3313 The next generation sequencing (NGS) analysis of selected genes and pathways in clinical stage III cutaneous melanoma

AbstractRecently it has become possible to query the great diversity of natural antibody repertoires using Next Generation Sequencing (NGS). These methods are capable of producing millions of sequences in a single experiment. Here we compare Clinical Stage Therapeutic antibodies to the ∼1b sequences from 60 independent sequencing studies in the Observed Antibody Space Database. Of the 242 post Phase I antibodies, we find 16 with sequence identity matches of 95% or better for both heavy and light chains. There are also 54 perfect matches to therapeutic CDR-H3 regions in the NGS outputs, suggesting a nontrivial amount of convergence between naturally observed sequences and those developed artificially. This has potential implications for both the discovery of antibody therapeutics and the legal protection of commercial antibodies.

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841 Clinical experience with a next-generation sequencing (NGS) test for BRAF and NRAS mutations along with gene expression profiling (GEP) in cutaneous melanoma (CM)

Journal of Investigative Dermatology ◽

10.1016/j.jid.2019.03.917 ◽

2019 ◽

Vol 139 (5) ◽

pp. S145

Author(s):

K.R. Covington ◽

S.J. Kurley ◽

L.E. Meldi-Sholl ◽

D.A. Vargas ◽

T. Poteet ◽

...

Keyword(s):

Gene Expression ◽

Next Generation Sequencing ◽

Gene Expression Profiling ◽

Clinical Experience ◽

Cutaneous Melanoma ◽

Expression Profiling ◽

Next Generation ◽

Next Generation Sequencing Ngs ◽

Generation Sequencing

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Identification of new familial pheochromocytoma/paraganglioma genes using next generation sequencing (NGS)

Endocrine Abstracts ◽

10.1530/endoabs.40.l12 ◽

2016 ◽

Author(s):

Alberto Cascon

Keyword(s):

Next Generation Sequencing ◽

Next Generation ◽

Familial Pheochromocytoma ◽

Next Generation Sequencing Ngs ◽

Generation Sequencing

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Difference in plasma miRNA levels in inferior petrosal sinus samples from patients with ACTH-dependent Cushing’s syndrome as assessed by next-generation sequencing (NGS).

Endocrine Abstracts ◽

10.1530/endoabs.70.aep569 ◽

2020 ◽

Author(s):

Zhanna Belaya ◽

Anastasia Malygina ◽

Alexey Nikitin ◽

Philipp Koshkin ◽

Ivan Sitkin ◽

...

Keyword(s):

Next Generation Sequencing ◽

Cushing’S Syndrome ◽

Cushing's Syndrome ◽

Inferior Petrosal Sinus ◽

Next Generation ◽

Next Generation Sequencing Ngs ◽

Plasma Mirna ◽

Generation Sequencing

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Comprehensive comparison of trophectoderm (TE) and inner cell mass (ICM) by next generation sequencing (NGS)

10.26226/morressier.5af300b2738ab10027aa98b3 ◽

2018 ◽

Author(s):

Ibrahim Elkhatib ◽

Ibrahim ['Alberto']

Keyword(s):

Next Generation Sequencing ◽

Inner Cell Mass ◽

Cell Mass ◽

Next Generation ◽

Comprehensive Comparison ◽

Next Generation Sequencing Ngs ◽

Generation Sequencing

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Next-generation sequencing (NGS) based preimplantation genetic testing for products of reciprocal translocation (PGT-TR) improves diagnosis of the patients by verification of their classical karyotyping results.

10.26226/morressier.5af300b2738ab10027aa98a4 ◽

2018 ◽

Author(s):

Sebastian Pukszta

Keyword(s):

Genetic Testing ◽

Next Generation Sequencing ◽

Reciprocal Translocation ◽

Next Generation ◽

Preimplantation Genetic Testing ◽

Next Generation Sequencing Ngs ◽

Generation Sequencing

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Initial Next-Generation Sequencing (NGS) Results of Alport Syndrome

Journal of Basic and Clinical Health Sciences ◽

10.30621/jbachs.2019.719 ◽

2019 ◽

Author(s):

Altuğ Koç ◽

Elçin Bora ◽

Tayfun Cinleti ◽

Gizem Yıldız ◽

Meral Torun Bayram ◽

...

Keyword(s):

Next Generation Sequencing ◽

Alport Syndrome ◽

Next Generation ◽

Next Generation Sequencing Ngs ◽

Generation Sequencing

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Next Generation Sequencing（NGS）Monitors Minimal Residual Disease（MRD）in Allo-PBSCT Patients

Case Medical Research ◽

10.31525/ct1-nct04126967 ◽

2019 ◽

Author(s):

Keyword(s):

Next Generation Sequencing ◽

Minimal Residual Disease ◽

Residual Disease ◽

Next Generation ◽

Next Generation Sequencing Ngs ◽

Generation Sequencing ◽

Minimal Residual

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The applications of next-generation sequencing (NGS) in drug development for cancer therapy

Current Analytical Chemistry ◽

10.2174/1573411016999200626193227 ◽

2020 ◽

Vol 16 ◽

Author(s):

Pelin Telkoparan-Akillilar ◽

Dilek Cevik

Keyword(s):

Next Generation Sequencing ◽

Drug Discovery ◽

Cancer Therapy ◽

Target Identification ◽

Rapid Development ◽

Next Generation ◽

Biomedical Sciences ◽

Dna And Rna ◽

Next Generation Sequencing Ngs ◽

Generation Sequencing

Background: Numerous sequencing techniques have been progressed since the 1960s with the rapid development of molecular biology studies focusing on DNA and RNA. Methods: a great number of articles, book chapters, websites are reviewed, and the studies covering NGS history, technology and applications to cancer therapy are included in the present article. Results: High throughput next-generation sequencing (NGS) technologies offer many advantages over classical Sanger sequencing with decreasing cost per base and increasing sequencing efficiency. NGS technologies are combined with bioinformatics software to sequence genomes to be used in diagnostics, transcriptomics, epidemiologic and clinical trials in biomedical sciences. The NGS technology has also been successfully used in drug discovery for the treatment of different cancer types. Conclusion: This review focuses on current and potential applications of NGS in various stages of drug discovery process, from target identification through to personalized medicine.

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