Reprint of “Cell-free production of G protein-coupled receptors for functional and structural studies” [J. Struct. Biol. 158 (2007) 482–493]☆

2007 ◽  
Vol 159 (2) ◽  
pp. 194-205 ◽  
Author(s):  
Christian Klammt ◽  
Daniel Schwarz ◽  
Nora Eifler ◽  
Andreas Engel ◽  
Jacob Piehler ◽  
...  
Keyword(s):  
G Protein ◽  
G Protein Coupled Receptors ◽  
Structural Studies ◽  
G Protein Coupled

scholarly journals Serial Femtosecond Crystallography of G Protein–Coupled Receptors

2018 ◽  
Vol 47 (1) ◽  
pp. 377-397 ◽  
Author(s):  
Benjamin Stauch ◽  
Vadim Cherezov
Keyword(s):  
G Protein ◽  
G Protein Coupled Receptors ◽  
Structural Studies ◽  
Body Structure ◽  
X Ray ◽  
Physiological Processes ◽  
Recent Emergence ◽  
Mediate Cell ◽  
G Protein Coupled ◽  
Serial Femtosecond Crystallography

G protein–coupled receptors (GPCRs) represent a large superfamily of membrane proteins that mediate cell signaling and regulate a variety of physiological processes in the human body. Structure-function studies of this superfamily were enabled a decade ago by multiple breakthroughs in technology that included receptor stabilization, crystallization in a membrane environment, and microcrystallography. The recent emergence of X-ray free-electron lasers (XFELs) has further accelerated structural studies of GPCRs and other challenging proteins by overcoming radiation damage and providing access to high-resolution structures and dynamics using micrometer-sized crystals. Here, we summarize key technology advancements and major milestones of GPCR research using XFELs and provide a brief outlook on future developments in the field.

Expression of G-protein coupled receptors in Escherichia coli for structural studies

2010 ◽  
Vol 75 (7) ◽  
pp. 881-891 ◽  
Author(s):  
L. E. Petrovskaya ◽  
A. A. Shulga ◽  
O. V. Bocharova ◽  
Ya. S. Ermolyuk ◽  
E. A. Kryukova ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
G Protein ◽  
G Protein Coupled Receptors ◽  
Structural Studies ◽  
G Protein Coupled

Structural Studies on Large Fragments of G Protein Coupled Receptors

2009 ◽  
pp. 309-310
Author(s):  
Fred Naider ◽  
Alexey Neumoin ◽  
Boris Arshava ◽  
Leah S. Cohen ◽  
Jeffrey M. Becker ◽  
...  
Keyword(s):  
G Protein ◽  
G Protein Coupled Receptors ◽  
Structural Studies ◽  
G Protein Coupled

scholarly journals Enhancing functional production of G protein-coupled receptors inPichia pastoristo levels required for structural studies via a single expression screen

2006 ◽  
Vol 15 (5) ◽  
pp. 1115-1126 ◽  
Author(s):  
Nicolas André ◽  
Nadia Cherouati ◽  
Cécile Prual ◽  
Tania Steffan ◽  
Gabrielle Zeder-Lutz ◽  
...  
Keyword(s):  
G Protein ◽  
G Protein Coupled Receptors ◽  
Structural Studies ◽  
G Protein Coupled
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