Immunohistochemical localization of relaxin in the reproductive system of the female opossum (Didelphis virginiana)

AbstractTwo functionally distinct isoforms of warm-temperature acclimation related 65-kDa protein (Wap65-1 and Wap65-2) with a role in the immune response are present in fish. To our knowledge, contrary to Wap65-1, Wap65-2 has neither been isolated nor functionally characterized in carp especially in reproductive system. The aim of this study was to characterize Wap65-2 and ascertain its functions in immune response and temperature acclimation within reproductive system. Wap65-2 corresponded to one of the most abundant proteins in carp seminal plasma, with a high immunologic similarity to their counterparts in seminal plasma of other fish species and a wide tissue distribution, with predominant expression in the liver. The immunohistochemical localization of Wap65-2 to spermatogonia, Leydig cells, and the epithelium of blood vessels within the testis suggests its role in iron metabolism during spermatogenesis and maintenance of blood-testis barrier integrity. Wap65-2 secretion by the epithelial cells of the spermatic duct and its presence around spermatozoa suggests its involvement in the protection of spermatozoa against damage caused by heme released from erythrocytes following hemorrhage and inflammation. Our results revealed an isoform-specific response of Wap65 to temperature acclimation and Aeromonas salmonicida infection which alters blood-testis barrier integrity. Wap65-2 seems to be related to the immune response against bacteria, while Wap65-1 seems to be involved in temperature acclimation. This study expands the understanding of the mechanism of carp testicular immunity against bacterial challenge and temperature changes, in which Wap65-2 seems to be involved and highlights their potential usefulness as biomarkers of inflammation and temperature acclimation.

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Immunohistochemical Localization of Testin in the Female Reproductive System of the Rat is Consistent with its Involvement in the Turnover of Specialized Junctional Complexes1

Biology of Reproduction ◽

10.1095/biolreprod56.5.1330 ◽

1997 ◽

Vol 56 (5) ◽

pp. 1330-1335 ◽

Cited By ~ 9

Author(s):

Li-ji Zhu ◽

A. Moo Moo-Young ◽

C. Wayne Bardin ◽

C. Yan Cheng

Keyword(s):

Reproductive System ◽

Immunohistochemical Localization ◽

Female Reproductive System

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Immunohistochemical Localization of Gastrin-releasing Peptide, Neuronal Nitric Oxide Synthase and Neurone-specific Enolase in the Uterus of the North American Opossum, Didelphis virginiana

Anatomia Histologia Embryologia ◽

10.1111/j.1439-0264.2005.00604.x ◽

2005 ◽

Vol 34 (4) ◽

pp. 225-231

Author(s):

A. Kumano ◽

M. Sasaki ◽

T. Budipitojo ◽

N. Kitamura ◽

W. J. Krause ◽

...

Keyword(s):

Nitric Oxide ◽

Nitric Oxide Synthase ◽

North American ◽

Neuronal Nitric Oxide Synthase ◽

Immunohistochemical Localization ◽

Didelphis Virginiana ◽

Gastrin Releasing Peptide ◽

The North ◽

American Opossum ◽

Oxide Synthase

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Vesicular system associated with spermiogenesis of bullfrog

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100128377 ◽

1987 ◽

Vol 45 ◽

pp. 818-819

Author(s):

K. C. Liu ◽

S. F. Tsay

Keyword(s):

Electron Microscopy ◽

Microscopic Study ◽

Electron Microscopic Study ◽

Reproductive System ◽

Seminiferous Tubule ◽

Rana Catesbeiana ◽

Routine Procedure ◽

Electron Microscopic ◽

Twisted Tubules ◽

Electron Lucent

In the histologic and electron microscopic study of the male reproductive system of bullfrog, Rana catesbeiana, a vesicular system associated with spermiogenesis was observed. It appeared in the lumenal space of the seminiferous tubule (Fig. 1), in the heads of spermatids (Fig. 2), associated with the chromatins of the spermatid (Fig. 4). As deduced from sections, this vesicular system consisted of vesicles of various size or a large group of waving and twisted tubules (Fig. 3), After routine procedure of treatment for electron microscopy, the lumens of both of the vesicles and tubules were electron lucent.In human, vesicles and vesicular system associated with reproductive cell and tissue were reported. In abnormal spermiogenesis, flower-like body, actually vesicles, and giant vesicle associated with the head of spermatid were observed. In both cases the number of vesicle was limited from a single one to a few.

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