Application of a statistical technique to the production of ethanol from sugar beet molasses by Saccharomyces cerevisiae

2000 ◽  
Vol 73 (3) ◽  
pp. 251-255 ◽  
Author(s):  
M Ergun
2010 ◽  
Vol 16 (2) ◽  
pp. 199-206 ◽  
Author(s):  
Hoda Shafaghat ◽  
Ghasem Najafpour ◽  
Sirous Rezaei ◽  
Mazyar Sharifzadeh

Saccharomyces cerevisiae (PTCC 24860) growth on pretreated sugar beet molasses was optimized via statistical approach. In order to liberate all monomeric sugars, pretreated sugar beet molasses with dilute acid was obtained. The influence of process parameters such as sugar concentration, nitrogen source, pH and incubation time on cell growth were investigated by design expert software with application of central composite design (CCD) under response surface methodology (RSM). The optimal culture conditions were pH of 5.3, incubation time of 24 h and medium composition of 35 g reduced sugars, 1.5 g NH4Cl and 1 g yeast extract per liter of the media. At optimal cell growth conditions and incubation time of 12 h, maximum ethanol production of 14.87 g/L was obtained.


2019 ◽  
Vol 8 (1) ◽  
pp. 622-628 ◽  
Author(s):  
Sara Faramarzi ◽  
Younes Anzabi ◽  
Hoda Jafarizadeh-Malmiri

Abstract A bench scale submerged fermentation process was used to bioethanol produce using sugar beet molasses and Saccharomyces cerevisiae, as substrate and microbial strain, respectively. Effects of selenium amount on growth of S. cerevisiae and bioethanol production were evaluated. The obtained results indicated that growth of S. cerevisiae (manifested as turbidity intensity) in the samples containing 0, 5, 10, 15, 20 and 25 μg sodium selenite, during aerobic process, was 0.1707, 0.1678, 0.1679, 0.1664, 0.1627 and 0.160% a.u./h (after 14 h incubation), respectively. Statistical analysis based on compression test indicated that there were insignificant (p > 0.05) differences between growth rate of the yeast in the fermented samples containing S. cerevisiae and 5 to 25 μg selenium salt. Response surface methodology was utilized to evaluate effects of two fermentation parameters namely, amount of selenium (5-25 μg) and substrate brix (10-25°Bx) on the concentration (g/L) of produced bioethanol. Obtained results revealed that maximum bioethanol concentration (55 g/L) was achieved using 15 μg selenium and molasses with 25°Bx. Furthermore, results have also indicated that, without using selenium and using molasses with 25°Bx, bioethanol with concentration of 29 g/L was produced.


2000 ◽  
Vol 882 (1-2) ◽  
pp. 329-334 ◽  
Author(s):  
Roland Ulber ◽  
Robert Faurie ◽  
Peter Sosnitza ◽  
Lutz Fischer ◽  
Egbert Stärk ◽  
...  

2018 ◽  
Vol 125 (1) ◽  
pp. 134-142 ◽  
Author(s):  
Vesna M. Vučurović ◽  
Vladimir S. Puškaš ◽  
Uroš D. Miljić

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