Influence of Age on the Release of Reactive Oxygen Species by Phagocytes as Measured by a Whole Blood Chemiluminescence Assay

1997 ◽  
Vol 22 (3) ◽  
pp. 433-438 ◽  
Author(s):  
Elisabeth M Kukovetz ◽  
Gerhard Bratschitsch ◽  
Herwig P Hofer ◽  
Gerd Egger ◽  
Rudolf J Schaur
Keyword(s):  
Reactive Oxygen Species ◽  
Whole Blood ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Chemiluminescence Assay

scholarly journals Efficacy of α-tocopherol administration in community-acquired pneumonia

2007 ◽  
pp. 99-102
Author(s):  
F. R. Farkhutdinov
Keyword(s):  
Reactive Oxygen Species ◽  
Whole Blood ◽  
Blood Concentration ◽  
Conventional Treatment ◽  
Clinical Course ◽  
Reactive Oxygen ◽  
Redox Status ◽  
Community Acquired Pneumonia ◽  
Oxygen Species ◽  
Laboratory Parameters

We studied effect of α tocopherol on clinical course and production of reactive oxygen species (ROS) in the whole blood in patients with community acquired pneumonia (CAP). The trial involved 70 patients with CAP. Generation of ROS was studied using the luminol dependent chemilumines cence (LDCL) method. Conventional treatment was given to all the patients. Besides this, 35 patients received α tocopherol. LDCL intensity of the blood was enhanced in all the patients. Treatment with α-tocopherol decreased ROS blood concentration and resulted in positive dynamics of clini cal and laboratory parameters. By contrast, patients on the conventional treatment maintained high LDCL intensity and there was slowly resolved course of inflammation in many cases. So, α tocopherol improved redox status in patients with CAP and increased efficiency of the treatment.

Role of histamine receptors in the effects of histamine on the production of reactive oxygen species by whole blood phagocytes

Life Sciences ◽  
2014 ◽  
Vol 100 (1) ◽  
pp. 67-72 ◽  
Author(s):  
Ondrej Vasicek ◽  
Antonin Lojek ◽  
Viera Jancinova ◽  
Radomir Nosal ◽  
Milan Ciz
Keyword(s):  
Reactive Oxygen Species ◽  
Whole Blood ◽  
Reactive Oxygen ◽  
Histamine Receptors ◽  
Oxygen Species

scholarly journals Chemiluminescence assay for quinones based on generation of reactive oxygen species through the redox cycle of quinone

2008 ◽  
Vol 393 (4) ◽  
pp. 1337-1343 ◽  
Author(s):  
Naoya Kishikawa ◽  
Nobuhiro Ohkubo ◽  
Kaname Ohyama ◽  
Kenichiro Nakashima ◽  
Naotaka Kuroda
Keyword(s):  
Reactive Oxygen Species ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Redox Cycle ◽  
Chemiluminescence Assay

Chemiluminescence assay for reactive oxygen species scavenging activities and inhibition on oxidative damage of DNA inDeinococcus radiodurans

Luminescence ◽  
2004 ◽  
Vol 19 (2) ◽  
pp. 78-84 ◽  
Author(s):  
Bing Tian ◽  
Yuanyuan Wu ◽  
Duohong Sheng ◽  
Zhiguo Zheng ◽  
Guanjun Gao ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Oxidative Damage ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Chemiluminescence Assay

scholarly journals The effects of berberine on reactive oxygen species production in human neutrophils and in cell-free assays

2017 ◽  
Vol 10 (2) ◽  
pp. 61-65 ◽  
Author(s):  
Rami B. Kassab ◽  
Ondrej Vasicek ◽  
Milan Ciz ◽  
Antonin Lojek ◽  
Tomas Perecko
Keyword(s):  
Reactive Oxygen Species ◽  
Antioxidant Activity ◽  
Whole Blood ◽  
Reactive Oxygen Species Production ◽  
Reactive Oxygen ◽  
Immune Defense ◽  
Peroxyl Radicals ◽  
Human Neutrophils ◽  
Oxygen Species ◽  
Species Production

AbstractThe health benefits of berberine have been recognized for years. Even so, its effects on human neutrophils, the first line of immune defense, have not been reported. The purpose of this study was to investigate the effects of berberine on the human neutrophil oxidative burst. Reactive oxygen species production was analyzed by luminol-enhanced chemiluminescence. The analysis was performed in spontaneous and stimulated (phorbol myristate acetate (PMA) or opsonized zymosan particles (OZP)) whole blood and isolated neutrophils in the presence or absence of berberine. The effects of berberine on oxidant production in cell-free assays were evaluated using luminescence (H2O2-peroxidase-luminol) and fluorescence (Oxygen Radical Absorbance Capacity – ORAC) techniques. Berberine decreased the production of reactive oxygen species in human whole blood and isolated neutrophils stimulated with either PMA or OZP with a different efficiency (EC50was 69 μM and 197 μM for PMA and OZP, respectively). The effect was more pronounced in isolated neutrophils. Cell-free assays showed the antioxidant activity of berberine against peroxyl radicals and hydrogen peroxide. Based on our results, we suggest that the effects of berberine on reactive oxygen species production in human neutrophils are due to its antioxidant activity.

Critical limb ischaemia is characterised by an increased production of whole blood reactive oxygen species and expression of TREM-1 on neutrophils

2013 ◽  
Vol 229 (2) ◽  
pp. 396-403 ◽  
Author(s):  
Jörn F. Dopheide ◽  
Christopher Doppler ◽  
Martin Scheer ◽  
Viviane Obst ◽  
Marie-Christine Radmacher ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Whole Blood ◽  
Reactive Oxygen ◽  
Critical Limb Ischaemia ◽  
Limb Ischaemia ◽  
Oxygen Species
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