Determination of anti–Toxoplasma gondii immunoglobulin G avidity: adaptation to the vidas system (bioMérieux)

1998 ◽  
Vol 32 (2) ◽  
pp. 69-73 ◽  
Author(s):  
Hervé Pelloux ◽  
Emmanuelle Brun ◽  
Guy Vernet ◽  
Suzanne Marcillat ◽  
Michel Jolivet ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Immunoglobulin G

Determination of the Viability of Toxoplasma gondii in Cured Ham Using Bioassay: Influence of Technological Processing and Food Safety Implications

2010 ◽  
Vol 73 (12) ◽  
pp. 2239-2243 ◽  
Author(s):  
SUSANA BAYARRI ◽  
MARÍA J. GRACIA ◽  
REGINA LÁZARO ◽  
CONSUELO PÉREZ-ARQUILLUÉ ◽  
MONTSERRAT BARBERÁN ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Horizontal Transmission ◽  
Meat Product ◽  
Immunofluorescence Assay ◽  
Nitrite Concentration ◽  
Bioassay Technique ◽  
Technological Processing ◽  
Undercooked Meat ◽  
Porcine Tissues

Toxoplasmosis is a zoonotic disease caused by the protozoan Toxoplasma gondii and distributed worldwide. Ingestion of viable cysts from infected raw or undercooked meat is an important route of horizontal transmission of the parasite to humans. Little information is available concerning the effect of commercial curing on cysts of T. gondii. This study is the first in which the influence of processing of cured ham on the viability of T. gondii has been evaluated, using bioassay to assess the risk of infection from eating this meat product. Naturally infected pigs were selected for the study, and a mouse concentration bioassay technique was used to demonstrate viable bradyzoites of T. gondii in porcine tissues and hams. No viable parasites were found in the final product (14 months of curing) based on results of the indirect immunofluorescence assay and histological and PCR analyses. Our results indicate that the consumption of hams cured as described here poses an insignificant risk of acquiring toxoplasmosis. However, additional studies are required to evaluate the safety of ham products cured under different conditions of curing time, salt, and nitrite concentration.

scholarly journals Cervical Cat Scratch Disease Lymphadenitis in a Patient with Immunoglobulin M Antibodies to Toxoplasma gondii

2002 ◽  
Vol 9 (2) ◽  
pp. 496-498
Author(s):  
Mardjan Arvand ◽  
Ilkay Kazak ◽  
Sergije Jovanovic ◽  
Hans-Dieter Foss ◽  
Oliver Liesenfeld
Keyword(s):  
Toxoplasma Gondii ◽  
Clinical Symptoms ◽  
Bartonella Henselae ◽  
Cervical Lymphadenopathy ◽  
Immunoglobulin M ◽  
Cat Scratch Disease ◽  
Specific Immunoglobulin ◽  
Acute Toxoplasmosis

ABSTRACT We report on a young patient with chronic cervical lymphadenopathy and serological and histological evidence for infection with Bartonella henselae and Toxoplasma gondii. Serological follow-up studies, including testing for avidity of Toxoplasma-specific immunoglobulin G antibodies, assisted in the determination of the cause of the acute lymphadenitis. Our results suggest that the clinical symptoms were most likely due to cat scratch disease rather than to acute toxoplasmosis.

Usefulness of Toxoplasma gondii recombinant antigens (GRA1, GRA7 and SAG1) in an immunoglobulin G avidity test for the serodiagnosis of toxoplasmosis

2006 ◽  
Vol 100 (2) ◽  
pp. 333-337 ◽  
Author(s):  
H. Pietkiewicz ◽  
E. Hiszczyńska-Sawicka ◽  
J. Kur ◽  
E. Petersen ◽  
H. V. Nielsen ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Immunoglobulin G ◽  
Recombinant Antigens ◽  
Avidity Test

scholarly journals Determination of Chemical Inhibitor Efficiency against Intracellular Toxoplasma Gondii Growth Using a Luciferase-Based Growth Assay

10.3791/60985 ◽  
2020 ◽  
Author(s):  
Melanie Key ◽  
Amy Bergmann ◽  
Chiara Micchelli ◽  
L. Brock Thornton ◽  
Sophie Millard ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Inhibitor Efficiency ◽  
Growth Assay

Automated particle-counting immunoassay for alpha-fetoprotein.

1981 ◽  
Vol 27 (1) ◽  
pp. 64-67 ◽  
Author(s):  
D Collet-Cassart ◽  
C G Magnusson ◽  
J G Ratcliffe ◽  
C L Cambiaso ◽  
P L Masson
Keyword(s):  
Correlation Coefficient ◽  
Immunoglobulin G ◽  
Incubation Time ◽  
Blood Cells ◽  
Sampling Rate ◽  
Alpha Fetoprotein ◽  
Optical Cell ◽  
Standard Curve ◽  
Cell Counter

Abstract PACIA, a homogeneous non-radioimmunoassay, has been adapted to the determination of serum alpha 1-fetoprotein. This technique is based on the agglutination of latex particles coated with antibodies to the antigen to be determined. The agglutination is measured by using an optical cell counter designed to count blood cells, to determine the reduction in the number of non-agglutinated particles. Interferences by serum constituents are avoided by coating the particles with the F(ab')2-fragments of th immunoglobulin G fraction of the antiserum. The system is automated, with a sampling rate of 50/h and an incubation time of 26 min. Concentrations used in preparing the standard curve ranged from 1 to 50 microgram/L; analytical recoveries were 93.5 to 98.4%; the correlation coefficient of PACIA with radioimmunoassay, calculated from results on 127 samples, was 0.98; maximum within- and between-assay CVs were 7.4% and 9.6%, respectively.

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