 1.43% among horses, while Mali Iđoš municipality has higher prevalence, being 2.99% among horses and 0.14% among dogs. The highest occurrence of Lyme disease among animals was documented in 2015, with a prevalence of 2.85% among horses. The presence of causative agents among examined ticks in Mali Iđoš municipality was 16.28% on average, predominantly in 2015 (in 21.21% of examined ticks).

scholarly journals A non-traumatic, blue-purple auricle: case report

2009 ◽  
Vol 124 (7) ◽  
pp. 804-806 ◽  
Author(s):  
A Sewnaik ◽  
J Kamarashev ◽  
N S Peters ◽  
J B van Rijswijk
Keyword(s):  
Case Report ◽  
Lyme Disease ◽  
Literature Review ◽  
Borrelia Burgdorferi ◽  
Northern Hemisphere ◽  
Typical Case ◽  
Borrelia Burgdorferi Sensu Lato ◽  
Diagnostic Process ◽  
Serological Tests ◽  
Tick Borne Disease

AbstractObjective:We report a typical case of earlobe lymphocytoma.Method:A case report and literature review are presented.Results:A 10-year-old girl presented with a blue-coloured earlobe. A diagnosis of Lyme disease was confirmed by serological tests. Lyme borreliosis is the most common tick-borne disease in the northern hemisphere. It is caused by the spirochete Borrelia burgdorferi sensu lato. The patient was successfully treated with antibiotics.Conclusion:The diagnostic process and ENT symptomatology of Lyme disease and borrelial lymphocytoma are summarised and discussed.

scholarly journals Serological Evidence of Natural Exposure to Tick-Borne Pathogens in Horses, Romania

2021 ◽  
Vol 9 (2) ◽  
pp. 373
Author(s):  
Andreea Monica Bogdan ◽  
Mariana Ionita ◽  
Ioan Liviu Mitrea
Keyword(s):  
Confidence Interval ◽  
Borrelia Burgdorferi ◽  
Anaplasma Phagocytophilum ◽  
Borrelia Burgdorferi Sensu Lato ◽  
Serological Evidence ◽  
North Central ◽  
Natural Exposure

The purpose of this study was to investigate the seroprevalence of selected tick-borne-pathogens (TBPs) among Romanian horses. For this, a total of 223 animals originating from north, central, and southeast Romania, including horses from stud farms (n = 118) and working horses (n = 105), were tested using a commercial rapid ELISA-based test. Overall, 10.3% (95% confidence interval (CI): 6.7–15.1%) of the tested horses were seropositive for antibodies (Ab) against Anaplasma phagocytophilum. Additionally, 18.8% (95% CI: 13.9–24.6%) and 0.5% (95% CI: 0.01–2.5%) of horses were seropositive for Ab against Borrelia burgdorferi sensu lato and Ehrlichia spp., respectively. Among the tested horses, 3.1% were seroreactive to two or three pathogens. These findings show the natural exposure of Romanian horses to zoonotic tick-borne pathogens and emphasize the need for further studies to better understand the epidemiology of equine tick-borne diseases in Romania.

scholarly journals Surveillance of Ticks and Tick-Borne Pathogens in Suburban Natural Habitats of Central Maryland

2021 ◽  
Author(s):  
Matthew T Milholland ◽  
Lars Eisen ◽  
Robyn M Nadolny ◽  
Andrias Hojgaard ◽  
Erika T Machtinger ◽  
...  
Keyword(s):  
Lyme Disease ◽  
Borrelia Burgdorferi ◽  
Spotted Fever ◽  
Babesia Microti ◽  
Borrelia Burgdorferi Sensu Lato ◽  
Spotted Fever Group ◽  
Borrelia Miyamotoi ◽  
Natural Habitats ◽  
Questing Ticks ◽  
Tick Vectors

Abstract Lyme and other tick-borne diseases are increasing in the eastern United States and there is a lack of research on integrated strategies to control tick vectors. Here we present results of a study on tick-borne pathogens detected from tick vectors and rodent reservoirs from an ongoing 5-yr tick suppression study in the Lyme disease-endemic state of Maryland, where human-biting tick species, including Ixodes scapularis Say (Acari: Ixodidae) (the primary vector of Lyme disease spirochetes), are abundant. During the 2017 tick season, we collected 207 questing ticks and 602 ticks recovered from 327 mice (Peromyscus spp. (Rodentia: Cricetidae)), together with blood and ear tissue from the mice, at seven suburban parks in Howard County. Ticks were selectively tested for the presence of the causative agents of Lyme disease (Borrelia burgdorferi sensu lato [s.l.]), anaplasmosis (Anaplasma phagocytophilum), babesiosis (Babesia microti), ehrlichiosis (Ehrlichia ewingii, Ehrlichia chaffeensis, and ‘Panola Mountain’ Ehrlichia) and spotted fever group rickettsiosis (Rickettsia spp.). Peromyscus ear tissue and blood samples were tested for Bo. burgdorferi sensu stricto (s.s), A. phagocytophilum, Ba. microti, and Borrelia miyamotoi. We found 13.6% (15/110) of questing I. scapularis nymphs to be Bo. burgdorferi s.l. positive and 1.8% (2/110) were A. phagocytophilum positive among all sites. Borrelia burgdorferi s.s. was found in 71.1% (54/76) of I. scapularis nymphs removed from mice and 58.8% (194/330) of captured mice. Results from study on tick abundance and pathogen infection status in questing ticks, rodent reservoirs, and ticks feeding on Peromyscus spp. will aid efficacy evaluation of the integrated tick management measures being implemented.

scholarly journals Seropositivity to canine tick-borne pathogens in a population of sick dogs in Italy

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Jairo Alfonso Mendoza-Roldan ◽  
Giovanni Benelli ◽  
Marcos Antonio Bezerra-Santos ◽  
Viet-Linh Nguyen ◽  
Giuseppe Conte ◽  
...  
Keyword(s):  
Borrelia Burgdorferi ◽  
Serological Test ◽  
Control Strategies ◽  
Central Italy ◽  
Rickettsia Conorii ◽  
High Seroprevalence ◽  
The South ◽  
Serum Samples ◽  
Serological Tests ◽  
Distribution Maps

Abstract Background Canine vector-borne diseases (CVBDs) associated to ticks are among the most important health issues affecting dogs. In Italy, Ehrlichia canis, Anaplasma spp., Rickettsia conorii and Borrelia burgdorferi (s.l.) have been studied in both healthy canine populations and those clinically ill with suspected CVBDs. However, little information is currently available on the overall prevalence and distribution of these pathogens in the country. The aim of this study was to assess the prevalence and distribution of tick-borne pathogens (TBPs) in clinically suspect dogs from three Italian macro areas during a 15-year period (2006–2020). Methods A large dataset (n = 21,992) of serological test results for selected TBPs in three macro areas in Italy was analysed using a Chi-square test to evaluate the associations between the categorical factors (i.e. macro area, region, year, sex and age) and a standard logistic regression model (significance set at P = 0.05). Serological data were presented as annual and cumulative prevalence, and distribution maps of cumulative positive cases for TBPs were generated. Results Of the tested serum samples, 86.9% originated from northern (43.9%) and central (43%) Italy. The majority of the tests was requested for the diagnosis of E. canis (47%; n = 10,334), followed by Rickettsia spp. (35.1%; n = 7725), B. burgdorferi (s.l.) (11.6%; n = 2560) and Anaplasma spp. (6.2%; n = 1373). The highest serological exposure was recorded for B. burgdorferi (s.l.) (83.5%), followed by Rickettsia spp. (64.9%), Anaplasma spp. (39.8%) and E. canis (28.7%). The highest number of cumulative cases of Borrelia burgdorferi (s.l.) was recorded in samples from Tuscany, central Italy. Rickettsia spp. was more prevalent in the south and on the islands, particularly in dogs on Sicily older than 6 years, whereas Anaplasma spp. was more prevalent in the north and E. canis more prevalent in the south and on the islands. Conclusions The results of this study highlight the high seroprevalence and wide distribution of the four TBPs in dogs with clinically suspected CVBDs from the studied regions of Italy. The very high seroprevalence of B. burgdorferi (s.l.) exemplifies a limitation of this study, given the use of clinically suspect dogs and the possibility of cross-reactions when using serological tests. The present research provides updated and illustrative information on the seroprevalence and distribution of four key TBPs, and advocates for integrative control strategies for their prevention. Grapic abstract

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