Simultaneous determination of creatinine, hypoxanthine and uric acid in biological samples by column-switching liquid chromatography with ultraviolet detection

1997 ◽  
Vol 15 (9-10) ◽  
pp. 1621-1626 ◽  
Author(s):  
Tokuichiro Seki ◽  
Yoshimasa Orita ◽  
Kenji Yamaji ◽  
Akiko Shinoda
Keyword(s):  
Uric Acid ◽  
Liquid Chromatography ◽  
Simultaneous Determination ◽  
Biological Samples ◽  
Column Switching ◽  
Ultraviolet Detection

Deproteinizing methods evaluated for determination of uric acid in serum by reversed-phase liquid chromatography with ultraviolet detection.

1987 ◽  
Vol 33 (8) ◽  
pp. 1427-1430 ◽  
Author(s):  
R Sakuma ◽  
T Nishina ◽  
M Kitamura
Keyword(s):  
Uric Acid ◽  
Liquid Chromatography ◽  
Perchloric Acid ◽  
High Performance ◽  
Reversed Phase ◽  
Precipitation Method ◽  
Zinc Hydroxide ◽  
Good Precision ◽  
Ultraviolet Detection

Abstract We evaluated six deproteinizing methods for determination of uric acid in serum by "high-performance" liquid chromatography with ultraviolet detection: those involving zinc hydroxide, sodium tungstate, trichloroacetic acid, perchloric acid, acetonitrile, and centrifugal ultrafiltration (with Amicon MPS-1 devices). We used a Toyosoda ODS-120A reversed-phase column. The mobile phase was sodium phosphate buffer (40 mmol/L, pH 2.2) containing 20 mL of methanol per liter. Absorbance of the eluate was monitored at 284 nm. The precipitation method with perchloric acid gave high recoveries of uric acid and good precision, and results agreed with those by the uricase-catalase method of Kageyama (Clin Chim Acta 1971;31:421-6).

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