P935 Time-to-positivity of aerobic blood cultures by using BacTec 9120 blood culture system

2007 ◽  
Vol 29 ◽  
pp. S244-S245
Author(s):  
St. Fokas ◽  
Sp. Fokas ◽  
G. Altouvas ◽  
M. Tsironi ◽  
S. Kaptanis ◽  
...  
Keyword(s):  
Blood Culture ◽  
Culture System ◽  
Blood Cultures ◽  
Blood Culture System ◽  
Time To Positivity

scholarly journals Comparative study of manual conventional blood cultures versus automated blood culture system in cases of septicemia

2021 ◽  
Vol 8 (4) ◽  
pp. 327-332
Author(s):  
Humera Qudsia Fatima Ansari ◽  
Lubna Saher ◽  
Mustafa Afzal
Keyword(s):  
Blood Culture ◽  
Culture System ◽  
Blood Cultures ◽  
Diffusion Method ◽  
Suspected Case ◽  
Care Hospital ◽  
Susceptibility Pattern ◽  
Gold Standard Method ◽  
Blood Culture System ◽  
Culture Systems

: Blood cultures are a proven gold standard method for the identification of causative agents of bloodstream infections. Identification of causative organism along with antibiotic susceptibility plays a pivotal role in proposing suitable antibiotic therapy. Automated blood culture systems show improved monitoring of blood cultures by reducing the time and by ensuring more accurate results when compared to the conventional blood culture system. To isolate the organism from given blood samples of a suspected case of septicemia and to compare the results of conventional and automated blood culture systems and to study the antimicrobial susceptibility pattern of the pathogens isolated. A prospective study of 6 months period was conducted among 100 subjects attending the Department of Microbiology in a tertiary care hospital. Subjects with symptoms and signs of septicemia were included. 25ml of venous blood was drawn aseptically from the venipuncture site, of which 5ml of blood was inoculated into 50ml of Brain Heart Infusion bottle in conventional blood culture system and 10ml each into aerobic and anaerobic BACTEC PLUS bottle in Automated blood culture system BACTEC FX40. Overall, 48% and 60% of the samples revealed positive growth by the conventional and automated blood culture system BACTEC FX40, respectively. Gram Positive Cocci were 52.08% and Gram Negative Bacilli were 47.91% isolated by conventional blood culture system, whereas automated blood culture system BACTEC FX40 isolated 45% and 55%, respectively. Isolates were detected within 24-48hrs and 12-24 hrs by conventional and automated blood culture systems, respectively. The anti-microbial susceptibility pattern of the pathogens isolated was also recorded by Kirby Bauer disc diffusion method of antimicrobial susceptiblity testing. Automated blood culture systems are a trustworthy substitute to conventional blood culture systems. The automated blood culture systems being more sensitive and rapid in detecting septicemia in subjects acts as an appropriate means for the initial identification and detection of blood pathogens and improved provision of antimicrobial therapeutic options for septic Patients especially in Critical Care and Intensive Care Units where positive culture reporting is crucial.

Rapid Detection of Microorganisms in Blood Cultures of Newborn Infants Utilizing an Automated Blood Culture System

PEDIATRICS ◽  
2000 ◽  
Vol 105 (3) ◽  
pp. 523-527 ◽  
Author(s):  
Joseph A. Garcia-Prats ◽  
Timothy R. Cooper ◽  
Virginia F. Schneider ◽  
Charles E. Stager ◽  
Thomas N. Hansen
Keyword(s):  
Blood Culture ◽  
Rapid Detection ◽  
Culture System ◽  
Newborn Infants ◽  
Blood Cultures ◽  
Blood Culture System

scholarly journals 292. Impact of the BACT/ALERT VIRTUO blood culture system in the management of Staphylococcus aureus bacteremia

2020 ◽  
Vol 7 (Supplement_1) ◽  
pp. S145-S146
Author(s):  
Miguel A Chavez ◽  
Satish Munigala ◽  
Carey-Ann Burnham ◽  
David K Warren
Keyword(s):  
Staphylococcus Aureus ◽  
Blood Culture ◽  
Median Time ◽  
Culture System ◽  
Source Control ◽  
Similar Proportion ◽  
Blood Culture System ◽  
Staphylococcus Aureus Bacteremia ◽  
Time To Positivity ◽  
Implementation Period

Abstract Background Staphylococcus aureus bacteremia (SAB) is a major cause of mortality. Recovery of SA may be enhanced with new blood culture systems resulting in a longer observed duration of bacteremia. Methods We performed a 24-month retrospective study of adults hospitalized with SAB at a 1250-bed academic hospital. Between 1/2018-12/2018 the VersaTREK system was used and 1/2019-12/2019 the BACT/ALERT VIRTUO (VIRTUO) system was used. We excluded patients without an Infectious Diseases (ID) consult. We defined SAB duration as short (1–2 days), intermediate (3–6 days), or prolonged (>7 days). We compared SAB detection and management pre- and post-implementation of VIRTUO. Results 456 patients had SAB during study period; 420 (92%) had ID consultation: 178 (42%) pre- and 242 (58%) post-implementation. Similar proportion of methicillin-resistant SAB was seen (44.9% pre- vs. 36.8% post-implementation, p=0.09). Post-implementation, patients were more likely to have intermediate (22.4% pre- vs. 40.1% post-implementation; p< 0.001) and prolonged SAB duration (3.9% pre- vs. 13.6% post-implementation; p< 0.001). Median time to positivity for the index blood culture was shorter (19.9 pre- vs. 15.0 hours post-implementation, p< 0.001). Dual anti-staphylococcal therapy was used more frequently in the post-implementation period (6.2% pre- vs. 15.7% post-implementation, p=0.003). No difference was noted in frequency of diagnostic studies (transesophageal echocardiography, magnetic resonance imaging, and computed tomography). Source control was similar (46.1% pre- vs. 45.0% post-implementation; p=0.84) but the median time to source-control was shorter post-implementation (4 pre- vs. 2 days post-implementation; p=0.02). Median planned duration of intravenous antibiotics did not vary between pre- and post-implementation periods (6 vs. 6 weeks, p=0.31). There was no difference in 90-day readmissions (38.2% pre- vs. 34.3% post-implementation; p=0.41). Conclusion VIRTUO blood culture system decreased time to positivity and increased frequency of prolonged SAB compared to the VersaTREK system. This resulted in increased use of dual anti-staphylococcal therapy and shorter time to source-control, but no difference in interventions, planned duration of antibiotics, or readmissions. Disclosures All Authors: No reported disclosures

scholarly journals Evaluation of Four Methods for Rapid Identification of Staphylococcus aureus from Blood Cultures

1998 ◽  
Vol 36 (4) ◽  
pp. 1032-1034 ◽  
Author(s):  
David J. Speers ◽  
Thomas R. Olma ◽  
Gwendolyn L. Gilbert
Keyword(s):  
Staphylococcus Aureus ◽  
Blood Culture ◽  
Culture System ◽  
Rapid Identification ◽  
Culture Broth ◽  
Blood Cultures ◽  
Blood Culture System ◽  
Variable Sensitivity ◽  
Rapid Tests ◽  
Gram Positive Cocci

The identification of Staphylococcus aureus directly from blood cultures is clinically relevant, but it requires a test that is both rapid and reliable. Previously, biochemical, immunological, tube coagulase, and thermostable-endonuclease methods have shown variable sensitivity and specificity. Testing directly from blood culture broth has not been described for the latex kit Staphaurex Plus (Murex Diagnostics Ltd.), and the modified conventional tests have not been used with the newer, continuously monitored blood culture systems. In addition, the commercial RAPIDEC staph kit (bioMerieux Vitek, Inc.) has been used to detect S. aureus directly from the Vital blood culture system (bioMerieux, Marcy l’Etoile, France), but its performance has not been evaluated with other continuously monitored systems. A total of 201 clinical blood cultures (BACTEC 9240 culture system; Johnston Laboratories, Inc.) in which a Gram stain showed gram-positive cocci resembling staphylococci were evaluated prospectively. The Staphaurex Plus kit, the tube coagulase test, the thermostable-endonuclease test, and the RAPIDEC staph kit were compared. The sensitivities were 23, 92, 85, and 98% and the specificities were 99, 100, 93, and 100%, respectively. The RAPIDEC staph kit was the most reliable test, with a diagnostic accuracy comparable to that of the best published results for any of the rapid tests. However, it was the most expensive of the tests and relatively labor-intensive. The tube coagulase test was also sensitive, the simplest to perform, and inexpensive.

scholarly journals Isolation of pathogenic aerobic bacteria from the blood of septicaemic neonatal calves and the susceptibility of isolates to various antibiotics

2010 ◽  
Vol 81 (2) ◽  
Author(s):  
E. Kirecci ◽  
Y. Ozkanlar ◽  
M.S. Aktas ◽  
M.H. Uyanik ◽  
H. Yazgi
Keyword(s):  
Blood Culture ◽  
Culture System ◽  
Blood Cultures ◽  
Aerobic Bacteria ◽  
Blood Culture System ◽  
Amoxicillin Clavulanic Acid ◽  
Bactec System ◽  
Neonatal Calves ◽  
Susceptibility Rate ◽  
Corynebacterium Sp

An automated blood culture system (BACTEC 9240) was used for the isolation of aerobic bacteria from the blood of septicaemic neonatal calves. Blood samples were collected from 90 clinically septicaemic and 20 healthy neonatal calves and inoculated into blood culture bottles. There were 89 significant isolates from 90 positive blood cultures using the BACTEC system. Escherichia coli was the most common pathogen detected accounting for 56 (63 %) out of 89 isolates. The other pathogens were β-haemolytic streptococci (15.7 %), Staphylococcusaureus (10.1 %), Klebsiella sp. (5.6 %) and Corynebacterium sp. (5.6 %). All isolates showed a susceptibility rate of 100 % to enrofloxacin, cefepim, cefoperazone / sulbactam, imipenem and meropenem while some of them were ranged from 75 to 91.7 % susceptible to amoxicillin / clavulanic acid, ampicillin / sulbactam, gentamicin and cephalosporins.

scholarly journals Evaluation of blood culture procedures in a pediatric hospital

1979 ◽  
Vol 9 (1) ◽  
pp. 88-92
Author(s):  
E G Szymczak ◽  
J T Barr ◽  
W A Durbin ◽  
D A Goldmann
Keyword(s):  
Blood Culture ◽  
Culture System ◽  
Clinical Laboratory ◽  
Blood Cultures ◽  
Pediatric Hospital ◽  
Visual Examination ◽  
Clinical Laboratory Techniques ◽  
Blood Culture System ◽  
Laboratory Techniques ◽  
Clinically Significant

To determine optimal clinical laboratory techniques for detecting pediatric bacteremia, we studied 7,768 consecutive blood cultures in a 1-year period. Blood was inoculated into one vented 50-ml bottle of brucella broth with 0.05% sodium polyanetholsulfonate and one unvented 50-ml bottle of Columbia broth with 0.05% sodium polyanetholsulfonate and 0.05% cysteine. Bottles were visually examined for growth on days 1 through 7 and blindly subcultured aerobically and anaerobically on days 1, 2, and 7. There were 724 (9.3%) positive cultures, and 484 (6.2%) were clinically significant. The most frequent isolates from bacteremic patients were Haemophilus influenzae (24%) and Streptococcus pneumoniae (17%). Growth was noted in only one bottle in 25% of clinically significant isolates. Bottles inoculated with greater than or equal to 1 ml of blood became positive earlier than bottles inoculated with less than 1 ml. After 1 day of incubation, 48% of the clinically significant cultures showed growth on visual examination, whereas 85% showed growth on subculture. Only 19% of Haemophilus isolates were detected visually on day 1, whereas 88% were recovered on subculture. By day 7, 3.5% of all isolates (including 18% of pneumococcal isolates and 1% of Haemophilus isolates) could no longer be recovered on subculture. We conclude that a two-bottle blood culture system and blind subculture within 24 h will optimize detection of pediatric bacteremia.

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