scholarly journals Comparative study of manual conventional blood cultures versus automated blood culture system in cases of septicemia

2021 ◽  
Vol 8 (4) ◽  
pp. 327-332
Author(s):  
Humera Qudsia Fatima Ansari ◽  
Lubna Saher ◽  
Mustafa Afzal
Keyword(s):  
Blood Culture ◽  
Culture System ◽  
Blood Cultures ◽  
Diffusion Method ◽  
Suspected Case ◽  
Care Hospital ◽  
Susceptibility Pattern ◽  
Gold Standard Method ◽  
Blood Culture System ◽  
Culture Systems

: Blood cultures are a proven gold standard method for the identification of causative agents of bloodstream infections. Identification of causative organism along with antibiotic susceptibility plays a pivotal role in proposing suitable antibiotic therapy. Automated blood culture systems show improved monitoring of blood cultures by reducing the time and by ensuring more accurate results when compared to the conventional blood culture system. To isolate the organism from given blood samples of a suspected case of septicemia and to compare the results of conventional and automated blood culture systems and to study the antimicrobial susceptibility pattern of the pathogens isolated. A prospective study of 6 months period was conducted among 100 subjects attending the Department of Microbiology in a tertiary care hospital. Subjects with symptoms and signs of septicemia were included. 25ml of venous blood was drawn aseptically from the venipuncture site, of which 5ml of blood was inoculated into 50ml of Brain Heart Infusion bottle in conventional blood culture system and 10ml each into aerobic and anaerobic BACTEC PLUS bottle in Automated blood culture system BACTEC FX40. Overall, 48% and 60% of the samples revealed positive growth by the conventional and automated blood culture system BACTEC FX40, respectively. Gram Positive Cocci were 52.08% and Gram Negative Bacilli were 47.91% isolated by conventional blood culture system, whereas automated blood culture system BACTEC FX40 isolated 45% and 55%, respectively. Isolates were detected within 24-48hrs and 12-24 hrs by conventional and automated blood culture systems, respectively. The anti-microbial susceptibility pattern of the pathogens isolated was also recorded by Kirby Bauer disc diffusion method of antimicrobial susceptiblity testing. Automated blood culture systems are a trustworthy substitute to conventional blood culture systems. The automated blood culture systems being more sensitive and rapid in detecting septicemia in subjects acts as an appropriate means for the initial identification and detection of blood pathogens and improved provision of antimicrobial therapeutic options for septic Patients especially in Critical Care and Intensive Care Units where positive culture reporting is crucial.

scholarly journals Comparative study between automated blood culture and conventional blood culture in neonatal septicaemia cases isolated in a tertiary care hospital in Odisha

2020 ◽  
Vol 8 (12) ◽  
pp. 4267
Author(s):  
Kausik Kumar Sarangi ◽  
Dipti Pattnaik ◽  
Surya Narayan Mishra ◽  
Manas Kumar Nayak ◽  
Jagadananda Jena
Keyword(s):  
Developing Countries ◽  
Blood Culture ◽  
Neonatal Mortality ◽  
Culture System ◽  
Blood Cultures ◽  
Care Hospital ◽  
Essential Information ◽  
Blood Culture System ◽  
Neonatal Septicaemia ◽  
Vitek 2

Background: Neonatal sepsis is the third leading cause of neonatal mortality and a major public health problem, especially in developing countries.  In developing countries sepsis being cause of neonatal mortality is responsible for 30-50% of the 5 million of total neonatal deaths each year. The detection of microorganisms in a patient's blood has a great diagnostic and prognostic significance. Blood cultures provide essential information for the evaluation of a variety of diseases like endocarditis, pneumonia, and pyrexia of unknown origin particularly in patients with suspected sepsis.  In our study we have done blood cultures from patients on a neonatal intensive care unit by both automated and conventional system simultaneously and have done comparative analysis between the two systems.Methods: The aim of this study was to compare the results of blood culture employing the conventional and BacT/Alert and VITEK-2 methods for detection of neonatal septicaemia cases. A prospective study was carried out in the Department of Microbiology in association with Department of Paediatrics and NICU, of Kalinga Institute of Medical Sciences, Bhubaneswar. 250 neonates with clinically suspected septicaemia were included in the study group. Three (3) ml of venous blood was collected aseptically of which 2ml was cultured by automated BacT/Alert and VITEK-2 method for rapid isolation and sensitivity test and rest 1 ml of blood for conventional culture.Results: Isolation of bacterial pathogens by culture using the automated system showed greater positivity (32.8%) as compared to 18% by conventional blood culture system.Conclusions: This study shows that automated blood culture system is superior to conventional blood culture system in terms of rapid and specific isolation of organism.

scholarly journals 650. Clinical Performance Evaluation of Virtuo Blood Culture System in a Tertiary Care Hospital

2020 ◽  
Vol 7 (Supplement_1) ◽  
pp. S383-S383
Author(s):  
Miguel A Chavez ◽  
Satish Munigala ◽  
Carey-Ann Burnham ◽  
Melanie L Yarbrough ◽  
David K Warren
Keyword(s):  
Blood Culture ◽  
Recovery Rate ◽  
Culture System ◽  
Tertiary Care ◽  
Blood Cultures ◽  
Care Hospital ◽  
Blood Culture System ◽  
Culture Positivity ◽  
Blood Culture Positivity ◽  
Staphylococcus Spp

Abstract Background Bloodstream infections are a major cause of morbidity and mortality. BACT/ALERT VIRTUO (VIRTUO) blood culture system is an automated, closed system used with resin-containing media which may enhance the growth of microorganisms. Our objective was to assess the real-world performance of the VIRTUO system. Methods We retrospectively reviewed all blood cultures performed between January-December 2018 (VersaTREK) and January-December 2019 (VIRTUO) at a 1250-bed academic medical center. Blood culture positivity rates, contamination rates, and time from collection to arrival in the laboratory were compared pre- versus post-VIRTUO implementation. Contamination was defined as a single blood culture with common skin microbiota. Results A total of 101803 blood cultures were performed during the study period: 48969 (48.1%) were processed with VersaTREK system and 52834 (51.9%) with VIRTUO system. A decreased median time from collection until arrival to the laboratory was seen post-implementation (2.0 pre- vs. 0.8 hours post-implementation, p< 0.001). The positivity rate increased from 3987 (8.1%) pre-implementation to 6141 (11.6%) post-implementation (p < 0.001) (Table and Figure). Staphylococcus aureus was the most frequently isolated species for both periods and had higher recovery rate with the VIRTUO system (717 (1.5%) pre- vs. 1764 (3.3%) post-implementation, p< 0.001). Higher recovery rate was also noted for other Staphylococcus spp. in the post-implementation period (985 (2.0%) pre- vs. 1644 (3.1%) post-implementation, p< 0.001). No difference in the organism recovery rate was noted for Streptococcus spp., Enterococcus faecium, E. faecalis, Pseudomonas aeruginosa, Enterobacterales, and Candida spp. The inpatient contamination rate was higher post-implementation (1.5% pre- vs. 1.9% post-implementation, p < 0.001). Comparison of blood culture positivity rate pre- vs. post-implementation, by culture location Daily positivity rate for blood cultures processed at BJH during the study period Conclusion The VIRTUO system showed a higher rate of positive blood cultures compared to the VersaTREK system primarily from a higher detection of Staphylococcus spp. Further studies are needed to assess whether an increased rate of positive blood cultures is associated with changes in management and clinical outcomes. Disclosures All Authors: No reported disclosures

Rapid Detection of Microorganisms in Blood Cultures of Newborn Infants Utilizing an Automated Blood Culture System

PEDIATRICS ◽  
2000 ◽  
Vol 105 (3) ◽  
pp. 523-527 ◽  
Author(s):  
Joseph A. Garcia-Prats ◽  
Timothy R. Cooper ◽  
Virginia F. Schneider ◽  
Charles E. Stager ◽  
Thomas N. Hansen
Keyword(s):  
Blood Culture ◽  
Rapid Detection ◽  
Culture System ◽  
Newborn Infants ◽  
Blood Cultures ◽  
Blood Culture System

P935 Time-to-positivity of aerobic blood cultures by using BacTec 9120 blood culture system

2007 ◽  
Vol 29 ◽  
pp. S244-S245
Author(s):  
St. Fokas ◽  
Sp. Fokas ◽  
G. Altouvas ◽  
M. Tsironi ◽  
S. Kaptanis ◽  
...  
Keyword(s):  
Blood Culture ◽  
Culture System ◽  
Blood Cultures ◽  
Blood Culture System ◽  
Time To Positivity

scholarly journals Evaluation of Four Methods for Rapid Identification of Staphylococcus aureus from Blood Cultures

1998 ◽  
Vol 36 (4) ◽  
pp. 1032-1034 ◽  
Author(s):  
David J. Speers ◽  
Thomas R. Olma ◽  
Gwendolyn L. Gilbert
Keyword(s):  
Staphylococcus Aureus ◽  
Blood Culture ◽  
Culture System ◽  
Rapid Identification ◽  
Culture Broth ◽  
Blood Cultures ◽  
Blood Culture System ◽  
Variable Sensitivity ◽  
Rapid Tests ◽  
Gram Positive Cocci

The identification of Staphylococcus aureus directly from blood cultures is clinically relevant, but it requires a test that is both rapid and reliable. Previously, biochemical, immunological, tube coagulase, and thermostable-endonuclease methods have shown variable sensitivity and specificity. Testing directly from blood culture broth has not been described for the latex kit Staphaurex Plus (Murex Diagnostics Ltd.), and the modified conventional tests have not been used with the newer, continuously monitored blood culture systems. In addition, the commercial RAPIDEC staph kit (bioMerieux Vitek, Inc.) has been used to detect S. aureus directly from the Vital blood culture system (bioMerieux, Marcy l’Etoile, France), but its performance has not been evaluated with other continuously monitored systems. A total of 201 clinical blood cultures (BACTEC 9240 culture system; Johnston Laboratories, Inc.) in which a Gram stain showed gram-positive cocci resembling staphylococci were evaluated prospectively. The Staphaurex Plus kit, the tube coagulase test, the thermostable-endonuclease test, and the RAPIDEC staph kit were compared. The sensitivities were 23, 92, 85, and 98% and the specificities were 99, 100, 93, and 100%, respectively. The RAPIDEC staph kit was the most reliable test, with a diagnostic accuracy comparable to that of the best published results for any of the rapid tests. However, it was the most expensive of the tests and relatively labor-intensive. The tube coagulase test was also sensitive, the simplest to perform, and inexpensive.

scholarly journals Isolation of pathogenic aerobic bacteria from the blood of septicaemic neonatal calves and the susceptibility of isolates to various antibiotics

2010 ◽  
Vol 81 (2) ◽  
Author(s):  
E. Kirecci ◽  
Y. Ozkanlar ◽  
M.S. Aktas ◽  
M.H. Uyanik ◽  
H. Yazgi
Keyword(s):  
Blood Culture ◽  
Culture System ◽  
Blood Cultures ◽  
Aerobic Bacteria ◽  
Blood Culture System ◽  
Amoxicillin Clavulanic Acid ◽  
Bactec System ◽  
Neonatal Calves ◽  
Susceptibility Rate ◽  
Corynebacterium Sp

An automated blood culture system (BACTEC 9240) was used for the isolation of aerobic bacteria from the blood of septicaemic neonatal calves. Blood samples were collected from 90 clinically septicaemic and 20 healthy neonatal calves and inoculated into blood culture bottles. There were 89 significant isolates from 90 positive blood cultures using the BACTEC system. Escherichia coli was the most common pathogen detected accounting for 56 (63 %) out of 89 isolates. The other pathogens were β-haemolytic streptococci (15.7 %), Staphylococcusaureus (10.1 %), Klebsiella sp. (5.6 %) and Corynebacterium sp. (5.6 %). All isolates showed a susceptibility rate of 100 % to enrofloxacin, cefepim, cefoperazone / sulbactam, imipenem and meropenem while some of them were ranged from 75 to 91.7 % susceptible to amoxicillin / clavulanic acid, ampicillin / sulbactam, gentamicin and cephalosporins.

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