scholarly journals Large-scale production and protein engineering of G protein-coupled receptors for structural studies

2015 ◽  
Vol 6 ◽  
Author(s):  
Dalibor Milić ◽  
Dmitry B. Veprintsev
Keyword(s):  
Protein Engineering ◽  
G Protein ◽  
Large Scale ◽  
G Protein Coupled Receptors ◽  
Scale Production ◽  
Structural Studies ◽  
Large Scale Production ◽  
G Protein Coupled

scholarly journals Large-scale production and study of a synthetic G protein-coupled receptor: Human olfactory receptor 17-4

2009 ◽  
Vol 106 (29) ◽  
pp. 11925-11930 ◽  
Author(s):  
B. L. Cook ◽  
D. Steuerwald ◽  
L. Kaiser ◽  
J. Graveland-Bikker ◽  
M. Vanberghem ◽  
...  
Keyword(s):  
G Protein ◽  
Olfactory Receptor ◽  
Large Scale ◽  
Scale Production ◽  
G Protein Coupled Receptor ◽  
Large Scale Production ◽  
G Protein Coupled

Mammalian G-protein-coupled receptor expression in Escherichia coli: I. High-throughput large-scale production as inclusion bodies

2009 ◽  
Vol 386 (2) ◽  
pp. 147-155 ◽  
Author(s):  
Kerstin Michalke ◽  
Marie-Eve Gravière ◽  
Céline Huyghe ◽  
Renaud Vincentelli ◽  
Renaud Wagner ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
G Protein ◽  
High Throughput ◽  
Inclusion Bodies ◽  
Large Scale ◽  
Receptor Expression ◽  
Scale Production ◽  
Expression In Escherichia Coli ◽  
Large Scale Production ◽  
G Protein Coupled

Reprint of “Cell-free production of G protein-coupled receptors for functional and structural studies” [J. Struct. Biol. 158 (2007) 482–493]☆

2007 ◽  
Vol 159 (2) ◽  
pp. 194-205 ◽  
Author(s):  
Christian Klammt ◽  
Daniel Schwarz ◽  
Nora Eifler ◽  
Andreas Engel ◽  
Jacob Piehler ◽  
...  
Keyword(s):  
G Protein ◽  
G Protein Coupled Receptors ◽  
Structural Studies ◽  
G Protein Coupled

scholarly journals Enhancement of Characteristics and Potential Applications of Amylases: A Brief Review

2020 ◽  
pp. 24-35 ◽  
Keyword(s):  
Protein Engineering ◽  
Waste Management ◽  
Physicochemical Properties ◽  
Large Scale ◽  
Scale Production ◽  
Plant Products ◽  
Large Scale Production ◽  
Storage Carbohydrate ◽  
High Fructose ◽  
Potential Applications

Starch is the major storage carbohydrate of plant products. Amylases are the group of enzymes hydrolyzes starch and related polymers to smaller oligosaccharides and less amount of monosaccharide. Microbes are the major sources of amylases, exploited for large scale production in different industries. Recently, protein engineering has been applied to improve the structural and physicochemical properties of the enzyme for its potential applications. Amylases are mostly used for liquefaction of starch in the purpose of glucose, maltose, and high fructose containing syrup preparation, malto-oligosaccharides production, desizing, production of bio-fuel, detergent preparation, waste management, and preparation of digestive aids.

scholarly journals Serial Femtosecond Crystallography of G Protein–Coupled Receptors

2018 ◽  
Vol 47 (1) ◽  
pp. 377-397 ◽  
Author(s):  
Benjamin Stauch ◽  
Vadim Cherezov
Keyword(s):  
G Protein ◽  
G Protein Coupled Receptors ◽  
Structural Studies ◽  
Body Structure ◽  
X Ray ◽  
Physiological Processes ◽  
Recent Emergence ◽  
Mediate Cell ◽  
G Protein Coupled ◽  
Serial Femtosecond Crystallography

G protein–coupled receptors (GPCRs) represent a large superfamily of membrane proteins that mediate cell signaling and regulate a variety of physiological processes in the human body. Structure-function studies of this superfamily were enabled a decade ago by multiple breakthroughs in technology that included receptor stabilization, crystallization in a membrane environment, and microcrystallography. The recent emergence of X-ray free-electron lasers (XFELs) has further accelerated structural studies of GPCRs and other challenging proteins by overcoming radiation damage and providing access to high-resolution structures and dynamics using micrometer-sized crystals. Here, we summarize key technology advancements and major milestones of GPCR research using XFELs and provide a brief outlook on future developments in the field.

Sign in / Sign up

Export Citation Format

Share Document