scholarly journals 431. Large-Scale Production of Lentiviral Vectors Using Adherent and Suspension-Adapted Cells

2007 ◽  
Vol 15 ◽  
pp. S167
Pharmaceutics ◽  
2020 ◽  
Vol 12 (11) ◽  
pp. 1051
Author(s):  
Eduardo Martínez-Molina ◽  
Carlos Chocarro-Wrona ◽  
Daniel Martínez-Moreno ◽  
Juan A. Marchal ◽  
Houria Boulaiz

Lentiviral vectors (LVs) have gained value over recent years as gene carriers in gene therapy. These viral vectors are safer than what was previously being used for gene transfer and are capable of infecting both dividing and nondividing cells with a long-term expression. This characteristic makes LVs ideal for clinical research, as has been demonstrated with the approval of lentivirus-based gene therapies from the Food and Drug Administration and the European Agency for Medicine. A large number of functional lentiviral particles are required for clinical trials, and large-scale production has been challenging. Therefore, efforts are focused on solving the drawbacks associated with the production and purification of LVsunder current good manufacturing practice. In recent years, we have witnessed the development and optimization of new protocols, packaging cell lines, and culture devices that are very close to reaching the target production level. Here, we review the most recent, efficient, and promising methods for the clinical-scale production ofLVs.


2008 ◽  
Vol 16 (3) ◽  
pp. 500-507 ◽  
Author(s):  
Sophie Broussau ◽  
Nadine Jabbour ◽  
Guillaume Lachapelle ◽  
Yves Durocher ◽  
Rosanne Tom ◽  
...  

2018 ◽  
Vol 5 (2) ◽  
pp. 90 ◽  
Author(s):  
Nathan Rout-Pitt ◽  
Alexandra McCarron ◽  
Chantelle McIntyre ◽  
David Parsons ◽  
Martin Donnelley

2016 ◽  
Vol 24 ◽  
pp. S279-S280
Author(s):  
Alexandre Audy ◽  
Marc-André Robert ◽  
Parminder Chahal ◽  
Rénald Gilbert ◽  
Bruno Gaillet

2003 ◽  
Vol 12 (3) ◽  
pp. 221-228 ◽  
Author(s):  
Jason E. Coleman ◽  
Matthew J. Huentelman ◽  
Sergey Kasparov ◽  
Beverly L. Metcalfe ◽  
Julian F. R. Paton ◽  
...  

The aim of this study was to develop an efficient method for packaging and concentrating lentiviral vectors that consistently yields high-titer virus on a scale suitable for in vivo applications. Transient cotransfection of 293T packaging cells with DNA plasmids encoding lentiviral vector components was optimized using SuperFect, an activated dendrimer-based transfection reagent. The use of SuperFect allowed reproducible and efficient production of high-titer lentiviral vector at concentrations greater than 1 × 107transducing units per ml (TU/ml) and required less than one-third of the total amount of DNA used in traditional calcium phosphate transfection methods. Viral titers were further increased using a novel concentration protocol that yielded an average final titer of 1.4 × 1010TU/ml. Lentiviruses produced using these methods exhibited efficient transduction of central nervous system and peripheral tissues in vivo. The method is reproducible and can be scaled up to facilitate the use of these vectors in animal studies.


2003 ◽  
Vol 14 (1) ◽  
pp. 67-77 ◽  
Author(s):  
Mukesh Kumar ◽  
Brian P. Bradow ◽  
Joshua Zimmerberg

1993 ◽  
Vol 32 (1) ◽  
pp. 129-131
Author(s):  
Naureen Talha

The literature on female labour in Third World countries has become quite extensive. India, being comparatively more advanced industrially, and in view of its size and population, presents a pictures of multiplicity of problems which face the female labour market. However, the author has also included Mexico in this analytical study. It is interesting to see the characteristics of developing industrialisation in two different societies: the Indian society, which is conservative, and the Mexican society, which is progressive. In the first chapter of the book, the author explains that he is not concerned with the process of industrialisation and female labour employed at different levels of work, but that he is interested in forms of production and women's employment in large-scale production, petty commodity production, marginal small production, and self-employment in the informal sector. It is only by analysis of these forms that the picture of females having a lower status is understood in its social and political setting.


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