Bioethanol production from sugar beet molasses and thick juice using Saccharomyces cerevisiae immobilized on maize stem ground tissue

Abstract A bench scale submerged fermentation process was used to bioethanol produce using sugar beet molasses and Saccharomyces cerevisiae, as substrate and microbial strain, respectively. Effects of selenium amount on growth of S. cerevisiae and bioethanol production were evaluated. The obtained results indicated that growth of S. cerevisiae (manifested as turbidity intensity) in the samples containing 0, 5, 10, 15, 20 and 25 μg sodium selenite, during aerobic process, was 0.1707, 0.1678, 0.1679, 0.1664, 0.1627 and 0.160% a.u./h (after 14 h incubation), respectively. Statistical analysis based on compression test indicated that there were insignificant (p > 0.05) differences between growth rate of the yeast in the fermented samples containing S. cerevisiae and 5 to 25 μg selenium salt. Response surface methodology was utilized to evaluate effects of two fermentation parameters namely, amount of selenium (5-25 μg) and substrate brix (10-25°Bx) on the concentration (g/L) of produced bioethanol. Obtained results revealed that maximum bioethanol concentration (55 g/L) was achieved using 15 μg selenium and molasses with 25°Bx. Furthermore, results have also indicated that, without using selenium and using molasses with 25°Bx, bioethanol with concentration of 29 g/L was produced.

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Bioethanol production from sugar beet thick juice by Saccharomyces cerevisiae immobilized in alginate-maize stem ground tissue beads

Journal on Processing and Energy in Agriculture ◽

10.5937/jpea1904167v ◽

2019 ◽

Vol 23 (4) ◽

pp. 167-169

Author(s):

Vesna Vučurović ◽

Vladimir Puškaš ◽

Uroš Miljić ◽

Jovana Đuran ◽

Jelena Filipović

Keyword(s):

Saccharomyces Cerevisiae ◽

Sugar Beet ◽

Bioethanol Production ◽

Ground Tissue ◽

Thick Juice

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The effect of changes in opportunity costs and prices of some agricultural products such as sugar beet, corn,wheat etc. used for bioethanol production on the cost of bioethanol production is not adequately known in Turkey.. Therefore, it was aimed to determine the bioethanol production cost items and unit production costs and also to put forth the effects of variations in raw material prices on cost of bioethanol.. The research data were collected via personal interviews from active bioethanol plants in Turkey. The results of previous studies and documents of related institutions and organizations were also used. The study followed classical cost analysis approach to calculate production cost. Scenario analysis was performed when exploring the effect of raw material prices on bioethanol production cost. Research findings showed that production cost per litre bioethanol produced from sugar beet molasses, corn, wheat and corn-wheat mixture were 2.50 TL, 2.84 TL, 2.95 and 2.84 TL, respectively. The share of raw material expenses in bioethanol cost per liter varied associated with the crops used in the process, it was 28.55% for bioethanol produced from sugar beet molasses, 44.81% for bioethanol produced from corn-wheat mixture and 44.87% bioethanol produced from corn. The research results also showed that the changes that occur in raw material prices significantly affected the bioethanol production cost and opportunity cost of crops created difficulties in biomass supply. Implementing the suitable policies and strategies and making the necessary arrangements in legislation would enhance the economic sustainability of bioethanol production in Turkey.

ANADOLU JOURNAL OF AGRICULTURAL SCIENCES ◽

10.7161/omuanajas.289048 ◽

2017 ◽

Vol 32 (1) ◽

pp. 16-16

Author(s):

Selime Canan ◽

Vedat Ceyhan

Keyword(s):

Sugar Beet ◽

Production Cost ◽

Agricultural Products ◽

Production Costs ◽

Raw Material ◽

Bioethanol Production ◽

Beet Molasses ◽

Sugar Beet Molasses ◽

Research Findings ◽

The Cost

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Production of Fructose and Ethanol from Sugar Beet Molasses Using Saccharomyces cerevisiae ATCC 36858

Biotechnology Progress ◽

10.1021/bp010164z ◽

2002 ◽

Vol 18 (2) ◽

pp. 234-239 ◽

Cited By ~ 14

Author(s):

H. Atiyeh ◽

Z. Duvnjak

Keyword(s):

Saccharomyces Cerevisiae ◽

Sugar Beet ◽

Beet Molasses ◽

Sugar Beet Molasses

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Optimal growth of Saccharomyces cerevisiae (PTCC 24860) on pretreated molasses for ethanol production: Application of response surface methodology

Chemical Industry and Chemical Engineering Quarterly ◽

10.2298/ciceq100201029s ◽

2010 ◽

Vol 16 (2) ◽

pp. 199-206 ◽

Cited By ~ 17

Author(s):

Hoda Shafaghat ◽

Ghasem Najafpour ◽

Sirous Rezaei ◽

Mazyar Sharifzadeh

Keyword(s):

Saccharomyces Cerevisiae ◽

Response Surface Methodology ◽

Cell Growth ◽

Sugar Beet ◽

Ethanol Production ◽

Response Surface ◽

Incubation Time ◽

Influence Of Process Parameters ◽

Beet Molasses ◽

Sugar Beet Molasses

Saccharomyces cerevisiae (PTCC 24860) growth on pretreated sugar beet molasses was optimized via statistical approach. In order to liberate all monomeric sugars, pretreated sugar beet molasses with dilute acid was obtained. The influence of process parameters such as sugar concentration, nitrogen source, pH and incubation time on cell growth were investigated by design expert software with application of central composite design (CCD) under response surface methodology (RSM). The optimal culture conditions were pH of 5.3, incubation time of 24 h and medium composition of 35 g reduced sugars, 1.5 g NH4Cl and 1 g yeast extract per liter of the media. At optimal cell growth conditions and incubation time of 12 h, maximum ethanol production of 14.87 g/L was obtained.

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