Enrichment of Methylocystis dominant mixed culture from rice field for PHB production

2021 ◽  
Author(s):  
Pranav P. Kulkarni ◽  
Sambhaji B. Chavan ◽  
Mandar S. Deshpande ◽  
Dhanishta Sagotra ◽  
Pramod S. Kumbhar ◽  
...  
Keyword(s):  
Mixed Culture ◽  
Rice Field ◽  
Phb Production

Production of poly-3-hydroxybutyrate from mixed culture

Biologia ◽  
2016 ◽  
Vol 71 (7) ◽  
Author(s):  
Thomas Shalin ◽  
Raveendran Sindhu ◽  
Ashok Pandey ◽  
Vincenza Faraco ◽  
Parameswaran Binod
Keyword(s):  
Mixed Culture ◽  
Biodegradable Polymers ◽  
Biodegradable Polymer ◽  
The Other ◽  
Cost Difference ◽  
Microbial Cultures ◽  
Phb Production ◽  
Mixed Microbial Cultures ◽  
Main Barrier ◽  
The Cost

AbstractPoly-3-hydroxybutyrate (PHB) is a biodegradable polymer produced by many bacteria. Some of the properties are similar to thermoplastics like polypropylene, hence finding application PHB can directly replace non-biodegradable polymers. But the main barrier has been the cost difference. The utilization of mixed microbial cultures facilitates the use of complex substrates and thus can reduce the cost of PHB production. In the present study, mixed culture systems, where metabolite produced by one organism may be assimilated by the other organism, were employed.

Growth of a Methane-Utilizing Mixed Culture HD6T on Methanol and Poly-β-Hydroxybutyrate Biosynthesis

2010 ◽  
Vol 160-162 ◽  
pp. 171-175 ◽  
Author(s):  
Jing Dong ◽  
Jia Ying Xin ◽  
Ying Xin Zhang ◽  
Lin Lin Chen ◽  
Hong Ye Liang ◽  
...  
Keyword(s):  
Mixed Culture ◽  
Mineral Salt Medium ◽  
Biomass Concentration ◽  
Dry Weight ◽  
Maximum Growth ◽  
Maximum Growth Rate ◽  
Production Costs ◽  
Lag Phase ◽  
Phase Duration ◽  
Phb Production

Methane-utilizing mixed culture HD6T was successfully cultivated in a brief non-sterile process using methanol as a sole carbon and energy source for the production of poly-β-hydroxybutyrate(PHB). Shake-flask experiments showed HD6T could grow well in the mineral salt medium with the addition of methanol exposed to the air directly. This non-sterile process and the use of cheap substrates (methanol) can reduce the production costs of PHB. It was found that HD6T grew better and PHB production in a more effective way with an initial liquid methanol concentration of 0.15%(v/v).The lag phase duration, the maximum growth rate, the biomass concentration and the PHB yield, for the optimal conditions were, respectively, 12.03h, 0.04h-1(OD600), 1.54g/l(dry weight), 0.424g/l(dry weight). Methane-utilizing mixed culture HD6T appears to be a promising organism for PHB production.

Establishment of Fermentation Cultivation System to Produce PHB Using Methane-Utilizing Mixed Culture

2012 ◽  
Vol 200 ◽  
pp. 389-392
Author(s):  
Jing Dong ◽  
He Teng Wang
Keyword(s):  
Mixed Culture ◽  
High Cell Concentration ◽  
Cultivation System ◽  
Fermentation Method ◽  
Phb Production ◽  
Fermentation Tank ◽  
Shake Flasks ◽  
One Step ◽  
Fermentation Strategy ◽  
Good Productivity

A new fermentation strategy, one-step fermentation was developed for PHB production with high cell concentration and good productivity in a simplified way. Combined with the research results of PHB synthesis with the method of shake-flasks fermentation cultivation, the fermentation system was built based on the characteristics of fermentation tank cultivation,which was much superior to the traditional two-step fermentation. Based on shake flask studies, the full advantages of fermentation culture was taken to achieve the improvement of PHB production. Through this fermentation method with high PHB accumulation capacity, it provides a feasible way to expand the scale of PHB production, and promotes PHB industrial production process using Methane-Utilizing mixed Culture.

scholarly journals Optimizing PHB and Protease Production by Box Behnken Design

2013 ◽  
Vol 14 (1) ◽  
Author(s):  
Amro Abd al fattah Amara
Keyword(s):  
Experimental Design ◽  
Mixed Culture ◽  
Protease Production ◽  
Optimum Conditions ◽  
Model Accuracy ◽  
Box Behnken Design ◽  
Phb Production ◽  
Complicated Process ◽  
Excel Solver ◽  
Production Conditions

Mixed culture is more suitable to adapt more flexible fermentation process and produce different product simultaneously. In this study a mixed Bacillus culture was investigated for their ability to produce the bioplastic "Polyhydroxybutyrate" and both of the mesophilic and the thermophilic proteases in one flask. Box-Behnken experimental design was used. The produced amount of PHB has been increased significantly. Meanwhile there is a competition between PHB and proteases. The maximum produced amount of PHB using Box-Behnken design was 2.82 g/l/48 h with protease activity equal to 41.9 Units/ml/48 h for thermophilic proteases and 99.65 Units/ml/48 h for mesophilic proteases. Excel solver was used for extra-optimization for the optimum conditions obtained from Box-Behnken experiments and its model. The maximum PHB obtained after using Excel solver was 2.88 g/l/48 h. The maximum mesophilic and thermophilic activities obtained at the same PHB production conditions were 175.68 and 243.38 Units/ml respectively. The model accuracy as obtained from Excel solver was 118.8%, which prove the power of the experimental design in optimizing such complicated process. The strategies used in this study are recommended for the production of PHB and different proteases simultaneously using Bacillus mixed culture. ABSTRAK: Kultur campuran adalah lebih sesuai bagi proses penapaian yang fleksibel dan ia boleh menghasilkan produk yang berbeza secara serentak. Dalam kajian ini keupayaan  menghasilkan "Polyhydroxybutyrate" bioplastik serta mesofilik dan termofilik protease dalam satu flask oleh  kultur Bacillus campuran telah disiasat. Eksperimen rekabentuk Box-Behnken telah digunakan. Jumlah PHB yang dikeluarkan meningkat dengan ketara dan terdapat persaingan antara PHB dan protease. Jumlah keluaran PHB maksima menggunakan rekabentuk Box-Behnken adalah 2.82 g/l/48 jam dengan aktiviti protease sama dengan 41.9 Unit/ml/48 jam untuk protease termofilik dan 99.65 Unit/ml/48 h untuk protease mesofilik. Solver Excel telah digunakan untuk memperolehi kadar optimum tambahan dari keadaan optimum yang diperolehi dari experimen dan model Box-Behnken. PHB maksimum diperolehi setelah menggunakan solver Excel adalah 2.88 g/l/48 jam. Aktiviti mesofilik dan termofilik maksima diperolehi daripada keadaan pengeluaran PHB yang sama adalah 175.68 dan 243.38 Unit / ml. Ketepatan model diperolehi daripada solver Excel adalah 118.8%, membuktikan kekuatan eksperimen tersebut bagi mengoptimumkan proses yang rumit. Strategi yang digunakan dalam kajian ini adalah disyorkan bagi pengeluaran PHB dan proteas berbeza secara serentak menggunakan kultur Bacillus campuran.KEYWORDS: PHB; proteases; mixed culture; Box-Behnken design.

Modeling of the mixed culture and periodic control for PHB production

2002 ◽  
Vol 10 (3) ◽  
pp. 157-173 ◽  
Author(s):  
Masayuki Tohyama ◽  
Trayanka Patarinska ◽  
Ziwen Qiang ◽  
Kazuyuki Shimizu
Keyword(s):  
Mixed Culture ◽  
Periodic Control ◽  
Phb Production
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