Effect of controlling lactate concentration and periodic change in DO concentration on fermentation characteristics of a mixed culture of Lactobacillus delbrueckii and Ralstonia eutropha for PHB production

2000 ◽  
Vol 89 (4) ◽  
pp. 323-328 ◽  
Author(s):  
Masayuki Tohyama ◽  
Seiji Takagi ◽  
Kazuyuki Shimizu
Keyword(s):  
Mixed Culture ◽  
Ralstonia Eutropha ◽  
Lactate Concentration ◽  
Lactobacillus Delbrueckii ◽  
Periodic Change ◽  
Phb Production ◽  
Do Concentration

Nitrogen removal in a fluidized bed bioreactor by using mixed culture under oxygen-limited conditions

2004 ◽  
Vol 50 (6) ◽  
pp. 313-320 ◽  
Author(s):  
T. Khin ◽  
A.P. Annachhatre
Keyword(s):  
Fluidized Bed ◽  
Mixed Culture ◽  
Nitrogen Removal ◽  
Removal Rate ◽  
Flow Rates ◽  
Fluidized Bed Bioreactor ◽  
Oxygen Gas ◽  
Do Concentration ◽  
Total Nitrogen Removal ◽  
Nitrification And Denitrification

Nitrogen removal involving nitrification and denitrification was investigated in a fluidized bed bioreactor by using mixed culture sludge under oxygen-limited conditions. Methane was used as a sole carbon source for denitrification. In this study, optimal nitrification and denitrification rates were examined by varying methane and oxygen gas dissolution flow rates, 90 ml/min, 400 ml/min and 650 ml/min, in each. Simultaneously nitrification and denitrification was achieved. The total nitrogen removal rate was 15-mg N/g VSS. d, 21-mg N/g VSS. d and 26.4-mg N/g VSS. d at gas dissolution flow rate 90 ml/min, 400 ml/min and 650 ml/min, respectively. No significant accumulation of nitrite was found in this experiment. Nitrogen removal rates depend on gas dissolution flow rates. DO concentration was at 0.5Ð2 mg/L.

Enrichment of Methylocystis dominant mixed culture from rice field for PHB production

2021 ◽  
Author(s):  
Pranav P. Kulkarni ◽  
Sambhaji B. Chavan ◽  
Mandar S. Deshpande ◽  
Dhanishta Sagotra ◽  
Pramod S. Kumbhar ◽  
...  
Keyword(s):  
Mixed Culture ◽  
Rice Field ◽  
Phb Production

Production of poly-3-hydroxybutyrate from mixed culture

Biologia ◽  
2016 ◽  
Vol 71 (7) ◽  
Author(s):  
Thomas Shalin ◽  
Raveendran Sindhu ◽  
Ashok Pandey ◽  
Vincenza Faraco ◽  
Parameswaran Binod
Keyword(s):  
Mixed Culture ◽  
Biodegradable Polymers ◽  
Biodegradable Polymer ◽  
The Other ◽  
Cost Difference ◽  
Microbial Cultures ◽  
Phb Production ◽  
Mixed Microbial Cultures ◽  
Main Barrier ◽  
The Cost

AbstractPoly-3-hydroxybutyrate (PHB) is a biodegradable polymer produced by many bacteria. Some of the properties are similar to thermoplastics like polypropylene, hence finding application PHB can directly replace non-biodegradable polymers. But the main barrier has been the cost difference. The utilization of mixed microbial cultures facilitates the use of complex substrates and thus can reduce the cost of PHB production. In the present study, mixed culture systems, where metabolite produced by one organism may be assimilated by the other organism, were employed.

Growth of a Methane-Utilizing Mixed Culture HD6T on Methanol and Poly-β-Hydroxybutyrate Biosynthesis

2010 ◽  
Vol 160-162 ◽  
pp. 171-175 ◽  
Author(s):  
Jing Dong ◽  
Jia Ying Xin ◽  
Ying Xin Zhang ◽  
Lin Lin Chen ◽  
Hong Ye Liang ◽  
...  
Keyword(s):  
Mixed Culture ◽  
Mineral Salt Medium ◽  
Biomass Concentration ◽  
Dry Weight ◽  
Maximum Growth ◽  
Maximum Growth Rate ◽  
Production Costs ◽  
Lag Phase ◽  
Phase Duration ◽  
Phb Production

Methane-utilizing mixed culture HD6T was successfully cultivated in a brief non-sterile process using methanol as a sole carbon and energy source for the production of poly-β-hydroxybutyrate(PHB). Shake-flask experiments showed HD6T could grow well in the mineral salt medium with the addition of methanol exposed to the air directly. This non-sterile process and the use of cheap substrates (methanol) can reduce the production costs of PHB. It was found that HD6T grew better and PHB production in a more effective way with an initial liquid methanol concentration of 0.15%(v/v).The lag phase duration, the maximum growth rate, the biomass concentration and the PHB yield, for the optimal conditions were, respectively, 12.03h, 0.04h-1(OD600), 1.54g/l(dry weight), 0.424g/l(dry weight). Methane-utilizing mixed culture HD6T appears to be a promising organism for PHB production.

Sign in / Sign up

Export Citation Format

Share Document