scholarly journals Cervical Cat Scratch Disease Lymphadenitis in a Patient with Immunoglobulin M Antibodies to Toxoplasma gondii

2002 ◽  
Vol 9 (2) ◽  
pp. 496-498
Author(s):  
Mardjan Arvand ◽  
Ilkay Kazak ◽  
Sergije Jovanovic ◽  
Hans-Dieter Foss ◽  
Oliver Liesenfeld
Keyword(s):  
Toxoplasma Gondii ◽  
Clinical Symptoms ◽  
Bartonella Henselae ◽  
Cervical Lymphadenopathy ◽  
Immunoglobulin M ◽  
Cat Scratch Disease ◽  
Specific Immunoglobulin ◽  
Acute Toxoplasmosis

ABSTRACT We report on a young patient with chronic cervical lymphadenopathy and serological and histological evidence for infection with Bartonella henselae and Toxoplasma gondii. Serological follow-up studies, including testing for avidity of Toxoplasma-specific immunoglobulin G antibodies, assisted in the determination of the cause of the acute lymphadenitis. Our results suggest that the clinical symptoms were most likely due to cat scratch disease rather than to acute toxoplasmosis.

scholarly journals Modified Staphylococcal Absorption Method Used for Detecting Rubella-Specific Immunoglobulin M Antibodies During a Rubella Epidemic

1977 ◽  
Vol 5 (6) ◽  
pp. 588-592
Author(s):  
R. Handsher ◽  
A. Fogel
Keyword(s):  
Density Gradient ◽  
Clinical Symptoms ◽  
Density Gradient Centrifugation ◽  
Gradient Centrifugation ◽  
Original Method ◽  
Immunoglobulin M ◽  
Absorption Method ◽  
Igm Antibody ◽  
Specific Immunoglobulin ◽  
History Of

A recently described method for detecting rubella-specific immunoglobulin M (IgM) antibody based on absorption of IgG by Staphylococcus aureus strain Cowan I has been applied to 198 sera collected during a recent rubella epidemic in Israel. Modification of the original method introduced for the present study includes treatment with 2-mercaptoethanol of antibody remaining after absorption by staphylococci. This treatment confirms that the residual antibody is IgM (sensitive to 2-mercaptoethanol) rather than IgG (2-mercaptoethanol resistant). None of the 67 control patients (seropositive for rubella but without history of recent illness or contact) had specific IgM when tested by this method, though 15 showed some residual antibody after staphylococcal absorption. A total of 125 of 131 rubella convalescents (95%) were positive 4 to 49 days after onset of the clinical symptoms. Six patients had no IgM antibodies when tested by the method described, and all were convalescents tested late in relation to onset of clinical symptoms (beyond 3 weeks). When density gradient centrifugation was applied to clarify some results, 2 of 3 convalescents classified as IgM negative by the staphylococcal absorption method did in fact possess IgM antibody. None of 10 controls tested by density gradient centrifugation was IgM positive. This combination of staphylococcal absorption and 2-mercaptoethanol treatment is recommended as a screening test for selection of IgM positives, in addition to the use of a more sensitive method (such as density gradient centrifugation) on at least some samples classified as IgM negative.

scholarly journals Usefulness of Four Different Echinococcus granulosus Recombinant Antigens for Serodiagnosis of Unilocular Hydatid Disease (UHD) and Postsurgical Follow-Up of Patients Treated for UHD

2007 ◽  
Vol 15 (1) ◽  
pp. 147-153 ◽  
Author(s):  
Ana Hernández-González ◽  
Antonio Muro ◽  
Inmaculada Barrera ◽  
Guillermo Ramos ◽  
Antonio Orduña ◽  
...  
Keyword(s):  
Echinococcus Granulosus ◽  
Hydatid Disease ◽  
Characteristic Analysis ◽  
Predictive Values ◽  
Recombinant Antigens ◽  
Hydatid Fluid ◽  
Clinical Laboratories ◽  
Specific Immunoglobulin

ABSTRACT Four different recombinant antigens derived from Echinococcus granulosus, designated B1t, B2t, E14t, and C317, were tested with enzyme-linked immunosorbent assays (ELISAs) for the detection of specific immunoglobulin G (IgG) in patients with unilocular hydatid disease (UHD). The results were compared to those obtained with hydatid fluid and were subjected to receiver operator characteristic analysis. The diagnostic performance of the above-listed proteins was defined with respect to their specificity, sensitivity, and predictive values (PV); the influence of cyst location; and usefulness in the follow-up of surgical treatment for UHD and in the determination of whether or not patients have been surgically cured of UHD. The best diagnostic results were obtained with the anti-B2t IgG ELISA, with 91.2% sensitivity, 93% specificity, and high positive and negative PV (89.4 and 94.2, respectively). In addition, this diagnostic tool proved to be useful for the follow-up of surgically treated UHD patients. The anti-B2t IgG ELISA may find an application in the serodiagnosis of UHD in clinical laboratories.

scholarly journals Development of an Immunoglobulin M Capture-Based Enzyme-Linked Immunosorbent Assay for Diagnosis of Acute Infections with Bartonella henselae

2008 ◽  
Vol 16 (2) ◽  
pp. 282-284 ◽  
Author(s):  
John G. Hoey ◽  
Fernando Valois-Cruz ◽  
Hannah Goldenberg ◽  
Yekaterina Voskoboynik ◽  
Jenna Pfiffner ◽  
...  
Keyword(s):  
Antibody Response ◽  
Immunosorbent Assay ◽  
Bartonella Henselae ◽  
Immunoglobulin M ◽  
Enzyme Linked Immunosorbent Assay ◽  
Cat Scratch Disease ◽  
Specific Enzyme ◽  
Negative Patient ◽  
Bacillary Angiomatosis ◽  
Acute Infections

ABSTRACT We describe the development of an immunoglobulin M-specific enzyme-linked immunosorbent assay for the detection of an early antibody response to Bartonella henselae, the causative agent of cat scratch disease, bacillary angiomatosis, and endocarditis. This assay discriminates between B. henselae-positive and -negative patient samples with sensitivity and specificity values of 100% and 97.1%, respectively.

scholarly journals Disseminated cat-scratch disease: a case report

2015 ◽  
Vol 22 (2) ◽  
pp. 229-231
Author(s):  
Faizul Islam Chowdhury ◽  
MA Kashem ◽  
Md Anwar Hossain ◽  
Imran Munadil Ahsan ◽  
Avijit Pandit ◽  
...  
Keyword(s):  
Bartonella Henselae ◽  
Cervical Lymphadenopathy ◽  
Lymph Node Biopsy ◽  
Cat Scratch Disease ◽  
Gram Negative ◽  
Systemic Involvement ◽  
Medical College ◽  
Negative Bacillus ◽  
Node Biopsy ◽  
Gram Negative Bacillus

Cat-scratch disease is an infectious disease caused by Bartonella henselae, a gram-negative bacillus. The disease is usually transmitted to the human being by cat scratches. Cat-scratch disease typically presents with painful regional lymphadenopathy. Systemic involvement is unusual. A 35 years old lady, came with the complaints of fever, abdominal pain along with nausea, anorexia & difficulties of swallowing for 6 months. She has painful cervical lymphadenopathy. Histopathology of her lymph node biopsy shows features of cat-scratch disease. DOI: http://dx.doi.org/10.3329/jdmc.v22i2.21550 J Dhaka Medical College, Vol. 22, No.2, October, 2013, Page 229-231

scholarly journals Recombinant Antigens To Detect Toxoplasma gondii-Specific Immunoglobulin G and Immunoglobulin M in Human Sera by Enzyme Immunoassay

2000 ◽  
Vol 38 (3) ◽  
pp. 1144-1150 ◽  
Author(s):  
D. Aubert ◽  
G. T. Maine ◽  
I. Villena ◽  
J. C. Hunt ◽  
L. Howard ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Immunoglobulin G ◽  
Acute Infection ◽  
Relative Sensitivity ◽  
Immunoglobulin M ◽  
Recombinant Antigens ◽  
Disease Spectrum ◽  
Human Sera ◽  
Acute Toxoplasmosis ◽  
Sensitivity Specificity

We have evaluated the diagnostic utility of eleven Toxoplasma gondii recombinant antigens (P22 [SAG2], P24 [GRA1], P25, P28 [GRA2], P29 [GRA7], P30 [SAG1], P35, P41 [GRA4], P54 [ROP2], P66 [ROP1], and P68) in immunoglobulin G (IgG) and IgM recombinant enzyme-linked immunosorbent assays (Rec-ELISAs). Following an initial evaluation, six recombinant antigens (P29, P30, P35, P54, P66, and P68) were tested in the IgG and IgM Rec-ELISAs with four groups of samples which span the toxoplasmosis disease spectrum (negative, chronic infection, acute infection, and recent seroconversion). Our results suggest that the combination of P29, P30, and P35 in an IgG Rec-ELISA and the combination of P29, P35, and P66 in an IgM Rec-ELISA can replace the tachyzoite antigen in IgG and IgM serologic tests, respectively. The relative sensitivity, specificity, and agreement for the IgG P29-P30-P35 Rec-ELISA were 98.4, 95.7, and 97.2%, respectively. The resolved sensitivity, specificity, and agreement for the IgM P29-P35-P66 Rec-ELISA were 93.1, 95.0, and 94.5%, respectively. Relative to the tachyzoite-based immunocapture IgM assay, the IgM P29-P35-P66 Rec-ELISA detects fewer samples that contain IgG antibodies with elevated avidity from individuals with an acute toxoplasmosis.

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