Characterization of metabolic changes involved in hypersensitive-like browning reactions of chickpea (Cicer arietinum L.) cell cultures following challenge by Ascochyta rabiei culture filtrate

1994 ◽  
Vol 44 (2) ◽  
pp. 141-155 ◽  
Author(s):  
R. Vogelsang ◽  
E. Berger ◽  
T. Hagedorn ◽  
U. Mühlenbeck ◽  
R. Tenhaken ◽  
...  
Keyword(s):  
Cicer Arietinum ◽  
Cell Cultures ◽  
Culture Filtrate ◽  
Metabolic Changes ◽  
Ascochyta Rabiei ◽  
Cicer Arietinum L ◽  
L Cell

Elicitor-induced metabolic changes in cell cultures of chickpea (Cicer arietinum L.) cultivars resistant and susceptible to Ascochyta rabiei

Planta ◽  
1990 ◽  
Vol 182 (2) ◽  
pp. 270-278 ◽  
Author(s):  
Susanne Daniel ◽  
Karin Tiemann ◽  
Uta Wittkampf ◽  
Wolfang Bless ◽  
Walter Hinderer ◽  
...  
Keyword(s):  
Cicer Arietinum ◽  
Cell Cultures ◽  
Metabolic Changes ◽  
Ascochyta Rabiei ◽  
Cicer Arietinum L

Partial Characterization of an Enzyme from the Fungus Ascochyta rabiei for the Reductive Cleavage of Pterocarpan Phytoalexins to 2′-Hydroxyisoflavans

1987 ◽  
Vol 42 (7-8) ◽  
pp. 897-901 ◽  
Author(s):  
Birgit Höhl ◽  
Wolfgang Barz
Keyword(s):  
Cicer Arietinum ◽  
Crude Protein ◽  
Pathogenic Fungus ◽  
Reductive Cleavage ◽  
Partial Characterization ◽  
Ascochyta Rabiei ◽  
Kinetic Properties ◽  
Ether Bond ◽  
Cicer Arietinum L

Crude protein extracts from the chickpea (Cicer arietinum L.) pathogenic fungus Ascochyta rabiei catalyze the NADPH dependent conversion of the pterocarpan phytoalexins medicarpin and maackiain to 1) 2′-hydroxyisoflavans, 2) 1 a-hydroxypterocarp-1,4-diene-3-one, and 3) 10,2′-dihydroxyisoflav-8-ene-7-one derivatives. A pterocarpan : NADPH oxidoreductase which cleaves the benzylphenyl ether bond of the phytoalexins was purified some 100-fold and partially characterized with regard to its kinetic properties. The oxidoreductase was shown to be specific for NADPH and 3-hydroxypterocarpans. The enzyme appears to be constitutively expressed by A. rabiei.

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