scholarly journals Effect of selective PDGF-receptor versus non-selective protein tyrosine kinase blockers on aortic smooth muscle cells (SMC's) and endothelial cells proliferation

1996 ◽  
Vol 27 (2) ◽  
pp. 255 ◽  
Author(s):  
Shmuel Banai ◽  
Aviva Schneider ◽  
S.David Gertz ◽  
Gershon Golomb ◽  
Alexander Levitzki ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Smooth Muscle ◽  
Tyrosine Kinase ◽  
Smooth Muscle Cells ◽  
Protein Tyrosine Kinase ◽  
Muscle Cells ◽  
Pdgf Receptor ◽  
Aortic Smooth Muscle Cells ◽  
Protein Tyrosine ◽  
Aortic Smooth Muscle

scholarly journals Ziziphus nummularia Inhibits Inflammation-Induced Atherogenic Phenotype of Human Aortic Smooth Muscle Cells

2017 ◽  
Vol 2017 ◽  
pp. 1-10 ◽  
Author(s):  
Manal Fardoun ◽  
Tuqa Al-Shehabi ◽  
Ahmed El-Yazbi ◽  
Khodr Issa ◽  
Fouad Zouein ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Muscle Cells ◽  
Matrix Metalloproteases ◽  
Migration And Invasion ◽  
Dependent Manner ◽  
Aortic Smooth Muscle Cells ◽  
Aortic Smooth Muscle ◽  
Ziziphus Nummularia

Cardiovascular disease (CVD) continues to be the leading cause of death worldwide. Atherosclerosis is a CVD characterized by plaque formation resulting from inflammation-induced insults to endothelial cells, monocytes, and vascular smooth muscle cells (VSMCs). Despite significant advances, current treatments for atherosclerosis remain insufficient, prompting the search for alternative modalities, including herbal medicine. Ziziphus nummularia is an herb commonly used in the amelioration of symptoms associated with many health conditions such as cold, diarrhea, cancer, and diabetes. However, its effect on the inflammation-induced behavior of VSMCs remains unknown. In this study, we sought to determine the effect of the ethanolic extract of Z. nummularia (ZNE) on TNF-α-induced phenotypic changes of human aortic smooth muscle cells (HASMCs). The treatment of HASMCs with ZNE decreased cell proliferation, adhesion to fibronectin, migration, and invasion. ZNE treatment also caused a concentration- and time-dependent reduction in the TNF-α-induced expression of matrix metalloproteases MMP-2 and MMP-9, NF-κB, and cell adhesion molecules ICAM-1 and VCAM-1. Furthermore, ZNE decreased the adhesion of THP-1 monocytes to HASMCs and endothelial cells in a concentration-dependent manner. These data provide evidence for the anti-inflammatory effect of Ziziphus nummularia, along with potential implications for its use as an agent that could ameliorate inflammation-induced atherogenic phenotype of VSMCs in atherosclerosis.

H2O2-induced egr-1, fra-1, and c-jun gene expression is mediated by tyrosine kinase in aortic smooth muscle cells

2000 ◽  
Vol 29 (8) ◽  
pp. 736-746 ◽  
Author(s):  
Najia Jin ◽  
Nathan D Hatton ◽  
Maureen A Harrington ◽  
Xiaolin Xia ◽  
Steve H Larsen ◽  
...  
Keyword(s):  
Gene Expression ◽  
Smooth Muscle ◽  
Tyrosine Kinase ◽  
Smooth Muscle Cells ◽  
Muscle Cells ◽  
Aortic Smooth Muscle Cells ◽  
Aortic Smooth Muscle

NO increases protein tyrosine phosphatase activity in smooth muscle cells: relationship to antimitogenesis

1997 ◽  
Vol 272 (3) ◽  
pp. H1342-H1349 ◽  
Author(s):  
G. S. Dhaunsi ◽  
C. Matthews ◽  
K. Kaur ◽  
A. Hassid
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Protein Tyrosine Phosphatase ◽  
Tyrosine Phosphatase ◽  
Muscle Cells ◽  
Aortic Smooth Muscle Cells ◽  
Protein Tyrosine ◽  
Aortic Smooth Muscle ◽  
Cyclic Monophosphate ◽  
Ptpase Activity

We investigated the mechanisms of NO-induced antimitogenesis in primary aortic smooth muscle cells from newborn rats. S-nitroso-N-acetylpenicillamine (SNAP), an NO-releasing agent, decreased basal and growth factor-stimulated DNA synthesis with a threshold effectiveness of 0.3-3 microM. A second NO-releasing agent, 3-morpholinosydnonimine-N-ethylcarbamide, a hydrolysis-resistant cyclic nucleotide, 8-bromo-guanosine 3',5'-cyclic monophosphate (8-BrcGMP), and atrial natriuretic peptides elicited a similar effect, whereas 8-bromo-adenosine 3',5'-cyclic monophosphate (8-BrcAMP) was ineffective, supporting the view that NO and cGMP, but not cAMP, mediated at least some of SNAP's antimitogenic effect. SNAP and 8-BrcGMP decreased the levels of phosphotyrosine, especially in proteins of 70-85 kDa and approximately 215 kDa molecular mass. SNAP decreased protein phosphotyrosine levels with a threshold effectiveness similar to that of its antimitogenic effect. Moreover, SNAP increased protein tyrosine phosphatase (PTPase) activity in cell homogenates, indicating that phosphotyrosine dephosphorylation was likely to be the result of increased PTPase activity. Peroxovanadate, a selective PTPase inhibitor, blocked the antimitogenic effect of 8-BrcGMP, suggesting that loss of protein phosphotyrosine and antimitogenesis were causally linked. These findings describe a potential mechanism for NO-induced antimitogenesis in aortic smooth muscle cells in primary culture.

scholarly journals Human Aortic Smooth Muscle Cells Promote Arteriole Formation by Coengrafted Endothelial Cells

2009 ◽  
Vol 15 (1) ◽  
pp. 165-173 ◽  
Author(s):  
Benjamin R. Shepherd ◽  
Steven M. Jay ◽  
W. Mark Saltzman ◽  
George Tellides ◽  
Jordan S. Pober
Keyword(s):  
Endothelial Cells ◽  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Muscle Cells ◽  
Aortic Smooth Muscle Cells ◽  
Aortic Smooth Muscle
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