Larvicidal activity of Bacillus thuringiensis natural isolates, indigenous to Japan, against two nematoceran insect pests occurring in urban sewage environments

1996 ◽  
Vol 151 (3) ◽  
pp. 263-271 ◽  
Author(s):  
Hiroyuki Saitoh ◽  
Kazuhiko Higuchi ◽  
Eiichi Mizuki ◽  
Michio Ohba
Keyword(s):  
Bacillus Thuringiensis ◽  
Larvicidal Activity ◽  
Insect Pests ◽  
Urban Sewage ◽  
Natural Isolates

SEARCH FOR NATURAL ISOLATES OF Bacillus thuringiensis FOR DEVELOPMENT OF ECOLOGICALLY FRIENDLY BIOLOGICALS

2018 ◽  
Vol 53 (5) ◽  
pp. 1062-1069
Author(s):  
S.D. Grishechkina ◽  
◽  
V.P. Ermolova ◽  
T.A. Romanova ◽  
A.A. Nizhnikov ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
Natural Isolates

A STANDARDIZED BIOASSAY FOR LARVICIDAL ACTIVITY OF BACILLUS THURINGIENSIS IN SUNFLOWER MOTH HOMOEOSOMA ELECTELLUM (LEPIDOPTERA: PHYCITIDAE)

1985 ◽  
Vol 117 (1) ◽  
pp. 15-22 ◽  
Author(s):  
R. G. Lidstone ◽  
D. W. Goerzen ◽  
G. G. Khachatourians
Keyword(s):  
Bacillus Thuringiensis ◽  
Larvicidal Activity ◽  
Standard Test ◽  
Mortality Curve ◽  
Bioassay Technique ◽  
Sunflower Moth ◽  
Homoeosoma Electellum ◽  
Third Instar Larvae

AbstractA standard test for the larvicidal activity of Bacillus thuringiensis (B.t.) against the larvae of the sunflower moth Homoeosoma electellum (Hulst) has been developed. Bioassay parameters investigated include diet preparation, concentration of B.t., effect of formaldehyde, and method of pathogen incorporation in diet. The LC50 for seconded third-instar larvae is 1.24 μg of Dipel® WP ml−1 or 19.8 IU B.t. ml−1 pathogen-incorporated diet. Layering of a pathogen-suspension upon the surface of the diet was not a reliable bioassay technique for H. electellum. The addition of formaldehyde in the diet reduced the slope of the dose–mortality curve but did not change the LC50.

scholarly journals Retargeting of the Bacillus thuringiensis toxin Cyt2Aa against hemipteran insect pests

2013 ◽  
Vol 110 (21) ◽  
pp. 8465-8470 ◽  
Author(s):  
N. P. Chougule ◽  
H. Li ◽  
S. Liu ◽  
L. B. Linz ◽  
K. E. Narva ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
Insect Pests ◽  
Bacillus Thuringiensis Toxin ◽  
Hemipteran Insect

scholarly journals LARVICIDAL ACTIVITY OFTITHONIA DIVERSIFOLIALEAF EXTRACT ONPERI-URBAN MOSQUITO LARVEA INABAKALIKI; ALTERNATIVE TO INSECTICIDE DEVELOPMENT.

2015 ◽  
Vol 7 (1) ◽  
pp. 1225-1228
Author(s):  
UHUO CA ◽  
OKEREKE CN ◽  
NWELE ED ◽  
OGBONNA S.U ◽  
NWANCHOR K.C ◽  
...  
Keyword(s):  
Mortality Rate ◽  
Adverse Effects ◽  
Bioactive Compounds ◽  
Larvicidal Activity ◽  
Urban Areas ◽  
Insect Pests ◽  
Anopheles Mosquito ◽  
Mosquito Population ◽  
Mosquito Larvae ◽  
Dose Dependent

The bioassay activitiesofTithonia diversifolia leave extract was conducted on the larvae of Anopheles mosquito collected at peri-urban areas of Abakaliki Ebonyi State, using the concentrations of the extract in dilutions at 50/100ml, 40/100ml, 30/100ml and 20/100ml introduced with 10 Anopheles mosquito larvae each in four replicates and allowed for 3hrs. Mean mortality rate of the larvae were observed after the first hour, thus 30%, 10%, 05% and 0% respectively while in the 2nd hour were 60%, 40%, 20% and 10% and in the 3rd hour were 80%, 60%, 50% & 30% respectively. The result thus revealed that the treatment is dose dependent and that the studied specie has some bioactive compounds that can be exploited for insect pests control hence observed to be sensitive in anopheles mosquito larvae. Therefore Tithonia diversifolialeaf extract could be used as a bioassay for the control of mosquito due to its active properties as this has exhibited adverse effects on the larvae thereby reducing the mosquito population and thus reducing the malarious infection associated with the bite of mosquito.

Mechanism of action of Bacillus thuringiensis insecticidal toxins and their use in the control of insect pests

2015 ◽  
pp. 858-873 ◽  
Author(s):  
Alejandra Bravo ◽  
Diana L. Martínez de Castro ◽  
Jorge Sánchez ◽  
Pablo Emiliano Cantón ◽  
Gretel Mendoza ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
Mechanism Of Action ◽  
Insect Pests ◽  
Insecticidal Toxins

scholarly journals A Strain of Bacillus thuringiensis Containing a Novel cry7Aa2 Gene that Is Toxic to Leptinotarsa decemlineata (Say) (Coleoptera: Chrysomelidae)

Insects ◽  
2019 ◽  
Vol 10 (9) ◽  
pp. 259 ◽  
Author(s):  
Mikel Domínguez-Arrizabalaga ◽  
Maite Villanueva ◽  
Ana Beatriz Fernandez ◽  
Primitivo Caballero
Keyword(s):  
Amino Acid ◽  
Bacillus Thuringiensis ◽  
Molecular Mass ◽  
Leptinotarsa Decemlineata ◽  
Insect Pests ◽  
Amino Acid Residues ◽  
Cry Protein ◽  
Lc50 Value ◽  
Leptinotarsa Decemlineata Say

The genome of the Bacillus thuringiensis BM311.1 strain was sequenced and assembled in 359 contigs containing a total of 6,390,221 bp. The plasmidic ORF of a putative cry gene from this strain was identified as a potential novel Cry protein of 1138 amino acid residues with a 98% identity compared to Cry7Aa1 and a predicted molecular mass of 129.4 kDa. The primary structure of Cry7Aa2, which had eight conserved blocks and the classical structure of three domains, differed in 28 amino acid residues from that of Cry7Aa1. The cry7Aa2 gene was amplified by PCR and then expressed in the acrystalliferous strain BMB171. SDS-PAGE analysis confirmed the predicted molecular mass for the Cry7Aa2 protein and revealed that after in vitro trypsin incubation, the protein was degraded to a toxin of 62 kDa. However, when treated with digestive fluids from Leptinotarsa decemlineata larvae, one major proteinase-resistant fragment of slightly smaller size was produced. The spore and crystal mixture produced by the wild-type BM311.1 strain against L. decemlineata neonate larvae resulted in a LC50 value of 18.8 μg/mL, which was statistically similar to the estimated LC50 of 20.8 μg/mL for the recombinant BMB17-Cry7Aa2 strain. In addition, when this novel toxin was activated in vitro with commercial trypsin, the LC50 value was reduced 3.8-fold to LC50 = 4.9 μg/mL. The potential advantages of Cry7Aa2 protoxin compared to Cry7Aa1 protoxin when used in the control of insect pests are discussed.

scholarly journals Combinational Effect of Rumex tingitanus (Polygonaceae) Hexane Extract and Bacillus thuringiensis  δ-Endotoxin against Spodoptera littoralis (Lepidoptera: Noctuidae)

2018 ◽  
Vol 2018 ◽  
pp. 1-7 ◽  
Author(s):  
Dhekra Mhalla ◽  
Dalel Ben Farhat-Touzri ◽  
Slim Tounsi ◽  
Mohamed Trigui
Keyword(s):  
Biological Activity ◽  
Chemical Composition ◽  
Bacillus Thuringiensis ◽  
Synergistic Effect ◽  
Spodoptera Littoralis ◽  
Insect Pests ◽  
Hexane Extract ◽  
Active Fraction ◽  
Lepidoptera Noctuidae ◽  
Potential Use

The increasing insect resistance against Bacillus thuringiensis delta-endotoxins is a serious problem which makes it urgent to look for new eco-friendly strategies. Combining these toxins with other biomolecules is one of the promising strategies against insect pests. In this work, we evaluated the bioinsecticidal potential of Rumex tingitanus extracts and B. thuringiensis strain BLB250 against Spodoptera littoralis (Lepidoptera: Noctuidae) larvae. The chemical composition of the hexane extract, the most active fraction, was analyzed to validate the correlation between chemical composition and biological activity. Among the tested extracts, only the hexanic extract showed toxicity against first and second instar larvae with LC50 of 2.56 and 2.95 mg g−1, respectively. The Bacillus thuringiensis BLB250 delta-endotoxins showed toxicity with an LC50 of 56.3 μg g−1. Therefore, the investigated combinational effect of BLB250 delta-endotoxins and R. tingitanus hexane extract proved significant synergistic effect against S. littoralis larvae. The GC-MS analysis of R. tingitanus hexane extract showed the richness of this extract in phytosterols such as β and γ-sitosterol (48.91%), campesterol (6.43%), and β-amyrin (8.92%) which are known for their insecticidal activity. This novel finding highlights the potential use of this combination against insect pests to prevent the appearance of resistance problems.

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